Gene description for Hk1
Gene name hexokinase 1
Gene symbol Hk1
Other names/aliases BB404130
Hk-1
Hk1-s
dea
mHk1-s
Species Mus musculus
 Database cross references - Hk1
ExoCarta ExoCarta_15275
Vesiclepedia VP_15275
Entrez Gene 15275
UniProt P17710  
 Hk1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hk1
Molecular Function
    glucokinase activity GO:0004340 IBA
    glucokinase activity GO:0004340 IDA
    glucokinase activity GO:0004340 ISO
    glucokinase activity GO:0004340 ISS
    hexokinase activity GO:0004396 IDA
    hexokinase activity GO:0004396 IMP
    hexokinase activity GO:0004396 ISO
    hexokinase activity GO:0004396 TAS
    protein kinase activity GO:0004672 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    D-glucose binding GO:0005536 IEA
    D-glucose binding GO:0005536 ISO
    fructokinase activity GO:0008865 IBA
    fructokinase activity GO:0008865 ISO
    fructokinase activity GO:0008865 ISS
    mannokinase activity GO:0019158 IBA
    mannokinase activity GO:0019158 ISO
    mannokinase activity GO:0019158 ISS
    identical protein binding GO:0042802 ISO
    peptidoglycan binding GO:0042834 IDA
    peptidoglycan binding GO:0042834 ISO
    protein-containing complex binding GO:0044877 ISO
    glucosamine kinase activity GO:0047931 IEA
Biological Process
    intracellular glucose homeostasis GO:0001678 IBA
    intracellular glucose homeostasis GO:0001678 IEA
    positive regulation of cytokine production involved in immune response GO:0002720 IDA
    response to ischemia GO:0002931 ISO
    fructose 6-phosphate metabolic process GO:0006002 ISO
    fructose 6-phosphate metabolic process GO:0006002 ISS
    glucose metabolic process GO:0006006 IBA
    mannose metabolic process GO:0006013 ISO
    mannose metabolic process GO:0006013 ISS
    glycolytic process GO:0006096 IBA
    glycolytic process GO:0006096 ISO
    glycolytic process GO:0006096 TAS
    inflammatory response GO:0006954 IEA
    positive regulation of interleukin-1 beta production GO:0032731 IDA
    negative regulation of apoptotic process GO:0043066 ISO
    innate immune response GO:0045087 IEA
    carbohydrate phosphorylation GO:0046835 IMP
    carbohydrate phosphorylation GO:0046835 ISO
    glucose 6-phosphate metabolic process GO:0051156 IBA
    glucose 6-phosphate metabolic process GO:0051156 IEA
    glucose 6-phosphate metabolic process GO:0051156 ISO
    glucose 6-phosphate metabolic process GO:0051156 ISS
    canonical glycolysis GO:0061621 IDA
    GDP-mannose biosynthetic process from mannose GO:0061728 IDA
    establishment of protein localization to mitochondrion GO:0072655 IEA
    establishment of protein localization to mitochondrion GO:0072655 ISO
    maintenance of protein location in mitochondrion GO:0072656 IEA
    maintenance of protein location in mitochondrion GO:0072656 ISO
    GDP-mannose biosynthetic process from glucose GO:0141199 IDA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrial outer membrane GO:0005741 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    caveola GO:0005901 ISO
    protein-containing complex GO:0032991 ISO
    membrane raft GO:0045121 IDA
 Experiment description of studies that identified Hk1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hk1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Twf2  
Co-fractionation Mus musculus
2 Uba5  
Co-fractionation Mus musculus
3 HTT 3064
Affinity Capture-MS Homo sapiens
4 SNCA 6622
Affinity Capture-MS Homo sapiens
5 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
6 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
8 Vdac2 22334
Co-fractionation Mus musculus
9 MAPT  
Reconstituted Complex Homo sapiens
10 Mapt  
Affinity Capture-MS Mus musculus
11 Stau1  
Co-fractionation Mus musculus
12 Sgta 52551
Co-fractionation Mus musculus
13 Iqcb1  
Affinity Capture-MS Mus musculus
14 Hk1 15275
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
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