Gene description for MPHOSPH8
Gene name M-phase phosphoprotein 8
Gene symbol MPHOSPH8
Other names/aliases HSMPP8
TWA3
mpp8
Species Homo sapiens
 Database cross references - MPHOSPH8
ExoCarta ExoCarta_54737
Vesiclepedia VP_54737
Entrez Gene 54737
HGNC 29810
MIM 611626
UniProt Q99549  
 MPHOSPH8 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MPHOSPH8
Molecular Function
    chromatin binding GO:0003682 IBA
    chromatin binding GO:0003682 IDA
    protein binding GO:0005515 IPI
    methylated histone binding GO:0035064 IBA
    methylated histone binding GO:0035064 IDA
    histone reader activity GO:0140566 IDA
Biological Process
    negative regulation of gene expression via chromosomal CpG island methylation GO:0044027 IMP
    negative regulation of gene expression, epigenetic GO:0045814 IDA
    constitutive heterochromatin formation GO:0140719 IDA
    transposable element silencing by heterochromatin formation GO:0141005 IDA
    transposable element silencing by heterochromatin formation GO:0141005 IMP
Subcellular Localization
    nucleosome GO:0000786 IDA
    heterochromatin GO:0000792 IBA
    heterochromatin GO:0000792 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified MPHOSPH8 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MPHOSPH8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG16L1 55054
Affinity Capture-MS Homo sapiens
2 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
3 FAM208A  
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
4 MECP2 4204
Affinity Capture-MS Homo sapiens
5 FASN 2194
Negative Genetic Homo sapiens
6 ANKLE2 23141
Affinity Capture-MS Homo sapiens
7 RPS11 6205
Affinity Capture-MS Homo sapiens
8 NXF1 10482
Affinity Capture-RNA Homo sapiens
9 ILF3 3609
Affinity Capture-MS Homo sapiens
10 HECW1  
Affinity Capture-MS Homo sapiens
11 MRPS12  
Affinity Capture-MS Homo sapiens
12 FBXL6  
Affinity Capture-MS Homo sapiens
13 MTRNR2L1  
Two-hybrid Homo sapiens
14 TRRAP 8295
Affinity Capture-MS Homo sapiens
15 TBC1D4  
Affinity Capture-MS Homo sapiens
16 SH3BP4 23677
Two-hybrid Homo sapiens
17 DYNLRB1 83658
Affinity Capture-MS Homo sapiens
18 ZEB1  
Affinity Capture-Western Homo sapiens
19 ESAM 90952
Affinity Capture-MS Homo sapiens
20 BTF3 689
Affinity Capture-MS Homo sapiens
21 BRPF1  
Affinity Capture-MS Homo sapiens
22 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
23 SREBF1 6720
Negative Genetic Homo sapiens
24 PLA2G4D  
Cross-Linking-MS (XL-MS) Homo sapiens
25 SAFB 6294
Affinity Capture-MS Homo sapiens
26 HMGN5 79366
Affinity Capture-MS Homo sapiens
27 PHIP 55023
Affinity Capture-MS Homo sapiens
28 LRWD1  
Affinity Capture-MS Homo sapiens
29 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
30 NUMA1 4926
Affinity Capture-MS Homo sapiens
31 UTP23  
Affinity Capture-MS Homo sapiens
32 ZNF770 54989
Affinity Capture-MS Homo sapiens
33 ERH 2079
Affinity Capture-MS Homo sapiens
34 YIPF5 81555
Affinity Capture-MS Homo sapiens
35 SIRT1  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
36 FAM195A  
Affinity Capture-MS Homo sapiens
37 TSR3  
Affinity Capture-MS Homo sapiens
38 ZNF597  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 DNMT3A 1788
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
40 PPHLN1  
Affinity Capture-MS Homo sapiens
41 NOP2 4839
Affinity Capture-MS Homo sapiens
42 RANBP9 10048
Two-hybrid Homo sapiens
43 ERCC6  
Affinity Capture-MS Homo sapiens
44 Srsf1 110809
Affinity Capture-MS Mus musculus
45 RPL36 25873
Affinity Capture-MS Homo sapiens
46 ZNF638 27332
Affinity Capture-MS Homo sapiens
47 ESYT2 57488
Affinity Capture-MS Homo sapiens
48 MOV10 4343
Affinity Capture-RNA Homo sapiens
49 UBTF 7343
Affinity Capture-MS Homo sapiens
50 SAFB2 9667
Affinity Capture-MS Homo sapiens
51 DDX28  
Affinity Capture-MS Homo sapiens
52 NOLC1 9221
Cross-Linking-MS (XL-MS) Homo sapiens
53 MATR3 9782
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 FAM208B 54906
Affinity Capture-MS Homo sapiens
55 NCOA5  
Affinity Capture-MS Homo sapiens
56 MPHOSPH8 54737
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
57 TOP1 7150
Affinity Capture-MS Homo sapiens
58 IMP3 55272
Affinity Capture-MS Homo sapiens
59 ZNF121  
Affinity Capture-MS Homo sapiens
60 RPL24 6152
Affinity Capture-MS Homo sapiens
61 EHMT1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
62 MB21D1  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 ENO1 2023
Affinity Capture-RNA Homo sapiens
64 USMG5 84833
Affinity Capture-MS Homo sapiens
65 BMI1  
Affinity Capture-MS Homo sapiens
66 KHDRBS1 10657
Affinity Capture-MS Homo sapiens
67 ZNF845  
Affinity Capture-MS Homo sapiens
68 SSRP1 6749
Affinity Capture-MS Homo sapiens
69 HCFC1 3054
Affinity Capture-MS Homo sapiens
70 TMEM33 55161
Affinity Capture-MS Homo sapiens
71 HIST1H3A 8350
Protein-peptide Homo sapiens
72 RPP25L  
Affinity Capture-MS Homo sapiens
73 MRPL18 29074
Affinity Capture-MS Homo sapiens
74 DAXX  
Affinity Capture-MS Homo sapiens
75 SPIRE2  
Affinity Capture-MS Homo sapiens
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