Gene description for Eif4a3
Gene name eukaryotic translation initiation factor 4A3
Gene symbol Eif4a3
Other names/aliases 2400003O03Rik
Ddx48
eIF4A-III
mKIAA0111
Species Mus musculus
 Database cross references - Eif4a3
ExoCarta ExoCarta_192170
Vesiclepedia VP_192170
Entrez Gene 192170
UniProt Q91VC3  
 Eif4a3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif4a3
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 IEA
    RNA helicase activity GO:0003724 IBA
    RNA helicase activity GO:0003724 IEA
    RNA helicase activity GO:0003724 ISO
    mRNA binding GO:0003729 IBA
    mRNA binding GO:0003729 ISO
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    poly(A) binding GO:0008143 ISO
    ATP hydrolysis activity GO:0016887 IEA
    selenocysteine insertion sequence binding GO:0035368 ISO
    RNA stem-loop binding GO:0035613 ISO
    ribonucleoprotein complex binding GO:0043021 ISO
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IEA
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 ISO
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 ISS
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    chromatin remodeling GO:0006338 IEA
    rRNA processing GO:0006364 IEA
    mRNA export from nucleus GO:0006406 NAS
    regulation of translation GO:0006417 IEA
    negative regulation of gene expression GO:0010629 ISO
    negative regulation of translation GO:0017148 ISO
    positive regulation of translation GO:0045727 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of mRNA splicing, via spliceosome GO:0048026 IMP
    embryonic cranial skeleton morphogenesis GO:0048701 ISO
    embryonic cranial skeleton morphogenesis GO:0048701 ISS
    negative regulation of excitatory postsynaptic potential GO:0090394 ISO
    regulation of translation at postsynapse, modulating synaptic transmission GO:0099578 ISO
    chromatin looping GO:0140588 IEA
    negative regulation of selenocysteine incorporation GO:1904570 ISO
    regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:2000622 ISO
    regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:2000622 ISS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    nuclear speck GO:0016607 IEA
    dendrite GO:0030425 ISO
    exon-exon junction complex GO:0035145 ISO
    exon-exon junction complex GO:0035145 ISS
    neuronal cell body GO:0043025 ISO
    U2-type catalytic step 1 spliceosome GO:0071006 ISO
    U2-type catalytic step 1 spliceosome GO:0071006 ISS
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 ISO
    glutamatergic synapse GO:0098978 ISO
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Eif4a3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif4a3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 Ddx5 13207
Co-fractionation Mus musculus
3 Erh  
Co-fractionation Mus musculus
4 Prpf8 192159
Co-fractionation Mus musculus
5 Wdr77  
Co-fractionation Mus musculus
6 Rbm8a  
Co-fractionation Mus musculus
7 Cebpa  
Affinity Capture-MS Mus musculus
8 Tfe3  
Affinity Capture-MS Mus musculus
9 Snrnp200 320632
Co-fractionation Mus musculus
10 Frg1  
Co-fractionation Mus musculus
11 Ddb1 13194
Affinity Capture-MS Mus musculus
12 EIF4A3 9775
Affinity Capture-MS Homo sapiens
13 Sf3b3 101943
Co-fractionation Mus musculus
14 Hnrnpc 15381
Co-fractionation Mus musculus
15 Srrm2  
Co-fractionation Mus musculus
16 Hnrnpa3 229279
Co-fractionation Mus musculus
17 Plrg1  
Co-fractionation Mus musculus
18 Snw1  
Co-fractionation Mus musculus
19 Ppwd1  
Co-fractionation Mus musculus
20 Fancd2  
Affinity Capture-MS Mus musculus
21 Ppil3  
Co-fractionation Mus musculus
22 Eed  
Affinity Capture-MS Mus musculus
23 Cwc27  
Co-fractionation Mus musculus
24 Skiv2l2  
Co-fractionation Mus musculus
25 Eftud2 20624
Co-fractionation Mus musculus
26 Hnrnpa2b1 53379
Co-fractionation Mus musculus
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