Gene description for Dhx9
Gene name DEAH (Asp-Glu-Ala-His) box polypeptide 9
Gene symbol Dhx9
Other names/aliases AI326842
Ddx9
HEL-5
NDHII
RHA
mHEL-5
Species Mus musculus
 Database cross references - Dhx9
ExoCarta ExoCarta_13211
Vesiclepedia VP_13211
Entrez Gene 13211
UniProt O70133  
 Dhx9 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dhx9
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISO
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISS
    RNA polymerase II complex binding GO:0000993 ISO
    regulatory region RNA binding GO:0001069 IDA
    regulatory region RNA binding GO:0001069 ISO
    DNA binding GO:0003677 ISS
    DNA helicase activity GO:0003678 ISO
    DNA helicase activity GO:0003678 ISS
    DNA replication origin binding GO:0003688 ISO
    DNA replication origin binding GO:0003688 ISS
    DNA clamp loader activity GO:0003689 IEA
    double-stranded DNA binding GO:0003690 ISO
    double-stranded DNA binding GO:0003690 ISS
    single-stranded DNA binding GO:0003697 ISO
    single-stranded DNA binding GO:0003697 ISS
    transcription coregulator activity GO:0003712 ISO
    transcription coactivator activity GO:0003713 ISO
    transcription coactivator activity GO:0003713 ISS
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 ISO
    RNA binding GO:0003723 ISS
    RNA helicase activity GO:0003724 IBA
    RNA helicase activity GO:0003724 ISO
    RNA helicase activity GO:0003724 ISS
    double-stranded RNA binding GO:0003725 ISO
    double-stranded RNA binding GO:0003725 ISS
    single-stranded RNA binding GO:0003727 ISO
    single-stranded RNA binding GO:0003727 ISS
    mRNA binding GO:0003729 ISO
    mRNA binding GO:0003729 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 ISO
    ATP hydrolysis activity GO:0016887 ISS
    ribonucleoside triphosphate phosphatase activity GO:0017111 ISS
    chromatin DNA binding GO:0031490 ISO
    chromatin DNA binding GO:0031490 ISS
    3'-5' DNA/RNA helicase activity GO:0033679 ISO
    3'-5' DNA/RNA helicase activity GO:0033679 ISS
    3'-5' RNA helicase activity GO:0034458 ISO
    3'-5' RNA helicase activity GO:0034458 ISS
    siRNA binding GO:0035197 ISO
    RNA stem-loop binding GO:0035613 ISO
    RNA stem-loop binding GO:0035613 ISS
    ribosome binding GO:0043022 ISO
    3'-5' DNA helicase activity GO:0043138 IBA
    3'-5' DNA helicase activity GO:0043138 ISO
    3'-5' DNA helicase activity GO:0043138 ISS
    triplex DNA binding GO:0045142 ISO
    triplex DNA binding GO:0045142 ISS
    metal ion binding GO:0046872 IEA
    nucleoside triphosphate diphosphatase activity GO:0047429 ISO
    nucleoside triphosphate diphosphatase activity GO:0047429 ISS
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISO
    importin-alpha family protein binding GO:0061676 ISO
    importin-alpha family protein binding GO:0061676 ISS
    cohesin loader activity GO:0061775 IEA
    RNA polymerase binding GO:0070063 ISO
    RNA polymerase binding GO:0070063 ISS
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    RISC complex binding GO:1905172 ISO
    RISC complex binding GO:1905172 ISS
    single-stranded 3'-5' DNA helicase activity GO:1990518 ISO
    single-stranded 3'-5' DNA helicase activity GO:1990518 ISS
    sequence-specific mRNA binding GO:1990825 ISO
    sequence-specific mRNA binding GO:1990825 ISS
    promoter-specific chromatin binding GO:1990841 ISO
    promoter-specific chromatin binding GO:1990841 ISS
Biological Process
    alternative mRNA splicing, via spliceosome GO:0000380 ISO
    alternative mRNA splicing, via spliceosome GO:0000380 ISS
    chromatin remodeling GO:0006338 IEA
    DNA-templated transcription termination GO:0006353 IEA
    regulation of transcription by RNA polymerase II GO:0006357 ISO
    circadian rhythm GO:0007623 IMP
    RNA secondary structure unwinding GO:0010501 ISO
    RNA secondary structure unwinding GO:0010501 ISS
    DNA duplex unwinding GO:0032508 ISO
    DNA duplex unwinding GO:0032508 ISS
    positive regulation of interferon-alpha production GO:0032727 ISO
    positive regulation of interferon-alpha production GO:0032727 ISS
    positive regulation of interferon-beta production GO:0032728 ISO
    positive regulation of interferon-beta production GO:0032728 ISS
    positive regulation of interleukin-18 production GO:0032741 IMP
    positive regulation of interleukin-6 production GO:0032755 ISO
    positive regulation of interleukin-6 production GO:0032755 ISS
    positive regulation of tumor necrosis factor production GO:0032760 ISO
    positive regulation of tumor necrosis factor production GO:0032760 ISS
    cellular response to heat GO:0034605 IDA
    miRNA-mediated post-transcriptional gene silencing GO:0035195 ISO
    miRNA-mediated post-transcriptional gene silencing GO:0035195 ISS
    DNA-templated viral transcription GO:0039695 ISO
    G-quadruplex DNA unwinding GO:0044806 ISO
    G-quadruplex DNA unwinding GO:0044806 ISS
    innate immune response GO:0045087 IEA
    positive regulation of innate immune response GO:0045089 IMP
    positive regulation of DNA repair GO:0045739 ISO
    positive regulation of DNA repair GO:0045739 ISS
    positive regulation of DNA replication GO:0045740 ISO
    positive regulation of DNA replication GO:0045740 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    positive regulation of RNA export from nucleus GO:0046833 ISO
    positive regulation of RNA export from nucleus GO:0046833 ISS
    positive regulation of fibroblast proliferation GO:0048146 ISO
    positive regulation of fibroblast proliferation GO:0048146 ISS
    positive regulation of viral transcription GO:0050434 ISO
    regulation of mRNA processing GO:0050684 IBA
    regulation of mRNA processing GO:0050684 ISO
    regulation of mRNA processing GO:0050684 ISS
    regulation of defense response to virus by host GO:0050691 IMP
    positive regulation of inflammatory response GO:0050729 IMP
    mRNA transport GO:0051028 IEA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISS
    positive regulation of response to cytokine stimulus GO:0060760 ISO
    positive regulation of response to cytokine stimulus GO:0060760 ISS
    pyroptotic inflammatory response GO:0070269 IMP
    RISC complex assembly GO:0070922 ISO
    RISC complex assembly GO:0070922 ISS
    CRD-mediated mRNA stabilization GO:0070934 ISO
    cellular response to tumor necrosis factor GO:0071356 ISO
    cellular response to tumor necrosis factor GO:0071356 ISS
    cellular response to exogenous dsRNA GO:0071360 ISO
    cellular response to exogenous dsRNA GO:0071360 ISS
    chromatin looping GO:0140588 IEA
    negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900152 ISO
    protein localization to cytoplasmic stress granule GO:1903608 ISO
    positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity GO:2000373 ISO
    positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity GO:2000373 ISS
    regulation of cytoplasmic translation GO:2000765 ISO
    regulation of cytoplasmic translation GO:2000765 ISS
    positive regulation of cytoplasmic translation GO:2000767 ISO
    positive regulation of cytoplasmic translation GO:2000767 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 ISS
    perichromatin fibrils GO:0005726 ISO
    perichromatin fibrils GO:0005726 ISS
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 ISO
    actin cytoskeleton GO:0015629 ISO
    actin cytoskeleton GO:0015629 ISS
    RISC complex GO:0016442 ISO
    RISC complex GO:0016442 ISS
    nuclear body GO:0016604 ISO
    nuclear body GO:0016604 ISS
    protein-containing complex GO:0032991 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 ISS
    RISC-loading complex GO:0070578 ISO
    RISC-loading complex GO:0070578 ISS
    CRD-mediated mRNA stability complex GO:0070937 ISO
    CRD-mediated mRNA stability complex GO:0070937 ISS
    nuclear stress granule GO:0097165 ISO
    nuclear stress granule GO:0097165 ISS
    ribonucleoprotein complex GO:1990904 IBA
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Dhx9 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dhx9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Elf5  
Affinity Capture-MS Mus musculus
3 MKRN1 23608
Affinity Capture-MS Homo sapiens
4 Rpl13a 22121
Co-fractionation Mus musculus
5 Sarnp 66118
Co-fractionation Mus musculus
6 Stau1  
Co-fractionation Mus musculus
7 Rpl26 19941
Co-fractionation Mus musculus
8 Pou5f1  
Affinity Capture-MS Mus musculus
9 Rps4x 20102
Co-fractionation Mus musculus
10 Syncrip 56403
Co-fractionation Mus musculus
11 Rps8 20116
Co-fractionation Mus musculus
12 Ybx1 22608
Co-fractionation Mus musculus
13 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
14 Rpl6 19988
Co-fractionation Mus musculus
15 Kctd13  
Affinity Capture-MS Mus musculus
16 Tfe3  
Affinity Capture-MS Mus musculus
17 Cul3  
Affinity Capture-MS Mus musculus
18 Atg16l1  
Affinity Capture-MS Mus musculus
19 Rps6 20104
Co-fractionation Mus musculus
20 Ncl 17975
Co-fractionation Mus musculus
21 Mecp2  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
22 Poldip3  
Co-fractionation Mus musculus
23 Rock2  
Co-fractionation Mus musculus
24 Rps9 76846
Co-fractionation Mus musculus
25 Ina  
Co-fractionation Mus musculus
26 Sra1  
Co-fractionation Mus musculus
27 Nanog  
Affinity Capture-MS Mus musculus
28 Rps3a1 20091
Co-fractionation Mus musculus
29 Npm1 18148
Affinity Capture-MS Mus musculus
30 Zbtb7b  
Affinity Capture-MS Mus musculus
31 Rplp0 11837
Co-fractionation Mus musculus
32 Eed  
Affinity Capture-MS Mus musculus
33 Fmr1  
Co-fractionation Mus musculus
34 Rac1 19353
Co-fractionation Mus musculus
35 Fancd2  
Affinity Capture-MS Mus musculus
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