Gene description for Cops2
Gene name COP9 (constitutive photomorphogenic) homolog, subunit 2 (Arabidopsis thaliana)
Gene symbol Cops2
Other names/aliases AI315723
C85265
Csn2
Sgn2
TRIP-15
Trip15
Species Mus musculus
 Database cross references - Cops2
ExoCarta ExoCarta_12848
Vesiclepedia VP_12848
Entrez Gene 12848
UniProt P61202  
 Cops2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cops2
Molecular Function
    transcription corepressor activity GO:0003714 IDA
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    protein deneddylation GO:0000338 IBA
    protein deneddylation GO:0000338 IEA
    protein deneddylation GO:0000338 ISO
    inner cell mass cell proliferation GO:0001833 IMP
    trophectodermal cell proliferation GO:0001834 IMP
    protein phosphorylation GO:0006468 ISO
    neuron differentiation GO:0030182 IEA
    neuron differentiation GO:0030182 ISO
    skeletal muscle cell differentiation GO:0035914 IMP
    negative regulation of DNA-templated transcription GO:0045892 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    COP9 signalosome GO:0008180 IBA
    COP9 signalosome GO:0008180 IDA
    COP9 signalosome GO:0008180 ISO
    COP9 signalosome GO:0008180 ISS
 Experiment description of studies that identified Cops2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cops2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nap1l1 53605
Two-hybrid Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
3 Btrc  
Affinity Capture-Western Mus musculus
4 CUL1 8454
Affinity Capture-MS Homo sapiens
5 GPS1 2873
Affinity Capture-Western Homo sapiens
6 Cul1 26965
Affinity Capture-Western Mus musculus
7 Ctnnb1 12387
Affinity Capture-Western Mus musculus
8 Axin1  
Affinity Capture-Western Mus musculus
9 Lrp6  
Affinity Capture-Western Mus musculus
10 Cops5 26754
Affinity Capture-Western Mus musculus
11 Atxn1  
Affinity Capture-MS Mus musculus
12 Thrb  
Co-localization Mus musculus
Co-localization Mus musculus
13 Rbx1  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
14 Csnk1a1 93687
Biochemical Activity Mus musculus
15 Cops7a 26894
Affinity Capture-Western Mus musculus
16 Psmd5 66998
Co-fractionation Mus musculus
17 Cops6 26893
Affinity Capture-Western Mus musculus
18 Setdb1  
Co-localization Mus musculus
Co-localization Mus musculus
Affinity Capture-Western Mus musculus
19 Hoxd12  
Two-hybrid Mus musculus
20 Cops3  
Affinity Capture-Western Mus musculus
21 Cops8 108679
Affinity Capture-Western Mus musculus
22 Gsk3b  
Affinity Capture-Western Mus musculus
23 Cops4 26891
Affinity Capture-Western Mus musculus
24 Gps1 209318
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
25 Apc  
Affinity Capture-Western Mus musculus
View the network image/svg+xml



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