Gene description for Cs
Gene name citrate synthase
Gene symbol Cs
Other names/aliases 2610511A05Rik
9030605P22Rik
Ahl4
BB234005
Cis
Species Mus musculus
 Database cross references - Cs
ExoCarta ExoCarta_12974
Vesiclepedia VP_12974
Entrez Gene 12974
UniProt Q9CZU6  
 Cs identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cs
Molecular Function
    citrate (Si)-synthase activity GO:0004108 IBA
    citrate (Si)-synthase activity GO:0004108 IDA
    citrate (Si)-synthase activity GO:0004108 IGI
    citrate (Si)-synthase activity GO:0004108 ISO
    citrate (Si)-synthase activity GO:0004108 ISS
    identical protein binding GO:0042802 ISS
    acyltransferase activity, acyl groups converted into alkyl on transfer GO:0046912 IDA
Biological Process
    carbohydrate metabolic process GO:0005975 IBA
    carbohydrate metabolic process GO:0005975 ISO
    carbohydrate metabolic process GO:0005975 ISS
    acetyl-CoA metabolic process GO:0006084 ISO
    tricarboxylic acid cycle GO:0006099 IBA
    tricarboxylic acid cycle GO:0006099 IEA
    tricarboxylic acid cycle GO:0006099 ISO
    citrate metabolic process GO:0006101 IEA
    citrate metabolic process GO:0006101 ISO
    oxaloacetate metabolic process GO:0006107 ISO
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 IBA
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 ISO
    mitochondrial matrix GO:0005759 ISS
 Experiment description of studies that identified Cs in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cs
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Got2 14719
Cross-Linking-MS (XL-MS) Mus musculus
2 Idh2 269951
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
3 Atp5h 71679
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
4 Slc25a3 18674
Cross-Linking-MS (XL-MS) Mus musculus
5 Atp5j  
Cross-Linking-MS (XL-MS) Mus musculus
6 D10Jhu81e  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
7 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
8 Cisd1  
Co-fractionation Mus musculus
9 Aco2 11429
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
10 Uqcrc1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
11 Kctd13  
Affinity Capture-MS Mus musculus
12 Etfa 110842
Cross-Linking-MS (XL-MS) Mus musculus
13 Pdha1  
Co-fractionation Mus musculus
14 HSD17B10 3028
Affinity Capture-MS Homo sapiens
15 Dld 13382
Co-fractionation Mus musculus
16 Sucla2  
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
17 Nnt 18115
Cross-Linking-MS (XL-MS) Mus musculus
18 Atp5o 28080
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
19 Atp5j2  
Co-fractionation Mus musculus
20 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
21 MAPT  
Reconstituted Complex Homo sapiens
22 Sdha 66945
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
23 Acadm 11364
Cross-Linking-MS (XL-MS) Mus musculus
24 Fh1 14194
Cross-Linking-MS (XL-MS) Mus musculus
25 Cs 12974
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
26 Atp5b 11947
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
27 Suclg1  
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
28 Acat1 110446
Cross-Linking-MS (XL-MS) Mus musculus
29 Csl  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
30 Atp5f1  
Cross-Linking-MS (XL-MS) Mus musculus
31 Ndufa12  
Co-fractionation Mus musculus
32 Uqcrc2 67003
Co-fractionation Mus musculus
33 Atg16l1  
Affinity Capture-MS Mus musculus
34 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here