Gene description for ADAMTS13
Gene name ADAM metallopeptidase with thrombospondin type 1 motif, 13
Gene symbol ADAMTS13
Other names/aliases ADAM-TS13
ADAMTS-13
C9orf8
VWFCP
vWF-CP
Species Homo sapiens
 Database cross references - ADAMTS13
ExoCarta ExoCarta_11093
Vesiclepedia VP_11093
Entrez Gene 11093
HGNC 1366
MIM 604134
UniProt Q76LX8  
 ADAMTS13 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
 Gene ontology annotations for ADAMTS13
Molecular Function
    metalloendopeptidase activity GO:0004222 EXP
    metalloendopeptidase activity GO:0004222 IBA
    metalloendopeptidase activity GO:0004222 TAS
    integrin binding GO:0005178 TAS
    calcium ion binding GO:0005509 TAS
    protein binding GO:0005515 IPI
    metallopeptidase activity GO:0008237 TAS
    zinc ion binding GO:0008270 TAS
Biological Process
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 IDA
    cell-matrix adhesion GO:0007160 NAS
    integrin-mediated signaling pathway GO:0007229 NAS
    blood coagulation GO:0007596 TAS
    glycoprotein metabolic process GO:0009100 NAS
    response to toxic substance GO:0009636 IEA
    response to amine GO:0014075 IEA
    protein processing GO:0016485 TAS
    protein catabolic process GO:0030163 IEA
    platelet activation GO:0030168 NAS
    extracellular matrix organization GO:0030198 IBA
    response to potassium ion GO:0035864 IEA
    peptide catabolic process GO:0043171 IDA
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to type II interferon GO:0071346 IEA
    cellular response to interleukin-4 GO:0071353 IEA
    cellular response to tumor necrosis factor GO:0071356 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IEA
    endoplasmic reticulum lumen GO:0005788 TAS
    cell surface GO:0009986 NAS
    extracellular matrix GO:0031012 IBA
    extracellular matrix GO:0031012 TAS
 Experiment description of studies that identified ADAMTS13 in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
6
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for ADAMTS13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HERC1 8925
Affinity Capture-MS Homo sapiens
2 KIAA0232 9778
Affinity Capture-MS Homo sapiens
3 FZD3  
Affinity Capture-MS Homo sapiens
4 C2CD4B  
Affinity Capture-MS Homo sapiens
5 KLHL8  
Affinity Capture-MS Homo sapiens
6 B3GALTL 145173
Affinity Capture-MS Homo sapiens
7 SMTNL1  
Affinity Capture-MS Homo sapiens
8 CELSR1 9620
Affinity Capture-MS Homo sapiens
9 FREM2 341640
Affinity Capture-MS Homo sapiens
10 TARBP2  
Affinity Capture-MS Homo sapiens
11 FAT4 79633
Affinity Capture-MS Homo sapiens
12 PCDH7 5099
Affinity Capture-MS Homo sapiens
13 FAT3 120114
Affinity Capture-MS Homo sapiens
14 ALDH18A1 5832
Affinity Capture-MS Homo sapiens
15 ZNF496  
Affinity Capture-MS Homo sapiens
16 KIAA0754  
Affinity Capture-MS Homo sapiens
17 CELSR2  
Affinity Capture-MS Homo sapiens
18 MPP5 64398
Affinity Capture-MS Homo sapiens
19 AGGF1  
Affinity Capture-MS Homo sapiens
20 DICER1  
Affinity Capture-MS Homo sapiens
21 ARMCX3 51566
Affinity Capture-MS Homo sapiens
22 PDGFC 56034
Affinity Capture-MS Homo sapiens
23 PASK  
Affinity Capture-MS Homo sapiens
24 TCF25 22980
Affinity Capture-MS Homo sapiens
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