Gene description for Gapdh
Gene name glyceraldehyde-3-phosphate dehydrogenase
Gene symbol Gapdh
Other names/aliases Gapd
Species Mus musculus
 Database cross references - Gapdh
ExoCarta ExoCarta_14433
Vesiclepedia VP_14433
Entrez Gene 14433
UniProt P16858  
 Gapdh identified in sEVs derived from the following tissue/cell type
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 36648227    
Macrophages 36648227    
Macrophages 36648227    
Macrophages 36648227    
Macrophages 36648227    
Mast cells 17486113    
Melanoma cells 30089911    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Microglia 16081791    
Mov neuroglial cells 15210972    
Neural stem cells 25242146    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gapdh
Molecular Function
    glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity GO:0004365 IBA
    glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity GO:0004365 IDA
    glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity GO:0004365 IEA
    glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity GO:0004365 IMP
    glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity GO:0004365 ISO
    glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity GO:0004365 ISS
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 ISO
    microtubule binding GO:0008017 ISS
    aspartic-type endopeptidase inhibitor activity GO:0019828 ISO
    enzyme binding GO:0019899 IPI
    peptidyl-cysteine S-nitrosylase activity GO:0035605 IEA
    peptidyl-cysteine S-nitrosylase activity GO:0035605 ISO
    peptidyl-cysteine S-nitrosylase activity GO:0035605 ISS
    identical protein binding GO:0042802 ISO
    NADP binding GO:0050661 IEA
    NAD binding GO:0051287 IEA
    disordered domain specific binding GO:0097718 ISO
Biological Process
    microtubule cytoskeleton organization GO:0000226 ISO
    microtubule cytoskeleton organization GO:0000226 ISS
    positive regulation of cytokine production GO:0001819 ISO
    glucose metabolic process GO:0006006 IEA
    gluconeogenesis GO:0006094 IDA
    gluconeogenesis GO:0006094 ISO
    glycolytic process GO:0006096 IBA
    glycolytic process GO:0006096 IEA
    glycolytic process GO:0006096 ISO
    regulation of translation GO:0006417 IEA
    apoptotic process GO:0006915 IEA
    nitric oxide mediated signal transduction GO:0007263 ISO
    multicellular organism development GO:0007275 IMP
    negative regulation of endopeptidase activity GO:0010951 ISO
    negative regulation of translation GO:0017148 IDA
    negative regulation of translation GO:0017148 ISO
    cAMP-mediated signaling GO:0019933 ISO
    killing of cells of another organism GO:0031640 ISO
    positive regulation of type I interferon production GO:0032481 ISO
    positive regulation of type I interferon production GO:0032481 ISS
    peptidyl-cysteine S-trans-nitrosylation GO:0035606 ISS
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISO
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISS
    innate immune response GO:0045087 IEA
    glyceraldehyde-3-phosphate biosynthetic process GO:0046166 ISO
    protein stabilization GO:0050821 ISO
    protein stabilization GO:0050821 ISS
    defense response to fungus GO:0050832 ISO
    neuron apoptotic process GO:0051402 ISO
    neuron apoptotic process GO:0051402 ISS
    killing by host of symbiont cells GO:0051873 ISO
    canonical glycolysis GO:0061621 IDA
    canonical glycolysis GO:0061621 IMP
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 ISO
    cellular response to type II interferon GO:0071346 ISO
    negative regulation of vascular associated smooth muscle cell apoptotic process GO:1905460 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 HDA
    lipid droplet GO:0005811 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    microtubule cytoskeleton GO:0015630 ISO
    microtubule cytoskeleton GO:0015630 ISS
    late endosome lumen GO:0031906 TAS
    nuclear membrane GO:0031965 ISO
    myelin sheath GO:0043209 HDA
    intracellular membrane-bounded organelle GO:0043231 ISO
    GAIT complex GO:0097452 IDA
    GAIT complex GO:0097452 ISO
    glutamatergic synapse GO:0098978 ISO
    postsynaptic density, intracellular component GO:0099092 ISO
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Gapdh in sEVs
1
Experiment ID 26
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
4
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 569
MISEV standards
✘ Biophysical techniques
✔
Hsp90|Gapdh
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36648227    
Organism Mus musculus
Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M."
Journal name mBio
Publication year 2023
Sample Macrophages
Sample name RAW264.2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
9
Experiment ID 570
MISEV standards
✘ Biophysical techniques
✔
Hsp90|Gapdh|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36648227    
Organism Mus musculus
Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M."
Journal name mBio
Publication year 2023
Sample Macrophages
Sample name RAW264.2 - E. coli infected
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
10
Experiment ID 571
MISEV standards
✘ Biophysical techniques
✔
Hsp90|Gapdh|Cd9|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36648227    
Organism Mus musculus
Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M."
Journal name mBio
Publication year 2023
Sample Macrophages
Sample name RAW264.2 - E. coli infected
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
11
Experiment ID 580
MISEV standards
✘ Biophysical techniques
✔
Hsp90|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36648227    
Organism Mus musculus
Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M."
Journal name mBio
Publication year 2023
Sample Macrophages
Sample name BMDM
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
12
Experiment ID 581
MISEV standards
✘ Biophysical techniques
✔
Hsp90|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36648227    
Organism Mus musculus
Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M."
Journal name mBio
Publication year 2023
Sample Macrophages
Sample name BMDM
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
13
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
14
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
15
Experiment ID 968
MISEV standards
✘ Biophysical techniques
✔
Tsg101|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Mus musculus
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
16
Experiment ID 1101
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 1102
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
18
Experiment ID 1103
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 1163
MISEV standards
✘ Biophysical techniques
✔
Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
20
Experiment ID 1164
MISEV standards
✘ Biophysical techniques
✔
Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name C57BL/6 bearing B16-F10 tumour
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
21
Experiment ID 1165
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
22
Experiment ID 1166
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
23
Experiment ID 1167
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
24
Experiment ID 1168
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
25
Experiment ID 1169
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name C57BL/6 bearing B16-F10 tumour
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
26
Experiment ID 1170
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name C57BL/6 bearing B16-F10 tumour
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
27
Experiment ID 1171
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name C57BL/6 bearing B16-F10 tumour
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
28
Experiment ID 1172
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name C57BL/6 bearing B16-F10 tumour
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
29
Experiment ID 1173
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
30
Experiment ID 1174
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
31
Experiment ID 1175
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
32
Experiment ID 1176
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
33
Experiment ID 1177
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
34
Experiment ID 1178
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
35
Experiment ID 1179
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
36
Experiment ID 1180
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
37
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
38
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
39
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
40
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
41
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
42
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gapdh
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Eprs 107508
Co-fractionation Mus musculus
3 Rpl13a 22121
Co-fractionation Mus musculus
4 Klhl40  
Affinity Capture-MS Mus musculus
5 Rps4x 20102
Co-fractionation Mus musculus
6 Syncrip 56403
Co-fractionation Mus musculus
7 Gapdh 14433
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
8 Rps8 20116
Co-fractionation Mus musculus
9 Ybx1 22608
Co-fractionation Mus musculus
10 Fmr1  
Co-fractionation Mus musculus
11 Kctd13  
Affinity Capture-MS Mus musculus
12 Thrb  
Affinity Capture-MS Mus musculus
13 Tfe3  
Affinity Capture-MS Mus musculus
14 Snap25 20614
Affinity Capture-MS Mus musculus
15 HSD17B10 3028
Affinity Capture-MS Homo sapiens
16 Siah1a  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
17 Kcnma1  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
18 Ywhae 22627
Affinity Capture-MS Mus musculus
19 Tmem173  
Proximity Label-MS Mus musculus
20 Ccar2  
Co-localization Mus musculus
Affinity Capture-Western Mus musculus
21 Usp25 30940
Affinity Capture-MS Mus musculus
22 Sra1  
Co-fractionation Mus musculus
23 Nanog  
Affinity Capture-MS Mus musculus
24 Rps3a1 20091
Co-fractionation Mus musculus
25 Rpl26 19941
Co-fractionation Mus musculus
26 Rplp0 11837
Co-fractionation Mus musculus
27 Eed  
Affinity Capture-MS Mus musculus
28 L1cam 16728
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
29 Fancd2  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
30 Lipc  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Gapdh is involved
PathwayEvidenceSource
Gluconeogenesis IEA Reactome
Glucose metabolism IEA Reactome
Glycolysis IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome





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