Gene description for Rpl17
Gene name ribosomal protein L17
Gene symbol Rpl17
Other names/aliases -
Species Mus musculus
 Database cross references - Rpl17
ExoCarta ExoCarta_319195
Vesiclepedia VP_319195
Entrez Gene 319195
 Rpl17 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl17
Molecular Function
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    protein binding GO:0005515 IPI
    large ribosomal subunit rRNA binding GO:0070180 ISO
Biological Process
    cytoplasmic translation GO:0002181 NAS
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 ISO
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    A band GO:0031672 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
 Experiment description of studies that identified Rpl17 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl17
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Rpl28 19943
Co-fractionation Mus musculus
3 Rpl35 66489
Co-fractionation Mus musculus
4 Cbx1  
Affinity Capture-MS Mus musculus
5 Rpl31 114641
Co-fractionation Mus musculus
6 Rps25 75617
Co-fractionation Mus musculus
7 Rps28 54127
Co-fractionation Mus musculus
8 Rpl11 67025
Co-fractionation Mus musculus
9 Rpl13a 22121
Co-fractionation Mus musculus
10 Rplp2 67186
Co-fractionation Mus musculus
11 Rpl38  
Co-fractionation Mus musculus
12 Rpl4 67891
Co-fractionation Mus musculus
13 Rpl14 67115
Co-fractionation Mus musculus
14 Rps2 16898
Co-fractionation Mus musculus
15 Rpl10a 19896
Co-fractionation Mus musculus
16 Rpl7a 27176
Co-fractionation Mus musculus
17 Rps18 20084
Co-fractionation Mus musculus
18 Rpl27 19942
Co-fractionation Mus musculus
19 Rpl6 19988
Co-fractionation Mus musculus
20 Kctd13  
Affinity Capture-MS Mus musculus
21 Tfe3  
Affinity Capture-MS Mus musculus
22 Rpl24 68193
Co-fractionation Mus musculus
23 Eif4a1 13681
Co-fractionation Mus musculus
24 Rpl18a 76808
Co-fractionation Mus musculus
25 Tpt1 22070
Co-fractionation Mus musculus
26 Rps7 20115
Co-fractionation Mus musculus
27 Rpl22 19934
Co-fractionation Mus musculus
28 Rps16 20055
Co-fractionation Mus musculus
29 Fzr1  
Affinity Capture-MS Mus musculus
30 Rpl12 269261
Co-fractionation Mus musculus
31 Rpl18 19899
Co-fractionation Mus musculus
32 Rpl21 19933
Co-fractionation Mus musculus
33 Rpl7 19989
Co-fractionation Mus musculus
34 Rplp0 11837
Co-fractionation Mus musculus
35 Cbx3 12417
Affinity Capture-MS Mus musculus
36 Rpl36a 19982
Co-fractionation Mus musculus
37 Rpl26 19941
Co-fractionation Mus musculus
38 Fancd2  
Affinity Capture-MS Mus musculus
39 Rpl23 65019
Co-fractionation Mus musculus
40 Rpl23a 268449
Co-fractionation Mus musculus
41 Lmo2  
Two-hybrid Mus musculus
42 G3bp1  
Co-fractionation Mus musculus
43 Eed  
Affinity Capture-MS Mus musculus
44 Rpl15 66480
Co-fractionation Mus musculus
45 Rpl8 26961
Co-fractionation Mus musculus
46 Rpl3 27367
Co-fractionation Mus musculus
47 Rpl5 100503670
Co-fractionation Mus musculus
48 Rpl13 270106
Co-fractionation Mus musculus
49 Rpl30 19946
Co-fractionation Mus musculus
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