Gene description for Fh1
Gene name fumarate hydratase 1
Gene symbol Fh1
Other names/aliases Fh
Fh-1
Species Mus musculus
 Database cross references - Fh1
ExoCarta ExoCarta_14194
Vesiclepedia VP_14194
Entrez Gene 14194
UniProt P97807  
 Fh1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Fh1
Molecular Function
    fumarate hydratase activity GO:0004333 IBA
    fumarate hydratase activity GO:0004333 IMP
    fumarate hydratase activity GO:0004333 ISO
    fumarate hydratase activity GO:0004333 ISS
    histone binding GO:0042393 ISO
Biological Process
    urea cycle GO:0000050 IMP
    urea cycle GO:0000050 IMP
    regulation of arginine metabolic process GO:0000821 IMP
    tricarboxylic acid cycle GO:0006099 IBA
    tricarboxylic acid cycle GO:0006099 IEA
    tricarboxylic acid cycle GO:0006099 IMP
    fumarate metabolic process GO:0006106 IBA
    fumarate metabolic process GO:0006106 IMP
    fumarate metabolic process GO:0006106 ISO
    fumarate metabolic process GO:0006106 ISS
    malate metabolic process GO:0006108 IBA
    malate metabolic process GO:0006108 ISO
    malate metabolic process GO:0006108 ISS
    DNA repair GO:0006281 IEA
    arginine metabolic process GO:0006525 IMP
    DNA damage response GO:0006974 ISO
    DNA damage response GO:0006974 ISS
    homeostasis of number of cells within a tissue GO:0048873 IMP
    positive regulation of cold-induced thermogenesis GO:0120162 IMP
    positive regulation of double-strand break repair via nonhomologous end joining GO:2001034 ISO
    positive regulation of double-strand break repair via nonhomologous end joining GO:2001034 ISS
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    chromosome GO:0005694 IEA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    site of double-strand break GO:0035861 ISO
 Experiment description of studies that identified Fh1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Fh1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Got2 14719
Cross-Linking-MS (XL-MS) Mus musculus
2 Cs 12974
Cross-Linking-MS (XL-MS) Mus musculus
3 Atp5j  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
4 Apoo  
Co-fractionation Mus musculus
5 Coq9  
Co-fractionation Mus musculus
6 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
7 Kcnma1  
Affinity Capture-MS Mus musculus
8 Ogfod1  
Co-fractionation Mus musculus
9 Atp5f1  
Cross-Linking-MS (XL-MS) Mus musculus
10 Atxn1  
Affinity Capture-MS Mus musculus
11 Kctd13  
Affinity Capture-MS Mus musculus
12 HSD17B10 3028
Affinity Capture-MS Homo sapiens
13 Hexb  
Co-fractionation Mus musculus
14 Atp5o 28080
Cross-Linking-MS (XL-MS) Mus musculus
15 Csl  
Cross-Linking-MS (XL-MS) Mus musculus
16 Xpo1 103573
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
17 Atp5j2  
Co-fractionation Mus musculus
18 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
19 Fh1 14194
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
20 Sdha 66945
Co-fractionation Mus musculus
21 Atp5h 71679
Cross-Linking-MS (XL-MS) Mus musculus
22 Tatdn1  
Co-fractionation Mus musculus
23 Atp5b 11947
Cross-Linking-MS (XL-MS) Mus musculus
24 Suclg1  
Cross-Linking-MS (XL-MS) Mus musculus
25 Atic 108147
Co-fractionation Mus musculus
26 Sdhb 67680
Co-fractionation Mus musculus
27 Aco2 11429
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
28 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
29 Uqcrfs1 66694
Co-fractionation Mus musculus
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