Gene description for Idh3a
Gene name isocitrate dehydrogenase 3 (NAD+) alpha
Gene symbol Idh3a
Other names/aliases 1110003P10Rik
1500012E04Rik
AA407078
AI316514
Species Mus musculus
 Database cross references - Idh3a
ExoCarta ExoCarta_67834
Vesiclepedia VP_67834
Entrez Gene 67834
UniProt Q9D6R2  
 Idh3a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Idh3a
Molecular Function
    magnesium ion binding GO:0000287 ISO
    magnesium ion binding GO:0000287 ISS
    isocitrate dehydrogenase (NAD+) activity GO:0004449 IBA
    isocitrate dehydrogenase (NAD+) activity GO:0004449 ISO
    isocitrate dehydrogenase (NAD+) activity GO:0004449 ISS
    NAD binding GO:0051287 IEA
Biological Process
    tricarboxylic acid cycle GO:0006099 IBA
    tricarboxylic acid cycle GO:0006099 ISO
    isocitrate metabolic process GO:0006102 IBA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 NAS
    myelin sheath GO:0043209 HDA
    isocitrate dehydrogenase complex (NAD+) GO:0045242 ISO
 Experiment description of studies that identified Idh3a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Idh3a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Acn9  
Co-fractionation Mus musculus
2 Got2 14719
Cross-Linking-MS (XL-MS) Mus musculus
3 Idh2 269951
Cross-Linking-MS (XL-MS) Mus musculus
4 Atp5j  
Cross-Linking-MS (XL-MS) Mus musculus
5 Slc25a4 11739
Co-fractionation Mus musculus
6 Cisd1  
Co-fractionation Mus musculus
7 Kcnma1  
Affinity Capture-MS Mus musculus
8 Ndufa12  
Co-fractionation Mus musculus
9 Atxn1  
Affinity Capture-MS Mus musculus
10 Kctd13  
Affinity Capture-MS Mus musculus
11 HSD17B10 3028
Affinity Capture-MS Homo sapiens
12 Sucla2  
Co-fractionation Mus musculus
13 Immt  
Cross-Linking-MS (XL-MS) Mus musculus
14 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
15 Ndufa5  
Co-fractionation Mus musculus
16 Idh3a 67834
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
17 Mapt  
Affinity Capture-MS Mus musculus
18 Suclg1  
Co-fractionation Mus musculus
19 Idh3b 170718
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
20 Chchd3  
Co-fractionation Mus musculus
21 Uqcrc1  
Cross-Linking-MS (XL-MS) Mus musculus
22 Uqcrc2 67003
Co-fractionation Mus musculus
23 Tia1  
Affinity Capture-MS Mus musculus
24 Idh3g  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
25 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
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