Gene description for Pdhb
Gene name pyruvate dehydrogenase (lipoamide) beta
Gene symbol Pdhb
Other names/aliases 2610103L06Rik
AL024199
C81408
Species Mus musculus
 Database cross references - Pdhb
ExoCarta ExoCarta_68263
Vesiclepedia VP_68263
Entrez Gene 68263
UniProt Q9D051  
 Pdhb identified in sEVs derived from the following tissue/cell type
Melanoma cells 34957415    
Melanoma cells 34957415    
 Gene ontology annotations for Pdhb
Molecular Function
    pyruvate dehydrogenase (acetyl-transferring) activity GO:0004739 IBA
    pyruvate dehydrogenase (acetyl-transferring) activity GO:0004739 ISO
    pyruvate dehydrogenase (acetyl-transferring) activity GO:0004739 ISO
    pyruvate dehydrogenase (acetyl-transferring) activity GO:0004739 ISS
    pyruvate dehydrogenase (NAD+) activity GO:0034604 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    glucose metabolic process GO:0006006 IEA
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 IBA
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 ISO
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 ISO
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 ISS
    tricarboxylic acid cycle GO:0006099 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 IEA
    pyruvate dehydrogenase complex GO:0045254 IBA
    pyruvate dehydrogenase complex GO:0045254 ISO
    pyruvate dehydrogenase complex GO:0045254 ISS
    acetyltransferase complex GO:1902493 ISO
    catalytic complex GO:1902494 ISO
 Experiment description of studies that identified Pdhb in sEVs
1
Experiment ID 1117
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1118
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pdhb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tia1  
Affinity Capture-MS Mus musculus
2 Chchd3  
Co-fractionation Mus musculus
3 Pdha2  
Cross-Linking-MS (XL-MS) Mus musculus
4 Sdha 66945
Co-fractionation Mus musculus
5 Atxn1  
Affinity Capture-MS Mus musculus
6 Pdha1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
7 Atp5a1 11946
Co-fractionation Mus musculus
8 Kctd13  
Affinity Capture-MS Mus musculus
9 Uqcrc2 67003
Co-fractionation Mus musculus
10 Ndufab1  
Co-fractionation Mus musculus
11 Mapt  
Affinity Capture-MS Mus musculus
12 Atg16l1  
Affinity Capture-MS Mus musculus
13 HSD17B10 3028
Affinity Capture-MS Homo sapiens
14 Idh3g  
Co-fractionation Mus musculus
15 Sucla2  
Co-fractionation Mus musculus
16 Nanog  
Affinity Capture-MS Mus musculus
17 Fancd2  
Affinity Capture-MS Mus musculus
18 Ywhaz 22631
Affinity Capture-MS Mus musculus
19 Suclg1  
Co-fractionation Mus musculus
20 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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