Gene description for Hspe1
Gene name heat shock protein 1 (chaperonin 10)
Gene symbol Hspe1
Other names/aliases 10kDa
Hsp10
mt-cpn10
Species Mus musculus
 Database cross references - Hspe1
ExoCarta ExoCarta_15528
Vesiclepedia VP_15528
Entrez Gene 15528
UniProt Q64433  
 Hspe1 identified in sEVs derived from the following tissue/cell type
Melanoma cells 34957415    
Melanoma cells 34957415    
 Gene ontology annotations for Hspe1
Molecular Function
    ATP binding GO:0005524 IEA
    protein folding chaperone GO:0044183 IEA
    metal ion binding GO:0046872 IBA
    unfolded protein binding GO:0051082 IBA
    protein-folding chaperone binding GO:0051087 IBA
    protein-folding chaperone binding GO:0051087 ISO
    protein-folding chaperone binding GO:0051087 ISS
Biological Process
    protein folding GO:0006457 IEA
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    mitochondrial matrix GO:0005759 IBA
    mitochondrial matrix GO:0005759 IEA
    mitochondrial matrix GO:0005759 ISO
 Experiment description of studies that identified Hspe1 in sEVs
1
Experiment ID 1117
MISEV standards
Biophysical techniques
Cd81|Icam1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1118
MISEV standards
Biophysical techniques
Cd81|Icam1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hspe1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ppif  
Cross-Linking-MS (XL-MS) Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 D10Jhu81e  
Cross-Linking-MS (XL-MS) Mus musculus
5 Hspd1 15510
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
6 Taldo1 21351
Co-fractionation Mus musculus
7 Tfe3  
Affinity Capture-MS Mus musculus
8 Hspe1 15528
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
9 Atg16l1  
Affinity Capture-MS Mus musculus
10 Unk  
Affinity Capture-RNA Mus musculus
11 Fdxr  
Co-fractionation Mus musculus
12 Thop1  
Co-fractionation Mus musculus
View the network image/svg+xml



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