Gene description for Ago1
Gene name argonaute RISC catalytic subunit 1
Gene symbol Ago1
Other names/aliases Eif2c1
Species Mus musculus
 Database cross references - Ago1
ExoCarta ExoCarta_236511
Vesiclepedia VP_236511
Entrez Gene 236511
UniProt Q8CJG1  
 Ago1 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
Neural stem cells 25242146    
 Gene ontology annotations for Ago1
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IMP
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    RNA polymerase II complex binding GO:0000993 ISO
    core promoter sequence-specific DNA binding GO:0001046 ISO
    RNA binding GO:0003723 IDA
    RNA binding GO:0003723 ISO
    double-stranded RNA binding GO:0003725 ISO
    single-stranded RNA binding GO:0003727 IBA
    single-stranded RNA binding GO:0003727 ISO
    protein binding GO:0005515 IPI
    miRNA binding GO:0035198 IBA
    miRNA binding GO:0035198 IDA
    miRNA binding GO:0035198 ISO
Biological Process
    nuclear-transcribed mRNA catabolic process GO:0000956 ISO
    nuclear-transcribed mRNA catabolic process GO:0000956 ISS
    RNA secondary structure unwinding GO:0010501 ISO
    miRNA metabolic process GO:0010586 IDA
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of gene expression GO:0010628 IMP
    negative regulation of angiogenesis GO:0016525 ISO
    pre-miRNA processing GO:0031054 IBA
    pre-miRNA processing GO:0031054 ISO
    regulatory ncRNA-mediated post-transcriptional gene silencing GO:0035194 IBA
    miRNA processing GO:0035196 ISO
    miRNA-mediated gene silencing by inhibition of translation GO:0035278 ISO
    miRNA-mediated gene silencing by inhibition of translation GO:0035278 ISS
    regulation of mRNA stability GO:0043488 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    RISC complex assembly GO:0070922 ISO
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 IMP
Subcellular Localization
    P-body GO:0000932 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    RISC complex GO:0016442 IBA
    RISC complex GO:0016442 IDA
    RISC complex GO:0016442 ISO
    RISC complex GO:0016442 ISS
    cytoplasmic ribonucleoprotein granule GO:0036464 IBA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    RISC-loading complex GO:0070578 ISO
 Experiment description of studies that identified Ago1 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 262
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|HSP90|CD63|CD9|
Enriched markers
Negative markers
Y
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Microarray
 Protein-protein interactions for Ago1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Fxr1 14359
Affinity Capture-MS Mus musculus
4 Hnrnpu 51810
Co-fractionation Mus musculus
5 Fmr1  
Affinity Capture-MS Mus musculus
6 Eprs 107508
Co-fractionation Mus musculus
7 Trim32  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
8 Hspa8 15481
Co-fractionation Mus musculus
9 Pex5l  
Affinity Capture-MS Mus musculus
10 Tdrd1  
Affinity Capture-Western Mus musculus
11 Rps4x 20102
Co-fractionation Mus musculus
12 Sra1  
Co-fractionation Mus musculus
13 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here