Gene description for Sdha
Gene name succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
Gene symbol Sdha
Other names/aliases 1500032O14Rik
2310034D06Rik
4921513A11
C81073
FP
SDH2
SDHF
Species Mus musculus
 Database cross references - Sdha
ExoCarta ExoCarta_66945
Vesiclepedia VP_66945
Entrez Gene 66945
UniProt Q8K2B3  
 Sdha identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Sdha
Molecular Function
    succinate dehydrogenase activity GO:0000104 ISO
    succinate dehydrogenase (quinone) activity GO:0008177 IBA
    succinate dehydrogenase (quinone) activity GO:0008177 ISO
    succinate dehydrogenase (quinone) activity GO:0008177 ISS
    succinate dehydrogenase (quinone) activity GO:0008177 ISS
    electron transfer activity GO:0009055 IBA
    flavin adenine dinucleotide binding GO:0050660 IBA
    flavin adenine dinucleotide binding GO:0050660 IEA
Biological Process
    tricarboxylic acid cycle GO:0006099 IEA
    tricarboxylic acid cycle GO:0006099 NAS
    succinate metabolic process GO:0006105 ISO
    succinate metabolic process GO:0006105 ISS
    mitochondrial electron transport, succinate to ubiquinone GO:0006121 IBA
    mitochondrial electron transport, succinate to ubiquinone GO:0006121 NAS
    nervous system development GO:0007399 IEA
    nervous system development GO:0007399 ISO
    respiratory electron transport chain GO:0022904 ISO
    respiratory electron transport chain GO:0022904 ISS
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
Subcellular Localization
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial inner membrane GO:0005743 ISS
    mitochondrial inner membrane GO:0005743 NAS
    myelin sheath GO:0043209 HDA
    respiratory chain complex II (succinate dehydrogenase) GO:0045273 IBA
    respiratory chain complex II (succinate dehydrogenase) GO:0045273 IDA
    respiratory chain complex II (succinate dehydrogenase) GO:0045273 ISS
 Experiment description of studies that identified Sdha in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Sdha
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Got2 14719
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
2 Idh2 269951
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
3 Cox4i1 12857
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
4 Cs 12974
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
5 Atp5j  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
6 Sdhaf2  
Co-fractionation Mus musculus
7 Hspd1 15510
Co-fractionation Mus musculus
8 Atg16l1  
Affinity Capture-MS Mus musculus
9 Suclg1  
Co-fractionation Mus musculus
10 Cycs 13063
Co-fractionation Mus musculus
11 Aco2 11429
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
12 Atp5e  
Co-fractionation Mus musculus
13 Uqcr10  
Co-fractionation Mus musculus
14 Ndufa12  
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
15 Pdhb 68263
Co-fractionation Mus musculus
16 Usmg5  
Co-fractionation Mus musculus
17 Kctd13  
Affinity Capture-MS Mus musculus
18 Etfa 110842
Cross-Linking-MS (XL-MS) Mus musculus
19 Ddt  
Co-fractionation Mus musculus
20 ATP8  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
21 Cul4a  
Co-fractionation Mus musculus
22 HSD17B10 3028
Affinity Capture-MS Homo sapiens
23 Cox5a  
Co-fractionation Mus musculus
24 Sucla2  
Co-fractionation Mus musculus
25 Nnt 18115
Cross-Linking-MS (XL-MS) Mus musculus
26 Ncoa4  
Cross-Linking-MS (XL-MS) Mus musculus
27 Atp5o 28080
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
28 Atp5k  
Co-fractionation Mus musculus
29 Ogdh 18293
Co-fractionation Mus musculus
30 Atp5j2  
Co-fractionation Mus musculus
31 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
32 Fh1 14194
Co-fractionation Mus musculus
33 Sdha 66945
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
34 Atp5h 71679
Cross-Linking-MS (XL-MS) Mus musculus
35 Lyrm4  
Co-fractionation Mus musculus
36 Atp5b 11947
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
37 Fxn  
Co-fractionation Mus musculus
38 Csl  
Cross-Linking-MS (XL-MS) Mus musculus
39 Sdhb 67680
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
40 Atp5f1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
41 Vdac3 22335
Co-fractionation Mus musculus
42 Uqcrc1  
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
43 Hspa9 15526
Co-fractionation Mus musculus
44 Mapt  
Affinity Capture-MS Mus musculus
45 Dld 13382
Co-fractionation Mus musculus
46 Uqcrc2 67003
Co-fractionation Mus musculus
47 Idh3g  
Co-fractionation Mus musculus
48 Atp5c1  
Cross-Linking-MS (XL-MS) Mus musculus
49 Fancd2  
Affinity Capture-MS Mus musculus
50 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
51 Uqcrfs1 66694
Co-fractionation Mus musculus
52 Dlat  
Co-fractionation Mus musculus
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