Gene description for Rps20
Gene name ribosomal protein S20
Gene symbol Rps20
Other names/aliases 4632426K06Rik
Dsk4
Species Mus musculus
 Database cross references - Rps20
ExoCarta ExoCarta_67427
Vesiclepedia VP_67427
Entrez Gene 67427
UniProt P60867  
 Rps20 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rps20
Molecular Function
    RNA binding GO:0003723 IEA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    MDM2/MDM4 family protein binding GO:0097371 IEA
    MDM2/MDM4 family protein binding GO:0097371 ISO
    ubiquitin ligase inhibitor activity GO:1990948 IEA
    ubiquitin ligase inhibitor activity GO:1990948 ISO
Biological Process
    cytoplasmic translation GO:0002181 NAS
    positive regulation of signal transduction by p53 class mediator GO:1901798 IEA
    positive regulation of signal transduction by p53 class mediator GO:1901798 ISO
Subcellular Localization
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    cytosolic ribosome GO:0022626 ISO
    cytosolic small ribosomal subunit GO:0022627 IBA
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytosolic small ribosomal subunit GO:0022627 ISO
    cytosolic small ribosomal subunit GO:0022627 NAS
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    postsynapse GO:0098794 NAS
 Experiment description of studies that identified Rps20 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rps20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Rps3 27050
Co-fractionation Mus musculus
3 Rpl11 67025
Co-fractionation Mus musculus
4 Rps18 20084
Co-fractionation Mus musculus
5 Eif3a 13669
Co-fractionation Mus musculus
6 Rps28 54127
Co-fractionation Mus musculus
7 Rps10 67097
Co-fractionation Mus musculus
8 Rplp2 67186
Co-fractionation Mus musculus
9 Rps4x 20102
Co-fractionation Mus musculus
10 Rpl14 67115
Co-fractionation Mus musculus
11 Rps2 16898
Co-fractionation Mus musculus
12 Rpl10a 19896
Co-fractionation Mus musculus
13 Rps8 20116
Co-fractionation Mus musculus
14 Rps23 66475
Co-fractionation Mus musculus
15 Rpl7a 27176
Co-fractionation Mus musculus
16 Rpl3 27367
Co-fractionation Mus musculus
17 Rpl32 19951
Co-fractionation Mus musculus
18 Wibg  
Co-fractionation Mus musculus
19 Rpl6 19988
Co-fractionation Mus musculus
20 Kctd13  
Affinity Capture-MS Mus musculus
21 Rps19 20085
Co-fractionation Mus musculus
22 Pkm 18746
Co-fractionation Mus musculus
23 Tpt1 22070
Co-fractionation Mus musculus
24 Rps11 27207
Co-fractionation Mus musculus
25 Rpl18a 76808
Co-fractionation Mus musculus
26 Rps24 20088
Co-fractionation Mus musculus
27 Rpl13 270106
Co-fractionation Mus musculus
28 Rps7 20115
Co-fractionation Mus musculus
29 Rpl22 19934
Co-fractionation Mus musculus
30 Serbp1 66870
Co-fractionation Mus musculus
31 Tmem173  
Proximity Label-MS Mus musculus
32 Fzr1  
Affinity Capture-MS Mus musculus
33 Rpl19 19921
Co-fractionation Mus musculus
34 Rps6 20104
Co-fractionation Mus musculus
35 Rpl7 19989
Co-fractionation Mus musculus
36 Rpl18 19899
Co-fractionation Mus musculus
37 Rps9 76846
Co-fractionation Mus musculus
38 Rps21 66481
Co-fractionation Mus musculus
39 Rpl12 269261
Co-fractionation Mus musculus
40 Gnb2l1 14694
Co-fractionation Mus musculus
41 Rps5 20103
Co-fractionation Mus musculus
42 Rps3a1 20091
Co-fractionation Mus musculus
43 Rpl26 19941
Co-fractionation Mus musculus
44 Fancd2  
Affinity Capture-MS Mus musculus
45 Rplp0 11837
Co-fractionation Mus musculus
46 Rpl23a 268449
Co-fractionation Mus musculus
47 Rps14 20044
Co-fractionation Mus musculus
48 Rpl30 19946
Co-fractionation Mus musculus
49 Eed  
Affinity Capture-MS Mus musculus
50 Rps27 57294
Co-fractionation Mus musculus
51 Rpsa 16785
Co-fractionation Mus musculus
52 Gapdhs  
Co-fractionation Mus musculus
53 Eef1b2 55949
Co-fractionation Mus musculus
54 Tpi1 21991
Co-fractionation Mus musculus
55 Rpl4 67891
Co-fractionation Mus musculus
56 Hba 15121
Co-fractionation Mus musculus
57 Rpl8 26961
Co-fractionation Mus musculus
58 Rps12 20042
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here