Gene description for Rps3
Gene name ribosomal protein S3
Gene symbol Rps3
Other names/aliases D7Ertd795e
Rs_3
Species Mus musculus
 Database cross references - Rps3
ExoCarta ExoCarta_27050
Vesiclepedia VP_27050
Entrez Gene 27050
UniProt P62908  
 Rps3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
Mov neuroglial cells 15210972    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rps3
Molecular Function
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 ISO
    DNA binding GO:0003677 IEA
    DNA binding GO:0003677 ISO
    damaged DNA binding GO:0003684 ISO
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    mRNA binding GO:0003729 ISO
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    DNA-(apurinic or apyrimidinic site) endonuclease activity GO:0003906 IDA
    DNA-(apurinic or apyrimidinic site) endonuclease activity GO:0003906 ISO
    DNA endonuclease activity GO:0004520 ISO
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 ISO
    tubulin binding GO:0015631 ISO
    enzyme binding GO:0019899 ISO
    kinase binding GO:0019900 ISO
    protein kinase binding GO:0019901 ISO
    Hsp70 protein binding GO:0030544 ISO
    oxidized purine DNA binding GO:0032357 ISO
    oxidized pyrimidine DNA binding GO:0032358 ISO
    ubiquitin-like protein conjugating enzyme binding GO:0044390 ISO
    protein-containing complex binding GO:0044877 ISO
    protein kinase A binding GO:0051018 ISO
    Hsp90 protein binding GO:0051879 ISO
    small ribosomal subunit rRNA binding GO:0070181 ISO
    supercoiled DNA binding GO:0097100 ISO
    class I DNA-(apurinic or apyrimidinic site) endonuclease activity GO:0140078 IEA
    DNA-binding transcription factor binding GO:0140297 ISO
Biological Process
    cytoplasmic translation GO:0002181 NAS
    DNA repair GO:0006281 IEA
    DNA repair GO:0006281 ISO
    regulation of translation GO:0006417 IEA
    apoptotic process GO:0006915 IEA
    DNA damage response GO:0006974 ISO
    chromosome segregation GO:0007059 ISO
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of translation GO:0017148 ISO
    positive regulation of microtubule polymerization GO:0031116 ISO
    positive regulation of protein-containing complex assembly GO:0031334 IMP
    positive regulation of protein-containing complex assembly GO:0031334 ISO
    negative regulation of protein ubiquitination GO:0031397 ISO
    positive regulation of endodeoxyribonuclease activity GO:0032079 ISO
    positive regulation of interleukin-2 production GO:0032743 ISO
    cellular response to reactive oxygen species GO:0034614 ISO
    positive regulation of activated T cell proliferation GO:0042104 ISO
    regulation of apoptotic process GO:0042981 ISO
    positive regulation of JUN kinase activity GO:0043507 ISO
    negative regulation of DNA repair GO:0045738 ISO
    positive regulation of DNA repair GO:0045739 ISO
    positive regulation of T cell receptor signaling pathway GO:0050862 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    spindle assembly GO:0051225 ISO
    cell division GO:0051301 IEA
    response to TNF agonist GO:0061481 ISO
    cellular response to hydrogen peroxide GO:0070301 ISO
    cellular response to tumor necrosis factor GO:0071356 ISO
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 ISO
    positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage GO:1902231 ISO
    positive regulation of base-excision repair GO:1905053 ISO
    cellular response to nerve growth factor stimulus GO:1990090 ISO
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
    positive regulation of apoptotic signaling pathway GO:2001235 IBA
    positive regulation of apoptotic signaling pathway GO:2001235 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 NAS
    mitochondrial inner membrane GO:0005743 IEA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial matrix GO:0005759 ISO
    endoplasmic reticulum GO:0005783 ISO
    spindle GO:0005819 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    ribosome GO:0005840 ISO
    ribosome GO:0005840 NAS
    plasma membrane GO:0005886 ISO
    postsynaptic density GO:0014069 ISO
    cytosolic ribosome GO:0022626 ISO
    cytosolic small ribosomal subunit GO:0022627 IBA
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytosolic small ribosomal subunit GO:0022627 ISO
    cytosolic small ribosomal subunit GO:0022627 NAS
    dendrite GO:0030425 ISO
    ruffle membrane GO:0032587 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    NF-kappaB complex GO:0071159 ISO
    mitotic spindle GO:0072686 ISO
    postsynapse GO:0098794 NAS
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Rps3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rps3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
2 Atxn2 20239
Co-fractionation Mus musculus
3 Dhx29  
Co-fractionation Mus musculus
4 Raver1  
Co-fractionation Mus musculus
5 Ybx1 22608
Co-fractionation Mus musculus
6 Fmr1  
Co-fractionation Mus musculus
7 Rpl6 19988
Co-fractionation Mus musculus
8 Cebpa  
Affinity Capture-MS Mus musculus
9 Rpl9 20005
Co-fractionation Mus musculus
10 Rps24 20088
Co-fractionation Mus musculus
11 Ncl 17975
Co-fractionation Mus musculus
12 Actb 11461
Co-fractionation Mus musculus
13 Tmem173  
Proximity Label-MS Mus musculus
14 Larp1  
Co-fractionation Mus musculus
15 Rpl7 19989
Co-fractionation Mus musculus
16 Eef1a1 13627
Co-fractionation Mus musculus
17 Unk  
Affinity Capture-RNA Mus musculus
18 Sra1  
Co-fractionation Mus musculus
19 Rpl26 19941
Co-fractionation Mus musculus
20 Rpsa 16785
Co-fractionation Mus musculus
21 Rpl3 27367
Co-fractionation Mus musculus
22 Rpl11 67025
Co-fractionation Mus musculus
23 Eed  
Affinity Capture-MS Mus musculus
24 Rpl31 114641
Co-fractionation Mus musculus
25 Rps10 67097
Co-fractionation Mus musculus
26 Stau1  
Co-fractionation Mus musculus
27 Rplp2 67186
Co-fractionation Mus musculus
28 Rps4x 20102
Co-fractionation Mus musculus
29 Wibg  
Co-fractionation Mus musculus
30 Trove2 20822
Co-fractionation Mus musculus
31 Rps23 66475
Co-fractionation Mus musculus
32 Mapt  
Affinity Capture-MS Mus musculus
33 Tfe3  
Affinity Capture-MS Mus musculus
34 Rps7 20115
Co-fractionation Mus musculus
35 Rpl17 319195
Co-fractionation Mus musculus
36 Pabpc1 18458
Co-fractionation Mus musculus
37 Rps5 20103
Co-fractionation Mus musculus
38 Actn4 60595
Co-fractionation Mus musculus
39 Rps15a 267019
Co-fractionation Mus musculus
40 Nanog  
Affinity Capture-MS Mus musculus
41 Fancd2  
Affinity Capture-MS Mus musculus
42 Rplp0 11837
Co-fractionation Mus musculus
43 Rps16 20055
Co-fractionation Mus musculus
44 Rps17 20068
Co-fractionation Mus musculus
45 Tubgcp4 51885
Co-fractionation Mus musculus
46 Tcf3  
Affinity Capture-MS Mus musculus
47 Nphs2  
Affinity Capture-MS Mus musculus
48 Ubc  
Reconstituted Complex Mus musculus
49 Rps18 20084
Co-fractionation Mus musculus
50 Rpl13a 22121
Co-fractionation Mus musculus
51 Rpl14 67115
Co-fractionation Mus musculus
52 Rps2 16898
Co-fractionation Mus musculus
53 Gapdh 14433
Co-fractionation Mus musculus
54 Rps8 20116
Co-fractionation Mus musculus
55 Hectd1  
Affinity Capture-Western Mus musculus
56 Rpl7a 27176
Co-fractionation Mus musculus
57 Rpl32 19951
Co-fractionation Mus musculus
58 Rps28 54127
Co-fractionation Mus musculus
59 Polrmt  
Two-hybrid Mus musculus
60 Rps19 20085
Co-fractionation Mus musculus
61 Calr 12317
Co-fractionation Mus musculus
62 Tpt1 22070
Co-fractionation Mus musculus
63 Rps11 27207
Co-fractionation Mus musculus
64 Rpl18a 76808
Co-fractionation Mus musculus
65 Ago1 236511
Co-fractionation Mus musculus
66 Ywhae 22627
Affinity Capture-MS Mus musculus
67 Rpl22 19934
Co-fractionation Mus musculus
68 Rpl19 19921
Co-fractionation Mus musculus
69 Rps6 20104
Co-fractionation Mus musculus
70 Hnrnpab 15384
Co-fractionation Mus musculus
71 Dmrt2  
Affinity Capture-MS Mus musculus
72 Dhx9 13211
Co-fractionation Mus musculus
73 Syncrip 56403
Co-fractionation Mus musculus
74 Rps3a1 20091
Co-fractionation Mus musculus
75 Rps21 66481
Co-fractionation Mus musculus
76 Rps14 20044
Co-fractionation Mus musculus
77 Rpl35 66489
Co-fractionation Mus musculus
78 Tia1  
Affinity Capture-MS Mus musculus
79 Rpl5 100503670
Co-fractionation Mus musculus
80 Rps20 67427
Co-fractionation Mus musculus
81 Rps12 20042
Co-fractionation Mus musculus
82 Rps27a 78294
Co-fractionation Mus musculus
83 Rps25 75617
Co-fractionation Mus musculus
84 Kctd13  
Affinity Capture-MS Mus musculus
85 Rpgrip1l  
Affinity Capture-MS Mus musculus
86 Serbp1 66870
Co-fractionation Mus musculus
87 Fzr1  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
88 Rpl12 269261
Co-fractionation Mus musculus
89 Rpl18 19899
Co-fractionation Mus musculus
90 Gnb2l1 14694
Co-fractionation Mus musculus
91 Rps15 20054
Co-fractionation Mus musculus
92 Npm1 18148
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
93 Rps13 68052
Co-fractionation Mus musculus
94 Eef1b2 55949
Co-fractionation Mus musculus
95 Rpl4 67891
Co-fractionation Mus musculus
96 Hnrnpa2b1 53379
Co-fractionation Mus musculus
97 Rpl8 26961
Co-fractionation Mus musculus
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