Gene description for Rpl5
Gene name ribosomal protein L5
Gene symbol Rpl5
Other names/aliases U21RNA
Species Mus musculus
 Database cross references - Rpl5
ExoCarta ExoCarta_100503670
Vesiclepedia VP_100503670
Entrez Gene 100503670
UniProt P47962  
 Rpl5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl5
Molecular Function
    mRNA binding GO:0003729 ISO
    mRNA 3'-UTR binding GO:0003730 ISO
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    protein binding GO:0005515 IPI
    5S rRNA binding GO:0008097 IBA
    5S rRNA binding GO:0008097 IEA
    5S rRNA binding GO:0008097 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    mRNA 5'-UTR binding GO:0048027 ISO
    ubiquitin ligase inhibitor activity GO:1990948 ISO
Biological Process
    ribosomal large subunit assembly GO:0000027 IBA
    ribosomal large subunit assembly GO:0000027 ISO
    cytoplasmic translation GO:0002181 NAS
    rRNA processing GO:0006364 ISO
    positive regulation of gene expression GO:0010628 ISO
    ribosomal large subunit biogenesis GO:0042273 ISO
    positive regulation of translation GO:0045727 ISO
    protein stabilization GO:0050821 ISO
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
    regulation of signal transduction by p53 class mediator GO:1901796 ISO
    negative regulation of ubiquitin protein ligase activity GO:1904667 ISO
    negative regulation of ubiquitin-dependent protein catabolic process GO:2000059 ISO
    negative regulation of protein neddylation GO:2000435 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 ISO
    nucleolus GO:0005730 ISS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    endoplasmic reticulum GO:0005783 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    postsynaptic density GO:0014069 ISO
    aminoacyl-tRNA synthetase multienzyme complex GO:0017101 ISO
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    protein-containing complex GO:0032991 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Rpl5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Rps3 27050
Co-fractionation Mus musculus
3 Rpl11 67025
Co-fractionation Mus musculus
4 Rpl35 66489
Co-fractionation Mus musculus
5 Elf5  
Affinity Capture-MS Mus musculus
6 Rpl31 114641
Co-fractionation Mus musculus
7 Rps25 75617
Co-fractionation Mus musculus
8 Rps15a 267019
Co-fractionation Mus musculus
9 Rpl28 19943
Co-fractionation Mus musculus
10 Rpl13a 22121
Co-fractionation Mus musculus
11 Rplp2 67186
Co-fractionation Mus musculus
12 Gnb2l1 14694
Co-fractionation Mus musculus
13 Rps4x 20102
Co-fractionation Mus musculus
14 Rpl14 67115
Co-fractionation Mus musculus
15 Rps2 16898
Co-fractionation Mus musculus
16 Rpl10a 19896
Co-fractionation Mus musculus
17 Rpl32 19951
Co-fractionation Mus musculus
18 Rps23 66475
Co-fractionation Mus musculus
19 Rpl7a 27176
Co-fractionation Mus musculus
20 Rps18 20084
Co-fractionation Mus musculus
21 Rpl27 19942
Co-fractionation Mus musculus
22 Atxn1  
Proximity Label-MS Mus musculus
23 Rpl6 19988
Co-fractionation Mus musculus
24 Kctd13  
Affinity Capture-MS Mus musculus
25 Rpl9 20005
Co-fractionation Mus musculus
26 Rpl24 68193
Co-fractionation Mus musculus
27 Tpt1 22070
Co-fractionation Mus musculus
28 Rps11 27207
Co-fractionation Mus musculus
29 Rpl18a 76808
Co-fractionation Mus musculus
30 Rpl23 65019
Co-fractionation Mus musculus
31 Rps10 67097
Co-fractionation Mus musculus
32 Rpl17 319195
Co-fractionation Mus musculus
33 Rpl22 19934
Co-fractionation Mus musculus
34 Rpl4 67891
Co-fractionation Mus musculus
35 Fzr1  
Affinity Capture-MS Mus musculus
36 Rpl19 19921
Co-fractionation Mus musculus
37 Rps24 20088
Co-fractionation Mus musculus
38 Rpl26 19941
Co-fractionation Mus musculus
39 Rpl12 269261
Co-fractionation Mus musculus
40 Rpl18 19899
Co-fractionation Mus musculus
41 Rpl21 19933
Co-fractionation Mus musculus
42 Rps21 66481
Co-fractionation Mus musculus
43 Rpl7 19989
Co-fractionation Mus musculus
44 Rpl39 67248
Co-fractionation Mus musculus
45 Rps28 54127
Co-fractionation Mus musculus
46 Rpl38  
Co-fractionation Mus musculus
47 Rps15 20054
Co-fractionation Mus musculus
48 Rps5 20103
Co-fractionation Mus musculus
49 Rps7 20115
Co-fractionation Mus musculus
50 Rps3a1 20091
Co-fractionation Mus musculus
51 Rpl36a 19982
Co-fractionation Mus musculus
52 Npm1 18148
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
53 Rps13 68052
Co-fractionation Mus musculus
54 Rpl13 270106
Co-fractionation Mus musculus
55 Rplp0 11837
Co-fractionation Mus musculus
56 Rpl23a 268449
Co-fractionation Mus musculus
57 Rps14 20044
Co-fractionation Mus musculus
58 Rps17 20068
Co-fractionation Mus musculus
59 Rpl30 19946
Co-fractionation Mus musculus
60 Eed  
Affinity Capture-MS Mus musculus
61 Rpl15 66480
Co-fractionation Mus musculus
62 Rpl3 27367
Co-fractionation Mus musculus
63 Atg16l1  
Affinity Capture-MS Mus musculus
64 Rps16 20055
Co-fractionation Mus musculus
65 Fancd2  
Affinity Capture-MS Mus musculus
66 Rpl8 26961
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here