Gene description for Ddx17
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 17
Gene symbol Ddx17
Other names/aliases 2610007K22Rik
A430025E01Rik
AI047725
C80929
Gm926
p72
Species Mus musculus
 Database cross references - Ddx17
ExoCarta ExoCarta_67040
Vesiclepedia VP_67040
Entrez Gene 67040
UniProt Q501J6  
 Ddx17 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ddx17
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    transcription coactivator activity GO:0003713 ISO
    RNA helicase activity GO:0003724 IBA
    RNA helicase activity GO:0003724 IEA
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    chromatin DNA binding GO:0031490 IDA
    cohesin loader activity GO:0061775 IEA
    lncRNA binding GO:0106222 IDA
    lncRNA binding GO:0106222 IPI
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    alternative mRNA splicing, via spliceosome GO:0000380 IBA
    alternative mRNA splicing, via spliceosome GO:0000380 IMP
    alternative mRNA splicing, via spliceosome GO:0000380 ISO
    alternative mRNA splicing, via spliceosome GO:0000380 ISS
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IDA
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 ISO
    epithelial to mesenchymal transition GO:0001837 ISO
    epithelial to mesenchymal transition GO:0001837 ISS
    immune system process GO:0002376 IEA
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 ISO
    regulation of transcription by RNA polymerase II GO:0006357 ISS
    rRNA processing GO:0006364 IEA
    gene expression GO:0010467 IMP
    miRNA metabolic process GO:0010586 ISO
    miRNA metabolic process GO:0010586 ISS
    estrogen receptor signaling pathway GO:0030520 ISO
    estrogen receptor signaling pathway GO:0030520 ISS
    androgen receptor signaling pathway GO:0030521 ISO
    androgen receptor signaling pathway GO:0030521 ISS
    regulatory ncRNA-mediated gene silencing GO:0031047 IEA
    myoblast differentiation GO:0045445 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    defense response to virus GO:0051607 IEA
    miRNA transcription GO:0061614 IMP
    chromatin looping GO:0140588 IEA
    regulation of skeletal muscle cell differentiation GO:2001014 ISO
    regulation of skeletal muscle cell differentiation GO:2001014 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 IEA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IEA
    nuclear speck GO:0016607 ISO
    ribonucleoprotein complex GO:1990904 IBA
 Experiment description of studies that identified Ddx17 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ddx17
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 Creb3l4  
Affinity Capture-MS Mus musculus
3 Prpf18  
Co-fractionation Mus musculus
4 D2Wsu81e  
Co-fractionation Mus musculus
5 Ctps 51797
Co-fractionation Mus musculus
6 Cebpz  
Co-fractionation Mus musculus
7 Trmt1  
Co-fractionation Mus musculus
8 Fus  
Co-fractionation Mus musculus
9 Prpf8 192159
Co-fractionation Mus musculus
10 Cdc5l 71702
Co-fractionation Mus musculus
11 Smu1  
Co-fractionation Mus musculus
12 Pwp2  
Co-fractionation Mus musculus
13 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
14 Kctd13  
Affinity Capture-MS Mus musculus
15 Ddx27  
Co-fractionation Mus musculus
16 Ttc4  
Co-fractionation Mus musculus
17 Cwc22  
Co-fractionation Mus musculus
18 Gnl3  
Co-fractionation Mus musculus
19 Ncl 17975
Co-fractionation Mus musculus
20 Nop14  
Co-fractionation Mus musculus
21 Tardbp 230908
Co-fractionation Mus musculus
22 Wdr43  
Co-fractionation Mus musculus
23 Lsg1  
Co-fractionation Mus musculus
24 2310036O22Rik  
Co-fractionation Mus musculus
25 Spen  
Co-fractionation Mus musculus
26 Tfip11  
Co-fractionation Mus musculus
27 Sdad1  
Co-fractionation Mus musculus
28 Ppwd1  
Co-fractionation Mus musculus
29 Zbtb7b  
Affinity Capture-MS Mus musculus
30 Fancd2  
Affinity Capture-MS Mus musculus
31 Hnrnpdl 50926
Co-fractionation Mus musculus
32 Ilf3 16201
Co-fractionation Mus musculus
33 Eed  
Affinity Capture-MS Mus musculus
34 Prpf40a  
Co-fractionation Mus musculus
35 Cactin  
Co-fractionation Mus musculus
36 Mapt  
Affinity Capture-MS Mus musculus
37 Tia1  
Affinity Capture-MS Mus musculus
38 Otx2  
Affinity Capture-MS Mus musculus
39 Aatf  
Co-fractionation Mus musculus
40 Paxbp1  
Co-fractionation Mus musculus
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