Gene description for Cul1
Gene name cullin 1
Gene symbol Cul1
Other names/aliases -
Species Mus musculus
 Database cross references - Cul1
ExoCarta ExoCarta_26965
Vesiclepedia VP_26965
Entrez Gene 26965
UniProt Q9WTX6  
 Cul1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cul1
Molecular Function
    protein binding GO:0005515 IPI
    protein-macromolecule adaptor activity GO:0030674 IBA
    ubiquitin protein ligase binding GO:0031625 IBA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    ubiquitin protein ligase activity GO:0061630 IC
    ubiquitin ligase complex scaffold activity GO:0160072 IEA
    ubiquitin ligase complex scaffold activity GO:0160072 ISO
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IC
    protein monoubiquitination GO:0006513 IGI
    apoptotic process GO:0006915 IMP
    cell population proliferation GO:0008283 IMP
    animal organ morphogenesis GO:0009887 IMP
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 ISO
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 IBA
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 IDA
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 ISO
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IEA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    protein K48-linked ubiquitination GO:0070936 IDA
    protein K48-linked ubiquitination GO:0070936 ISO
Subcellular Localization
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    SCF ubiquitin ligase complex GO:0019005 IBA
    SCF ubiquitin ligase complex GO:0019005 IDA
    SCF ubiquitin ligase complex GO:0019005 ISO
    SCF ubiquitin ligase complex GO:0019005 ISS
    cullin-RING ubiquitin ligase complex GO:0031461 ISO
    Parkin-FBXW7-Cul1 ubiquitin ligase complex GO:1990452 IEA
    Parkin-FBXW7-Cul1 ubiquitin ligase complex GO:1990452 ISO
 Experiment description of studies that identified Cul1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cul1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RALBP1 10928
Affinity Capture-MS Homo sapiens
2 PSMD5 5711
Affinity Capture-MS Homo sapiens
3 Btrc  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
4 RECQL5  
Affinity Capture-MS Homo sapiens
5 NUP54 53371
Affinity Capture-MS Homo sapiens
6 Wwtr1  
Affinity Capture-Western Mus musculus
7 IARS2 55699
Affinity Capture-MS Homo sapiens
8 GPS1 2873
Affinity Capture-MS Homo sapiens
9 SF1 7536
Affinity Capture-MS Homo sapiens
10 FBXO7 25793
Affinity Capture-MS Homo sapiens
11 USP11 8237
Affinity Capture-MS Homo sapiens
12 Fbxw8  
Affinity Capture-Western Mus musculus
13 MKNK1  
Affinity Capture-MS Homo sapiens
14 Dusp1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
15 Skp1a 21402
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
16 DR1  
Affinity Capture-MS Homo sapiens
17 Fbxo15  
Affinity Capture-Western Mus musculus
18 EME1  
Affinity Capture-MS Homo sapiens
19 SKP2  
Affinity Capture-MS Homo sapiens
20 LTN1 26046
Affinity Capture-MS Homo sapiens
21 TTC3 7267
Affinity Capture-MS Homo sapiens
22 RBX1 9978
Affinity Capture-MS Homo sapiens
23 Rbx1  
Affinity Capture-Western Mus musculus
24 ZC3HC1  
Affinity Capture-Western Homo sapiens
25 CAND1 55832
Affinity Capture-MS Homo sapiens
26 MRPL52  
Affinity Capture-MS Homo sapiens
27 Fbxo44 230903
Co-fractionation Mus musculus
28 SYNE2 23224
Affinity Capture-MS Homo sapiens
29 UTP11L  
Affinity Capture-MS Homo sapiens
30 Mpeg1  
Affinity Capture-Western Mus musculus
31 Fbxw4 30838
Affinity Capture-Western Mus musculus
32 Irs1  
Affinity Capture-Western Mus musculus
33 Fbxo2  
Affinity Capture-Western Mus musculus
34 Myog  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
35 CPEB2  
Affinity Capture-MS Homo sapiens
36 ARMC6 93436
Affinity Capture-MS Homo sapiens
37 Fbxl3  
PCA Mus musculus
38 Pkd2  
Affinity Capture-Western Mus musculus
39 ZCCHC17  
Affinity Capture-MS Homo sapiens
40 Chek1  
Affinity Capture-Western Mus musculus
41 RPUSD4 84881
Affinity Capture-MS Homo sapiens
42 Cops2 12848
Affinity Capture-Western Mus musculus
43 CDCA3 83461
Affinity Capture-MS Homo sapiens
44 DPF2  
Affinity Capture-MS Homo sapiens
45 Fbxo32  
Affinity Capture-Western Mus musculus
46 Fbxw2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
47 GABPB1  
Affinity Capture-MS Homo sapiens
48 Cand2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
49 FOXM1  
Affinity Capture-MS Homo sapiens
50 YWHAQ 10971
Affinity Capture-MS Homo sapiens
51 Mcl1  
Affinity Capture-Western Mus musculus
52 Cand1 71902
Affinity Capture-Western Mus musculus
53 SLC39A10 57181
Affinity Capture-MS Homo sapiens
54 Eed  
Affinity Capture-MS Mus musculus
55 Fbxl20  
Affinity Capture-Western Mus musculus
56 Fbxw7  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
57 COPS3 8533
Affinity Capture-MS Homo sapiens
58 Fbxo40  
Affinity Capture-Western Mus musculus
59 ADGRE5 976
Affinity Capture-MS Homo sapiens
60 IL18 3606
Affinity Capture-MS Homo sapiens
61 GOLGA8R  
Affinity Capture-MS Homo sapiens
62 Skp2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
63 Fbxl21  
PCA Mus musculus
View the network image/svg+xml
 Pathways in which Cul1 is involved
PathwayEvidenceSource
Activation of NF-kappaB in B cells IEA Reactome
Adaptive Immune System IEA Reactome
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome
APC/C-mediated degradation of cell cycle proteins IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to chemical stress IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Class I MHC mediated antigen processing & presentation IEA Reactome
CLEC7A (Dectin-1) signaling IEA Reactome
Cyclin A:Cdk2-associated events at S phase entry IEA Reactome
Cyclin D associated events in G1 IEA Reactome
Cyclin E associated events during G1/S transition IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Dectin-1 mediated noncanonical NF-kB signaling IEA Reactome
Degradation of beta-catenin by the destruction complex IEA Reactome
Degradation of GLI1 by the proteasome IEA Reactome
DNA Replication IEA Reactome
Downstream signaling events of B Cell Receptor (BCR) IEA Reactome
Downstream TCR signaling IEA Reactome
FBXL7 down-regulates AURKA during mitotic entry and in early mitosis IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated NF-kB activation IEA Reactome
G1 Phase IEA Reactome
G1/S Transition IEA Reactome
G2/M Transition IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
GLI3 is processed to GLI3R by the proteasome IEA Reactome
GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 IEA Reactome
Hedgehog 'off' state IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Interleukin-1 family signaling IEA Reactome
Interleukin-1 signaling IEA Reactome
Interleukin-17 signaling IEA Reactome
Iron uptake and transport IEA Reactome
KEAP1-NFE2L2 pathway IEA Reactome
MAP kinase activation IEA Reactome
MAP3K8 (TPL2)-dependent MAPK1/3 activation IEA Reactome
Metabolism of proteins IEA Reactome
Mitotic G1 phase and G1/S transition IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
MyD88 cascade initiated on plasma membrane IEA Reactome
MyD88 dependent cascade initiated on endosome IEA Reactome
MyD88-independent TLR4 cascade IEA Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane IEA Reactome
Neddylation IEA Reactome
NIK-->noncanonical NF-kB signaling IEA Reactome
Nuclear events mediated by NFE2L2 IEA Reactome
Orc1 removal from chromatin IEA Reactome
Post-translational protein modification IEA Reactome
Prolactin receptor signaling IEA Reactome
Regulation of APC/C activators between G1/S and early anaphase IEA Reactome
Regulation of BACH1 activity IEA Reactome
Regulation of mitotic cell cycle IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Regulation of RUNX2 expression and activity IEA Reactome
RNA Polymerase II Transcription IEA Reactome
S Phase IEA Reactome
SCF(Skp2)-mediated degradation of p27/p21 IEA Reactome
SCF-beta-TrCP mediated degradation of Emi1 IEA Reactome
Signal Transduction IEA Reactome
Signaling by Hedgehog IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by the B Cell Receptor (BCR) IEA Reactome
Signaling by WNT IEA Reactome
Switching of origins to a post-replicative state IEA Reactome
Synthesis of DNA IEA Reactome
TCR signaling IEA Reactome
TNFR2 non-canonical NF-kB pathway IEA Reactome
Toll Like Receptor 10 (TLR10) Cascade IEA Reactome
Toll Like Receptor 2 (TLR2) Cascade IEA Reactome
Toll Like Receptor 3 (TLR3) Cascade IEA Reactome
Toll Like Receptor 4 (TLR4) Cascade IEA Reactome
Toll Like Receptor 5 (TLR5) Cascade IEA Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade IEA Reactome
Toll Like Receptor 9 (TLR9) Cascade IEA Reactome
Toll Like Receptor TLR1:TLR2 Cascade IEA Reactome
Toll Like Receptor TLR6:TLR2 Cascade IEA Reactome
Toll-like Receptor Cascades IEA Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation IEA Reactome
Transcriptional regulation by RUNX2 IEA Reactome
Transport of small molecules IEA Reactome
TRIF (TICAM1)-mediated TLR4 signaling IEA Reactome





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