Gene description for Atp5b
Gene name ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit
Gene symbol Atp5b
Other names/aliases -
Species Mus musculus
 Database cross references - Atp5b
ExoCarta ExoCarta_11947
Vesiclepedia VP_11947
Entrez Gene 11947
UniProt P56480  
 Atp5b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Atp5b
Molecular Function
    calcium ion binding GO:0005509 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP hydrolysis activity GO:0016887 IEA
    ATP hydrolysis activity GO:0016887 ISO
    ATP hydrolysis activity GO:0016887 ISO
    lipoprotein particle receptor activity GO:0030228 ISO
    MHC class I protein binding GO:0042288 IEA
    MHC class I protein binding GO:0042288 ISO
    ADP binding GO:0043531 ISO
    angiostatin binding GO:0043532 IEA
    angiostatin binding GO:0043532 ISO
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IBA
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 ISO
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 ISO
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 ISS
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IEA
    proton-transporting ATPase activity, rotational mechanism GO:0046961 ISO
Biological Process
    angiogenesis GO:0001525 IEA
    angiogenesis GO:0001525 ISO
    lipid metabolic process GO:0006629 IMP
    ATP biosynthetic process GO:0006754 ISO
    receptor-mediated endocytosis GO:0006898 ISO
    negative regulation of cell adhesion involved in substrate-bound cell migration GO:0006933 IMP
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 IBA
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 IEA
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 ISO
    positive regulation of blood vessel endothelial cell migration GO:0043536 IEA
    positive regulation of blood vessel endothelial cell migration GO:0043536 ISO
    regulation of intracellular pH GO:0051453 IEA
    regulation of intracellular pH GO:0051453 ISO
    cellular response to interleukin-7 GO:0098761 IDA
    proton transmembrane transport GO:1902600 IEA
    proton transmembrane transport GO:1902600 ISO
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial inner membrane GO:0005743 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    membrane GO:0016020 ISO
    mitochondrial membrane GO:0031966 ISO
    mitochondrial nucleoid GO:0042645 IEA
    mitochondrial nucleoid GO:0042645 ISO
    myelin sheath GO:0043209 HDA
    membrane raft GO:0045121 ISO
    proton-transporting ATP synthase complex GO:0045259 IBA
    proton-transporting ATP synthase complex GO:0045259 ISO
    proton-transporting ATP synthase complex GO:0045259 ISS
    proton-transporting ATP synthase complex, catalytic core F(1) GO:0045261 IBA
    proton-transporting ATP synthase complex, catalytic core F(1) GO:0045261 IEA
    proton-transporting ATP synthase complex, catalytic core F(1) GO:0045261 ISO
 Experiment description of studies that identified Atp5b in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Atp5b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Got2 14719
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
2 Ppif  
Cross-Linking-MS (XL-MS) Mus musculus
3 Idh2 269951
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
4 Cox4i1 12857
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
5 Atp5h 71679
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
6 Atp5j  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
7 Spryd4  
Cross-Linking-MS (XL-MS) Mus musculus
8 Acadm 11364
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
9 Etfb 110826
Cross-Linking-MS (XL-MS) Mus musculus
10 Kat6a  
Cross-Linking-MS (XL-MS) Mus musculus
11 Hadha  
Cross-Linking-MS (XL-MS) Mus musculus
12 Stau1  
Co-fractionation Mus musculus
13 Hadh  
Cross-Linking-MS (XL-MS) Mus musculus
14 Sirt3  
Co-fractionation Mus musculus
15 Ndufs1  
Co-fractionation Mus musculus
16 Inadl  
Affinity Capture-MS Mus musculus
17 Kcnma1  
Affinity Capture-MS Mus musculus
18 D10Jhu81e  
Cross-Linking-MS (XL-MS) Mus musculus
19 Acadl 11363
Cross-Linking-MS (XL-MS) Mus musculus
20 Atp5f1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
21 Kcna3  
Affinity Capture-MS Mus musculus
22 Kctd13  
Affinity Capture-MS Mus musculus
23 Etfa 110842
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
24 Tfe3  
Affinity Capture-MS Mus musculus
25 Uqcrc1  
Cross-Linking-MS (XL-MS) Mus musculus
26 ATP8  
Cross-Linking-MS (XL-MS) Mus musculus
27 Set 56086
Affinity Capture-MS Mus musculus
28 Nnt 18115
Cross-Linking-MS (XL-MS) Mus musculus
29 Decr1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
30 Sf3a1 67465
Cross-Linking-MS (XL-MS) Mus musculus
31 Sdhb 67680
Co-fractionation Mus musculus
32 Atp5b 11947
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
33 Aco2 11429
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
34 Atp5o 28080
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
35 Abcd2  
Cross-Linking-MS (XL-MS) Mus musculus
36 Mettl21e  
Affinity Capture-MS Mus musculus
37 Acot13  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
38 Prdx3 11757
Cross-Linking-MS (XL-MS) Mus musculus
39 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
40 Traf6  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
41 Fh1 14194
Cross-Linking-MS (XL-MS) Mus musculus
42 Sdha 66945
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
43 Vps13b  
Cross-Linking-MS (XL-MS) Mus musculus
44 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
45 Cs 12974
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
46 Usp25 30940
Affinity Capture-MS Mus musculus
47 Aars2  
Cross-Linking-MS (XL-MS) Mus musculus
48 Suclg1  
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
49 Atpif1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
50 Invs  
Affinity Capture-MS Mus musculus
51 Csl  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
52 Sstr3  
Proximity Label-MS Mus musculus
53 Acaa2  
Cross-Linking-MS (XL-MS) Mus musculus
54 Eed  
Affinity Capture-MS Mus musculus
55 Ywhae 22627
Affinity Capture-MS Mus musculus
56 Uqcrc2 67003
Co-fractionation Mus musculus
57 Atg16l1  
Affinity Capture-MS Mus musculus
58 Idh3g  
Co-fractionation Mus musculus
59 Atp5c1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
60 Fancd2  
Affinity Capture-MS Mus musculus
61 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
62 Prdx5 54683
Cross-Linking-MS (XL-MS) Mus musculus
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