Gene description for Atp5o
Gene name ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
Gene symbol Atp5o
Other names/aliases ATPO
D12Wsu28e
OSCP
Species Mus musculus
 Database cross references - Atp5o
ExoCarta ExoCarta_28080
Vesiclepedia VP_28080
Entrez Gene 28080
UniProt Q9DB20  
 Atp5o identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Melanoma cells 34957415    
Melanoma cells 34957415    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Atp5o
Molecular Function
    ATP hydrolysis activity GO:0016887 ISO
    protein-containing complex binding GO:0044877 ISO
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IBA
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IEA
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 ISO
    estradiol binding GO:1903924 ISO
Biological Process
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 IBA
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 IEA
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 ISO
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 IEA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 ISO
    myelin sheath GO:0043209 HDA
    proton-transporting ATP synthase complex GO:0045259 ISO
    proton-transporting ATP synthase complex GO:0045259 ISS
    proton-transporting ATP synthase complex, coupling factor F(o) GO:0045263 ISO
 Experiment description of studies that identified Atp5o in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1117
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1118
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Atp5o
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Slc25a3 18674
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
2 Ppif  
Cross-Linking-MS (XL-MS) Mus musculus
3 Nphs2  
Affinity Capture-MS Mus musculus
4 Cox4i1 12857
Cross-Linking-MS (XL-MS) Mus musculus
5 Idh2 269951
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
6 Got2 14719
Cross-Linking-MS (XL-MS) Mus musculus
7 Atp5j  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
8 Hadha  
Cross-Linking-MS (XL-MS) Mus musculus
9 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
10 Ttn  
Cross-Linking-MS (XL-MS) Mus musculus
11 Aco2 11429
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
12 Atp5f1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
13 Kctd13  
Affinity Capture-MS Mus musculus
14 Uqcrc1  
Cross-Linking-MS (XL-MS) Mus musculus
15 Htt  
Affinity Capture-MS Mus musculus
16 Decr1  
Cross-Linking-MS (XL-MS) Mus musculus
17 Atp5b 11947
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
18 Atp5o 28080
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
19 Csl  
Cross-Linking-MS (XL-MS) Mus musculus
20 Stau1  
Co-fractionation Mus musculus
21 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
22 Fh1 14194
Cross-Linking-MS (XL-MS) Mus musculus
23 Sdha 66945
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
24 Acadm 11364
Cross-Linking-MS (XL-MS) Mus musculus
25 Cs 12974
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
26 Unk  
Affinity Capture-RNA Mus musculus
27 Cox5b 12859
Cross-Linking-MS (XL-MS) Mus musculus
28 Suclg1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
29 Abcd2  
Cross-Linking-MS (XL-MS) Mus musculus
30 Acaa2  
Cross-Linking-MS (XL-MS) Mus musculus
31 Ankrd26  
Cross-Linking-MS (XL-MS) Mus musculus
32 Eed  
Affinity Capture-MS Mus musculus
33 Ndufs2  
Co-fractionation Mus musculus
34 Uqcrc2 67003
Co-fractionation Mus musculus
35 Tia1  
Affinity Capture-MS Mus musculus
36 Fancd2  
Affinity Capture-MS Mus musculus
37 Mdh2 17448
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here