ExoCarta: Gene summary

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Gene description for Rpl9
Gene name	ribosomal protein L9
Gene symbol	Rpl9
Other names/aliases	-
Species	Mus musculus
Database cross references - Rpl9
ExoCarta	ExoCarta_20005
Vesiclepedia	VP_20005
Entrez Gene	20005
UniProt	P51410
Rpl9 identified in sEVs derived from the following tissue/cell type
Colon cancer cells	37309723
Fibroblasts	23260141
Mast cells	17486113
T-cell lymphoma cells	37309723

Gene ontology annotations for Rpl9

Molecular Function
	structural constituent of ribosome	GO:0003735	IBA
	structural constituent of ribosome	GO:0003735	IC
	structural constituent of ribosome	GO:0003735	IDA
	structural constituent of ribosome	GO:0003735	ISO
	rRNA binding	GO:0019843	IEA
Biological Process
	cytoplasmic translation	GO:0002181	IBA
	cytoplasmic translation	GO:0002181	NAS
	translation	GO:0006412	IC
	translation at presynapse	GO:0140236	NAS
	translation at postsynapse	GO:0140242	NAS
Subcellular Localization
	cytoplasm	GO:0005737	IDA
	cytosol	GO:0005829	TAS
	ribosome	GO:0005840	NAS
	cytosolic large ribosomal subunit	GO:0022625	IBA
	cytosolic large ribosomal subunit	GO:0022625	IDA
	cytosolic large ribosomal subunit	GO:0022625	IPI
	cytosolic large ribosomal subunit	GO:0022625	ISO
	cytosolic ribosome	GO:0022626	ISO
	synapse	GO:0045202	EXP
	synapse	GO:0045202	IDA
	presynapse	GO:0098793	NAS
	postsynapse	GO:0098794	NAS

Experiment description of studies that identified Rpl9 in sEVs

Experiment ID

907

MISEV standards
✔ EM	Biophysical techniques
✔ Tsg101\|Sdcbp\|Cd151\|Gapdh\|Lamp2\|Cd81\|Cd82\|Cd9\|Cd63\|Cd80\|Flot2\|Tfrc\|Rab35\|Rab5a	Enriched markers
✔ HSPA5	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

37309723

Organism

Mus musculus

Experiment description

Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells

Authors

"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."

Journal name

Proteomics

Publication year

2023

Sample

Colon cancer cells

Sample name

C26

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

210

MISEV standards
✔ EM	Biophysical techniques
✔ CD81\|FLOT1	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23260141

Organism

Mus musculus

Experiment description

Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.

Authors

"Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."

Journal name

Cell

Publication year

2012

Sample

Fibroblasts

Sample name

Normal-Fibroblasts (L cells)

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

MISEV standards
✔ EM	Biophysical techniques
✔ CD63	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

mrna

Identification method

Microarray

PubMed ID

17486113

Organism

Mus musculus
Homo sapiens

Experiment description

Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.

Authors

"Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"

Journal name

NCB

Publication year

2007

Sample

Mast cells

Sample name

MC9
Bone marrow-derived mast cells
HMC-1

Isolation/purification methods

Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.11-1.21 g/mL

Molecules identified in the study

Protein
mRNA
miRNA

Methods used in the study

Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array

Experiment ID

908

MISEV standards
✔ EM	Biophysical techniques
✔ Tsg101\|Sdcbp\|Cd151\|Gapdh\|Lamp2\|Cd81\|Cd82\|Cd9\|Cd63\|Cd80\|Flot2\|Tfrc\|Rab35\|Rab5a	Enriched markers
✔ HSPA5	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

37309723

Organism

Mus musculus

Experiment description

Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells

Authors

"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."

Journal name

Proteomics

Publication year

2023

Sample

T-cell lymphoma cells

Sample name

EL4

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Protein-protein interactions for Rpl9

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

Rps3

27050

Co-fractionation

Mus musculus

Rpl11

67025

Co-fractionation

Mus musculus

Elf5

Affinity Capture-MS

Mus musculus

Rpl31

114641

Co-fractionation

Mus musculus

Eif5a

276770

Co-fractionation

Mus musculus

Rplp2

67186

Co-fractionation

Mus musculus

Atxn1

Affinity Capture-MS

Mus musculus

Tfe3

Affinity Capture-MS

Mus musculus

Rps7

20115

Co-fractionation

Mus musculus

Tmem173

Proximity Label-MS

Mus musculus

Fzr1

Affinity Capture-MS

Mus musculus

Tardbp

230908

Co-fractionation

Mus musculus

Eef2

13629

Co-fractionation

Mus musculus

Rpl12

269261

Co-fractionation

Mus musculus

Rpl18

19899

Co-fractionation

Mus musculus

Cdkn2a

Two-hybrid

Mus musculus

Rps15a

267019

Co-fractionation

Mus musculus

Rps5

20103

Co-fractionation

Mus musculus

Rps21

66481

Co-fractionation

Mus musculus

Rps14

20044

Co-fractionation

Mus musculus

Rpl30

19946

Co-fractionation

Mus musculus

Eed

Affinity Capture-MS

Mus musculus

Rpsa

16785

Co-fractionation

Mus musculus

Igsf8

140559

Co-fractionation

Mus musculus

Rpl5

100503670

Co-fractionation

Mus musculus

Fancd2

Affinity Capture-MS

Mus musculus

View the network

Pathways in which Rpl9 is involved

Pathway	Evidence	Source
Cap-dependent Translation Initiation	IEA	Reactome
Eukaryotic Translation Initiation	IEA	Reactome
Formation of a pool of free 40S subunits	IEA	Reactome
GTP hydrolysis and joining of the 60S ribosomal subunit	IEA	Reactome
L13a-mediated translational silencing of Ceruloplasmin expression	IEA	Reactome
Major pathway of rRNA processing in the nucleolus and cytosol	IEA	Reactome
Metabolism of proteins	IEA	Reactome
Metabolism of RNA	IEA	Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)	IEA	Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)	IEA	Reactome
Nonsense-Mediated Decay (NMD)	IEA	Reactome
rRNA processing	IEA	Reactome
rRNA processing in the nucleus and cytosol	IEA	Reactome
SRP-dependent cotranslational protein targeting to membrane	IEA	Reactome
Translation	IEA	Reactome

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