Gene description for Ogdh
Gene name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Gene symbol Ogdh
Other names/aliases 2210403E04Rik
2210412K19Rik
AA409584
d1401
mKIAA4192
Species Mus musculus
 Database cross references - Ogdh
ExoCarta ExoCarta_18293
Vesiclepedia VP_18293
Entrez Gene 18293
UniProt Q60597  
 Ogdh identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Melanoma cells 34957415    
Melanoma cells 34957415    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ogdh
Molecular Function
    oxoglutarate dehydrogenase (succinyl-transferring) activity GO:0004591 IBA
    oxoglutarate dehydrogenase (succinyl-transferring) activity GO:0004591 ISO
    oxoglutarate dehydrogenase (succinyl-transferring) activity GO:0004591 ISS
    thiamine pyrophosphate binding GO:0030976 ISO
    thiamine pyrophosphate binding GO:0030976 ISS
    heat shock protein binding GO:0031072 ISO
    oxoglutarate dehydrogenase (NAD+) activity GO:0034602 IDA
    metal ion binding GO:0046872 IEA
    protein-folding chaperone binding GO:0051087 ISO
Biological Process
    generation of precursor metabolites and energy GO:0006091 ISS
    glycolytic process GO:0006096 IEA
    tricarboxylic acid cycle GO:0006099 IBA
    tricarboxylic acid cycle GO:0006099 ISO
    2-oxoglutarate metabolic process GO:0006103 ISO
    2-oxoglutarate metabolic process GO:0006103 ISS
    succinyl-CoA metabolic process GO:0006104 ISO
    succinyl-CoA metabolic process GO:0006104 ISS
    NADH metabolic process GO:0006734 ISO
    cerebellar cortex development GO:0021695 IEP
    striatum development GO:0021756 IEP
    hippocampus development GO:0021766 IEP
    thalamus development GO:0021794 IEP
    pyramidal neuron development GO:0021860 IEP
    tangential migration from the subventricular zone to the olfactory bulb GO:0022028 IEP
    olfactory bulb mitral cell layer development GO:0061034 IEP
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrial membrane GO:0031966 ISS
    oxoglutarate dehydrogenase complex GO:0045252 IBA
    oxoglutarate dehydrogenase complex GO:0045252 IMP
    oxoglutarate dehydrogenase complex GO:0045252 ISO
    oxoglutarate dehydrogenase complex GO:0045252 ISS
 Experiment description of studies that identified Ogdh in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1117
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1118
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ogdh
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sdhb 67680
Co-fractionation Mus musculus
2 Ogdh 18293
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
3 Atxn1  
Affinity Capture-MS Mus musculus
4 Ogdhl  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Sdha 66945
Co-fractionation Mus musculus
7 Hadha  
Cross-Linking-MS (XL-MS) Mus musculus
8 Dld 13382
Cross-Linking-MS (XL-MS) Mus musculus
9 HSD17B10 3028
Affinity Capture-MS Homo sapiens
10 Unk  
Affinity Capture-RNA Mus musculus
11 Hspb2 69253
Affinity Capture-MS Mus musculus
12 Fancd2  
Affinity Capture-MS Mus musculus
13 Dlst  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
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