Gene description for Ube2o
Gene name ubiquitin-conjugating enzyme E2O
Gene symbol Ube2o
Other names/aliases 9630022H21
B230113M03Rik
E2-230K
mKIAA1734
Species Mus musculus
 Database cross references - Ube2o
ExoCarta ExoCarta_217342
Vesiclepedia VP_217342
Entrez Gene 217342
UniProt Q6ZPJ3  
 Ube2o identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ube2o
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 ISO
    ubiquitin-protein transferase activity GO:0004842 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ubiquitin protein ligase activity GO:0061630 ISO
    ubiquitin conjugating enzyme activity GO:0061631 IBA
    ubiquitin conjugating enzyme activity GO:0061631 ISO
Biological Process
    protein monoubiquitination GO:0006513 ISO
    protein monoubiquitination GO:0006513 ISS
    positive regulation of BMP signaling pathway GO:0030513 ISO
    positive regulation of BMP signaling pathway GO:0030513 ISS
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 ISO
    retrograde transport, endosome to Golgi GO:0042147 ISS
    protein K63-linked ubiquitination GO:0070534 ISO
    protein K63-linked ubiquitination GO:0070534 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IEA
    nuclear body GO:0016604 IEA
    nuclear body GO:0016604 ISO
 Experiment description of studies that identified Ube2o in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ube2o
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pafah1b3  
Co-fractionation Mus musculus
2 Rps7 20115
Co-fractionation Mus musculus
3 Fancd2  
Affinity Capture-MS Mus musculus
4 Ybx1 22608
Co-fractionation Mus musculus
5 CPNE2 221184
Two-hybrid Homo sapiens
6 Cyfip1 20430
Affinity Capture-MS Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
8 Smarca4  
Co-fractionation Mus musculus
9 Cpne1 266692
Co-fractionation Mus musculus
10 Cpne4  
Co-fractionation Mus musculus
11 CPNE4 131034
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
12 CPNE1 8904
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
13 Nbr1  
Two-hybrid Mus musculus
14 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here