Gene description for Atp5h
Gene name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit D
Gene symbol Atp5h
Other names/aliases 0610009D10Rik
Species Mus musculus
 Database cross references - Atp5h
ExoCarta ExoCarta_71679
Vesiclepedia VP_71679
Entrez Gene 71679
UniProt Q9DCX2  
 Atp5h identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Atp5h
Molecular Function
    protein binding GO:0005515 IPI
    protein-containing complex binding GO:0044877 ISO
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IBA
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IEA
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 ISO
Biological Process
    proton motive force-driven ATP synthesis GO:0015986 IBA
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 IEA
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 ISO
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial inner membrane GO:0005743 ISO
    myelin sheath GO:0043209 HDA
    proton-transporting ATP synthase complex GO:0045259 ISO
    proton-transporting ATP synthase complex GO:0045259 ISS
    proton-transporting ATP synthase complex, coupling factor F(o) GO:0045263 IEA
    proton-transporting ATP synthase complex, coupling factor F(o) GO:0045263 ISO
 Experiment description of studies that identified Atp5h in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Atp5h
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Got2 14719
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
2 Idh2 269951
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
3 Atp5h 71679
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
4 SNCA 6622
Affinity Capture-MS Homo sapiens
5 Atp5j  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
6 Etfb 110826
Cross-Linking-MS (XL-MS) Mus musculus
7 Hadha  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
8 Hadh  
Cross-Linking-MS (XL-MS) Mus musculus
9 Ndufa12  
Co-fractionation Mus musculus
10 Acadvl 11370
Cross-Linking-MS (XL-MS) Mus musculus
11 Kctd13  
Affinity Capture-MS Mus musculus
12 Rbm14  
Cross-Linking-MS (XL-MS) Mus musculus
13 Etfa 110842
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
14 Ech1  
Cross-Linking-MS (XL-MS) Mus musculus
15 ATP8  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
16 Aco2 11429
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
17 Atp5b 11947
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
18 Csl  
Cross-Linking-MS (XL-MS) Mus musculus
19 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
20 Fh1 14194
Cross-Linking-MS (XL-MS) Mus musculus
21 Sdha 66945
Cross-Linking-MS (XL-MS) Mus musculus
22 Acadm 11364
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
23 Cs 12974
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
24 Hadhb  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
25 Suclg1  
Cross-Linking-MS (XL-MS) Mus musculus
26 Acat1 110446
Cross-Linking-MS (XL-MS) Mus musculus
27 Abcd2  
Cross-Linking-MS (XL-MS) Mus musculus
28 Atp5f1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
29 Dld 13382
Cross-Linking-MS (XL-MS) Mus musculus
30 Ndufb6  
Co-fractionation Mus musculus
31 Fancd2  
Affinity Capture-MS Mus musculus
32 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
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