Gene description for Rps7
Gene name ribosomal protein S7
Gene symbol Rps7
Other names/aliases Mtu
Rps7A
S7
Species Mus musculus
 Database cross references - Rps7
ExoCarta ExoCarta_20115
Vesiclepedia VP_20115
Entrez Gene 20115
UniProt P62082  
 Rps7 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rps7
Molecular Function
    mRNA 3'-UTR binding GO:0003730 IEA
    mRNA 3'-UTR binding GO:0003730 ISO
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    poly(U) RNA binding GO:0008266 ISO
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    mRNA 5'-UTR binding GO:0048027 IEA
    mRNA 5'-UTR binding GO:0048027 ISO
    ubiquitin ligase inhibitor activity GO:1990948 IEA
    ubiquitin ligase inhibitor activity GO:1990948 ISO
Biological Process
    neural tube closure GO:0001843 IMP
    cytoplasmic translation GO:0002181 NAS
    rRNA processing GO:0006364 IBA
    rRNA processing GO:0006364 IEA
    rRNA processing GO:0006364 ISO
    positive regulation of gene expression GO:0010628 IEA
    positive regulation of gene expression GO:0010628 ISO
    neural crest cell differentiation GO:0014033 IMP
    ribosomal small subunit biogenesis GO:0042274 IBA
    ribosomal small subunit biogenesis GO:0042274 ISO
    ribosomal small subunit biogenesis GO:0042274 ISS
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator GO:1902255 IEA
    positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator GO:1902255 ISO
    negative regulation of ubiquitin protein ligase activity GO:1904667 ISO
    negative regulation of ubiquitin-dependent protein catabolic process GO:2000059 IEA
    negative regulation of ubiquitin-dependent protein catabolic process GO:2000059 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 NAS
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    cytosolic ribosome GO:0022626 ISO
    cytosolic small ribosomal subunit GO:0022627 IBA
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytosolic small ribosomal subunit GO:0022627 ISO
    cytosolic small ribosomal subunit GO:0022627 NAS
    small-subunit processome GO:0032040 IBA
    small-subunit processome GO:0032040 ISO
    small-subunit processome GO:0032040 ISS
    protein-containing complex GO:0032991 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    postsynapse GO:0098794 NAS
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Rps7 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rps7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Rps3 27050
Co-fractionation Mus musculus
3 Rps26 27370
Co-fractionation Mus musculus
4 Rpl35 66489
Co-fractionation Mus musculus
5 Rps18 20084
Co-fractionation Mus musculus
6 SNCA 6622
Affinity Capture-MS Homo sapiens
7 Rps25 75617
Co-fractionation Mus musculus
8 Rps28 54127
Co-fractionation Mus musculus
9 Rps10 67097
Co-fractionation Mus musculus
10 Rpl13a 22121
Co-fractionation Mus musculus
11 Srrm1 51796
Co-fractionation Mus musculus
12 Eif2a  
Co-fractionation Mus musculus
13 Rplp2 67186
Co-fractionation Mus musculus
14 Rps4x 20102
Co-fractionation Mus musculus
15 Rpl14 67115
Co-fractionation Mus musculus
16 Rps2 16898
Co-fractionation Mus musculus
17 Rps8 20116
Co-fractionation Mus musculus
18 Rps23 66475
Co-fractionation Mus musculus
19 Rpl7a 27176
Co-fractionation Mus musculus
20 Rpl31 114641
Co-fractionation Mus musculus
21 Rpl11 67025
Co-fractionation Mus musculus
22 Rpl32 19951
Co-fractionation Mus musculus
23 Wibg  
Co-fractionation Mus musculus
24 Rpl6 19988
Co-fractionation Mus musculus
25 Kctd13  
Affinity Capture-MS Mus musculus
26 Rps19 20085
Co-fractionation Mus musculus
27 Tfe3  
Affinity Capture-MS Mus musculus
28 Rpl9 20005
Co-fractionation Mus musculus
29 Rps24 20088
Co-fractionation Mus musculus
30 Tpt1 22070
Co-fractionation Mus musculus
31 Rpgrip1l  
Affinity Capture-MS Mus musculus
32 Rps11 27207
Co-fractionation Mus musculus
33 Rpl18a 76808
Co-fractionation Mus musculus
34 Ube2o 217342
Co-fractionation Mus musculus
35 Rps5 20103
Co-fractionation Mus musculus
36 Rpl17 319195
Co-fractionation Mus musculus
37 Rpl22 19934
Co-fractionation Mus musculus
38 Rps16 20055
Co-fractionation Mus musculus
39 Fzr1  
Affinity Capture-MS Mus musculus
40 Rpl15 66480
Co-fractionation Mus musculus
41 Rps6 20104
Co-fractionation Mus musculus
42 Rps13 68052
Co-fractionation Mus musculus
43 Rpl12 269261
Co-fractionation Mus musculus
44 Rpl18 19899
Co-fractionation Mus musculus
45 Cdkn2a  
Two-hybrid Mus musculus
46 Rps21 66481
Co-fractionation Mus musculus
47 Rpl7 19989
Co-fractionation Mus musculus
48 Psmc4 23996
Co-fractionation Mus musculus
49 Rps15a 267019
Co-fractionation Mus musculus
50 Gnb2l1 14694
Co-fractionation Mus musculus
51 Rps15 20054
Co-fractionation Mus musculus
52 Eef1a1 13627
Co-fractionation Mus musculus
53 Rps3a1 20091
Co-fractionation Mus musculus
54 Npm1 18148
Affinity Capture-MS Mus musculus
55 Rpl26 19941
Co-fractionation Mus musculus
56 Hba 15121
Co-fractionation Mus musculus
57 Rplp0 11837
Co-fractionation Mus musculus
58 Rpl23a 268449
Co-fractionation Mus musculus
59 Rps14 20044
Co-fractionation Mus musculus
60 Rps17 20068
Co-fractionation Mus musculus
61 Rps9 76846
Co-fractionation Mus musculus
62 Eed  
Affinity Capture-MS Mus musculus
63 Rps27 57294
Co-fractionation Mus musculus
64 Rpsa 16785
Co-fractionation Mus musculus
65 Mcts1  
Co-fractionation Mus musculus
66 Eef1b2 55949
Co-fractionation Mus musculus
67 Rpl5 100503670
Co-fractionation Mus musculus
68 Rps20 67427
Co-fractionation Mus musculus
69 Rpl8 26961
Co-fractionation Mus musculus
70 Rps12 20042
Co-fractionation Mus musculus
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