Gene description for Prdx5
Gene name peroxiredoxin 5
Gene symbol Prdx5
Other names/aliases AOEB166
AOPP
Pmp20
Prdx6
PrxV
Species Mus musculus
 Database cross references - Prdx5
ExoCarta ExoCarta_54683
Vesiclepedia VP_54683
Entrez Gene 54683
UniProt P99029  
 Prdx5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prdx5
Molecular Function
    RNA polymerase III transcription regulatory region sequence-specific DNA binding GO:0001016 ISO
    peroxidase activity GO:0004601 ISO
    signaling receptor binding GO:0005102 ISO
    signaling receptor binding GO:0005102 ISS
    protein binding GO:0005515 IPI
    thioredoxin peroxidase activity GO:0008379 IBA
    thioredoxin peroxidase activity GO:0008379 IEA
    thioredoxin peroxidase activity GO:0008379 ISO
    antioxidant activity GO:0016209 ISO
    antioxidant activity GO:0016209 ISS
    identical protein binding GO:0042802 ISO
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 ISO
    peroxynitrite reductase activity GO:0072541 ISO
    thioredoxin-dependent peroxiredoxin activity GO:0140824 IDA
Biological Process
    response to oxidative stress GO:0006979 ISO
    response to oxidative stress GO:0006979 ISS
    negative regulation of transcription by RNA polymerase III GO:0016480 ISO
    positive regulation of collagen biosynthetic process GO:0032967 ISO
    cellular response to oxidative stress GO:0034599 IBA
    cellular response to oxidative stress GO:0034599 IEA
    cellular response to reactive oxygen species GO:0034614 ISO
    hydrogen peroxide catabolic process GO:0042744 IBA
    hydrogen peroxide catabolic process GO:0042744 ISO
    hydrogen peroxide catabolic process GO:0042744 ISS
    negative regulation of apoptotic process GO:0043066 ISO
    cell redox homeostasis GO:0045454 IBA
    negative regulation of oxidoreductase activity GO:0051354 ISO
    regulation of apoptosis involved in tissue homeostasis GO:0060785 ISO
    NADPH oxidation GO:0070995 ISO
    cellular oxidant detoxification GO:0098869 IEA
    reactive nitrogen species metabolic process GO:2001057 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    peroxisome GO:0005777 IBA
    peroxisome GO:0005777 ISO
    peroxisomal matrix GO:0005782 ISO
    peroxisomal matrix GO:0005782 ISS
    cytosol GO:0005829 ISO
    cytoplasmic vesicle GO:0031410 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Prdx5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prdx5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Csl  
Cross-Linking-MS (XL-MS) Mus musculus
2 Hsbp1  
Co-fractionation Mus musculus
3 Ncoa3  
Two-hybrid Mus musculus
4 Rpl32 19951
Co-fractionation Mus musculus
5 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
6 Kctd13  
Affinity Capture-MS Mus musculus
7 Sufu  
Two-hybrid Mus musculus
8 MAPT  
Reconstituted Complex Homo sapiens
9 Atp5b 11947
Cross-Linking-MS (XL-MS) Mus musculus
10 HSD17B10 3028
Affinity Capture-MS Homo sapiens
11 Hspd1 15510
Cross-Linking-MS (XL-MS) Mus musculus
12 Fancd2  
Affinity Capture-MS Mus musculus
13 Slc4a2  
Co-fractionation Mus musculus
14 Prdx5 54683
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
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