Gene description for Cand1
Gene name cullin associated and neddylation disassociated 1
Gene symbol Cand1
Other names/aliases 2310038O07Rik
6330512O03Rik
AI195005
AI846556
D10Ertd516e
Tp120a
mKIAA0829
Species Mus musculus
 Database cross references - Cand1
ExoCarta ExoCarta_71902
Vesiclepedia VP_71902
Entrez Gene 71902
UniProt Q6ZQ38  
 Cand1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cand1
Molecular Function
    protein binding GO:0005515 IPI
    TBP-class protein binding GO:0017025 IEA
    TBP-class protein binding GO:0017025 ISO
Biological Process
    SCF complex assembly GO:0010265 IBA
    SCF complex assembly GO:0010265 ISO
    SCF complex assembly GO:0010265 ISS
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 ISO
    protein ubiquitination GO:0016567 ISS
    cell differentiation GO:0030154 ISO
    cell differentiation GO:0030154 ISS
    negative regulation of catalytic activity GO:0043086 ISO
    negative regulation of catalytic activity GO:0043086 ISS
    positive regulation of DNA-templated transcription GO:0045893 ISO
    positive regulation of DNA-templated transcription GO:0045893 ISS
    positive regulation of RNA polymerase II transcription preinitiation complex assembly GO:0045899 IEA
    positive regulation of RNA polymerase II transcription preinitiation complex assembly GO:0045899 ISO
Subcellular Localization
    ubiquitin ligase complex GO:0000151 ISO
    ubiquitin ligase complex GO:0000151 ISS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cullin-RING ubiquitin ligase complex GO:0031461 ISO
    cullin-RING ubiquitin ligase complex GO:0031461 ISS
 Experiment description of studies that identified Cand1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cand1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Stub1 56424
Co-fractionation Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Syngap1  
Affinity Capture-MS Mus musculus
4 Atxn1  
Affinity Capture-MS Mus musculus
5 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Cul1 26965
Affinity Capture-Western Mus musculus
8 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
9 Mapt  
Affinity Capture-MS Mus musculus
10 MAPT  
Reconstituted Complex Homo sapiens
11 Chaf1a  
Affinity Capture-MS Mus musculus
12 Unk  
Affinity Capture-RNA Mus musculus
13 Ube2v1 66589
Co-fractionation Mus musculus
14 Fancd2  
Affinity Capture-MS Mus musculus
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