Gene description for Eef1a1
Gene name eukaryotic translation elongation factor 1 alpha 1
Gene symbol Eef1a1
Other names/aliases -
Species Mus musculus
 Database cross references - Eef1a1
ExoCarta ExoCarta_13627
Vesiclepedia VP_13627
Entrez Gene 13627
UniProt P10126  
 Eef1a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Dendritic cells 11390481    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Mov neuroglial cells 15210972    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eef1a1
Molecular Function
    tRNA binding GO:0000049 IEA
    tRNA binding GO:0000049 ISO
    mRNA binding GO:0003729 ISO
    translation elongation factor activity GO:0003746 IBA
    translation elongation factor activity GO:0003746 ISO
    translation elongation factor activity GO:0003746 ISS
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IDA
    GTP binding GO:0005525 IEA
    kinase activator activity GO:0019209 ISS
    kinase binding GO:0019900 ISO
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    identical protein binding GO:0042802 ISO
    molecular adaptor activity GO:0060090 IEA
    molecular adaptor activity GO:0060090 ISO
    heterocyclic compound binding GO:1901363 ISO
Biological Process
    translation GO:0006412 IBA
    translational elongation GO:0006414 IBA
    translational elongation GO:0006414 ISO
    translational elongation GO:0006414 ISS
    positive regulation by host of viral genome replication GO:0044829 IEA
    positive regulation by host of viral genome replication GO:0044829 ISO
    cellular response to epidermal growth factor stimulus GO:0071364 ISO
    cellular response to epidermal growth factor stimulus GO:0071364 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleolus GO:0005730 ISO
    nucleolus GO:0005730 ISS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    ribosome GO:0005840 ISO
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    cytosolic ribosome GO:0022626 IEA
    cytosolic ribosome GO:0022626 ISO
    cortical actin cytoskeleton GO:0030864 IEA
    cortical actin cytoskeleton GO:0030864 ISO
    ruffle membrane GO:0032587 IEA
    ruffle membrane GO:0032587 ISO
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Eef1a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 10
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|CD9|MHCII|MHCI|CD86|LAMP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QTOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors "Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S."
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
8
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
9
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
10
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
11
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eef1a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 EEF1G 1937
Affinity Capture-MS Homo sapiens
3 BBS9 27241
Affinity Capture-MS Homo sapiens
4 TOP2B 7155
Affinity Capture-MS Homo sapiens
5 Rps18 20084
Co-fractionation Mus musculus
6 GAK 2580
Affinity Capture-MS Homo sapiens
7 Rpl6 19988
Co-fractionation Mus musculus
8 Thrb  
Affinity Capture-MS Mus musculus
9 Rps24 20088
Co-fractionation Mus musculus
10 Mks1  
Affinity Capture-MS Mus musculus
11 ENOX1  
Affinity Capture-MS Homo sapiens
12 Ywhaz 22631
Affinity Capture-MS Mus musculus
13 VPS29 51699
Affinity Capture-MS Homo sapiens
14 Tmem173  
Proximity Label-MS Mus musculus
15 Rpl7 19989
Co-fractionation Mus musculus
16 DARS 1615
Affinity Capture-MS Homo sapiens
17 Unk  
Affinity Capture-RNA Mus musculus
18 VARS 7407
Affinity Capture-MS Homo sapiens
19 Rpl26 19941
Co-fractionation Mus musculus
20 Rpsa 16785
Co-fractionation Mus musculus
21 Mapt  
Affinity Capture-MS Mus musculus
22 Set 56086
Affinity Capture-MS Mus musculus
23 HERC2 8924
Affinity Capture-MS Homo sapiens
24 Lipc  
Affinity Capture-MS Mus musculus
25 PHB 5245
Affinity Capture-MS Homo sapiens
26 Rpl11 67025
Co-fractionation Mus musculus
27 Rpl31 114641
Co-fractionation Mus musculus
28 Rplp2 67186
Co-fractionation Mus musculus
29 Rps4x 20102
Co-fractionation Mus musculus
30 Rps21 66481
Co-fractionation Mus musculus
31 Rps23 66475
Co-fractionation Mus musculus
32 Rpl18 19899
Co-fractionation Mus musculus
33 Vars 22321
Co-fractionation Mus musculus
34 Dlg4  
Affinity Capture-MS Mus musculus
35 Tfe3  
Affinity Capture-MS Mus musculus
36 MARS 4141
Affinity Capture-MS Homo sapiens
37 Rps7 20115
Co-fractionation Mus musculus
38 MAPT  
Reconstituted Complex Homo sapiens
39 Rps5 20103
Co-fractionation Mus musculus
40 TRPM7 54822
Affinity Capture-MS Homo sapiens
41 Rplp0 11837
Co-fractionation Mus musculus
42 Rpl39 67248
Co-fractionation Mus musculus
43 IARS 3376
Affinity Capture-MS Homo sapiens
44 Gapdhs  
Co-fractionation Mus musculus
45 AIMP1 9255
Affinity Capture-MS Homo sapiens
46 Rps16 20055
Co-fractionation Mus musculus
47 Rps17 20068
Co-fractionation Mus musculus
48 CARS 833
Affinity Capture-MS Homo sapiens
49 Raver1  
Co-fractionation Mus musculus
50 Rps19 20085
Co-fractionation Mus musculus
51 BRF1  
Affinity Capture-MS Homo sapiens
52 Tubgcp2  
Co-fractionation Mus musculus
53 Vars2  
Co-fractionation Mus musculus
54 RGS12 6002
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
55 Eif2a  
Co-fractionation Mus musculus
56 Gnb2l1 14694
Co-fractionation Mus musculus
57 Rpl14 67115
Co-fractionation Mus musculus
58 Rps2 16898
Co-fractionation Mus musculus
59 Kcnma1  
Affinity Capture-MS Mus musculus
60 Rpl7a 27176
Co-fractionation Mus musculus
61 Rpl32 19951
Co-fractionation Mus musculus
62 Rps28 54127
Co-fractionation Mus musculus
63 Rpl3 27367
Co-fractionation Mus musculus
64 Tpt1 22070
Co-fractionation Mus musculus
65 EGFL7 51162
Affinity Capture-MS Homo sapiens
66 Rps11 27207
Co-fractionation Mus musculus
67 Rpl18a 76808
Co-fractionation Mus musculus
68 EEF1B2 1933
Affinity Capture-MS Homo sapiens
69 Ywhae 22627
Affinity Capture-MS Mus musculus
70 LARS 51520
Affinity Capture-MS Homo sapiens
71 Rpl19 19921
Co-fractionation Mus musculus
72 Traf6  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
73 Rpl38  
Co-fractionation Mus musculus
74 Rps3a1 20091
Co-fractionation Mus musculus
75 EPRS 2058
Affinity Capture-MS Homo sapiens
76 Rnf123  
Affinity Capture-MS Mus musculus
77 Rps14 20044
Co-fractionation Mus musculus
78 KTN1 3895
Affinity Capture-MS Homo sapiens
79 Eed  
Affinity Capture-MS Mus musculus
80 Fancd2  
Affinity Capture-MS Mus musculus
81 Ptpn4  
Co-fractionation Mus musculus
82 Rps27a 78294
Co-fractionation Mus musculus
83 Sdhaf2  
Co-fractionation Mus musculus
84 Rps25 75617
Co-fractionation Mus musculus
85 PHB2 11331
Affinity Capture-MS Homo sapiens
86 TTC1 7265
Affinity Capture-MS Homo sapiens
87 RRBP1 6238
Affinity Capture-MS Homo sapiens
88 SVIL 6840
Affinity Capture-MS Homo sapiens
89 SLC39A7 7922
Affinity Capture-MS Homo sapiens
90 Kctd13  
Affinity Capture-MS Mus musculus
91 ERLIN2 11160
Affinity Capture-MS Homo sapiens
92 Iqcb1  
Affinity Capture-MS Mus musculus
93 Herc2  
Affinity Capture-MS Mus musculus
94 SOAT1 6646
Affinity Capture-MS Homo sapiens
95 EEF1D 1936
Affinity Capture-MS Homo sapiens
96 SPAG1 6674
Affinity Capture-MS Homo sapiens
97 CEP135  
Affinity Capture-MS Homo sapiens
98 Rps15 20054
Co-fractionation Mus musculus
99 Sfpq 71514
Co-fractionation Mus musculus
100 Rps13 68052
Co-fractionation Mus musculus
101 Rpl23 65019
Co-fractionation Mus musculus
102 Rpl23a 268449
Co-fractionation Mus musculus
103 RARS 5917
Affinity Capture-MS Homo sapiens
104 Rpl15 66480
Co-fractionation Mus musculus
105 Nphp1  
Affinity Capture-MS Mus musculus
106 Rpl4 67891
Co-fractionation Mus musculus
107 Otx2  
Affinity Capture-MS Mus musculus
108 Rpl13 270106
Co-fractionation Mus musculus
109 Rpl8 26961
Co-fractionation Mus musculus
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