Gene description for Slc25a5
Gene name solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 5
Gene symbol Slc25a5
Other names/aliases Ant2
Species Mus musculus
 Database cross references - Slc25a5
ExoCarta ExoCarta_11740
Vesiclepedia VP_11740
Entrez Gene 11740
UniProt P51881  
 Slc25a5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Mov neuroglial cells 15210972    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slc25a5
Molecular Function
    adenine nucleotide transmembrane transporter activity GO:0000295 ISO
    ATP:ADP antiporter activity GO:0005471 IDA
    ATP:ADP antiporter activity GO:0005471 IMP
    protein binding GO:0005515 IPI
    proton transmembrane transporter activity GO:0015078 IDA
    oxidative phosphorylation uncoupler activity GO:0017077 IDA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
Biological Process
    chromosome segregation GO:0007059 IEA
    positive regulation of cell population proliferation GO:0008284 ISO
    positive regulation of cell population proliferation GO:0008284 ISS
    B cell differentiation GO:0030183 IMP
    erythrocyte differentiation GO:0030218 IMP
    regulation of mitochondrial membrane permeability GO:0046902 IMP
    adenine nucleotide transport GO:0051503 ISO
    mitochondrial ADP transmembrane transport GO:0140021 IDA
    mitochondrial ADP transmembrane transport GO:0140021 IMP
    negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901029 IBA
    negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901029 ISO
    negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901029 ISS
    positive regulation of mitophagy GO:1901526 IDA
    proton transmembrane transport GO:1902600 IEA
    mitochondrial ATP transmembrane transport GO:1990544 IDA
    mitochondrial ATP transmembrane transport GO:1990544 IMP
    cellular response to leukemia inhibitory factor GO:1990830 IEP
    adaptive thermogenesis GO:1990845 IMP
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial permeability transition pore complex GO:0005757 IMP
    membrane GO:0016020 ISO
    mitochondrial nucleoid GO:0042645 IEA
    mitochondrial nucleoid GO:0042645 ISO
    myelin sheath GO:0043209 HDA
    membrane raft GO:0045121 ISO
    MMXD complex GO:0071817 ISO
    MMXD complex GO:0071817 ISS
 Experiment description of studies that identified Slc25a5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slc25a5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nphs2  
Affinity Capture-MS Mus musculus
2 Cbx1  
Affinity Capture-MS Mus musculus
3 Ubc  
Reconstituted Complex Mus musculus
4 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
5 Hspb2 69253
Affinity Capture-MS Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Psma3 19167
Co-fractionation Mus musculus
8 Atxn1  
Proximity Label-MS Mus musculus
9 Kctd13  
Affinity Capture-MS Mus musculus
10 Thrb  
Affinity Capture-MS Mus musculus
11 Cox6c  
Co-fractionation Mus musculus
12 Tfe3  
Affinity Capture-MS Mus musculus
13 Atp5l  
Co-fractionation Mus musculus
14 Ywhae 22627
Affinity Capture-MS Mus musculus
15 Mettl21e  
Affinity Capture-MS Mus musculus
16 Tmem173  
Proximity Label-MS Mus musculus
17 Slc25a5 11740
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
18 Cbx3 12417
Affinity Capture-MS Mus musculus
19 Sdhb 67680
Co-fractionation Mus musculus
20 Mapt  
Affinity Capture-MS Mus musculus
21 Stau1  
Co-fractionation Mus musculus
22 Atp5c1  
Co-fractionation Mus musculus
23 Fancd2  
Affinity Capture-MS Mus musculus
24 Mdh2 17448
Co-fractionation Mus musculus
25 Lipc  
Affinity Capture-MS Mus musculus
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