Gene description for Skp1a
Gene name S-phase kinase-associated protein 1A
Gene symbol Skp1a
Other names/aliases 15kDa
2610043E24Rik
2610206H23Rik
EMC19
OCP-II
OCP2
SKP1
Tceb1l
p19A
p19Skp1
Species Mus musculus
 Database cross references - Skp1a
ExoCarta ExoCarta_21402
Vesiclepedia VP_21402
Entrez Gene 21402
UniProt Q9WTX5  
 Skp1a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Skp1a
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IC
    protein binding GO:0005515 IPI
    beta-catenin binding GO:0008013 IEA
    beta-catenin binding GO:0008013 ISO
    protein domain specific binding GO:0019904 ISO
    cullin family protein binding GO:0097602 IBA
    cullin family protein binding GO:0097602 IEA
    cullin family protein binding GO:0097602 ISO
    molecular function activator activity GO:0140677 IEA
    molecular function activator activity GO:0140677 ISO
    ubiquitin ligase complex scaffold activity GO:0160072 IEA
    ubiquitin ligase complex scaffold activity GO:0160072 ISO
    F-box domain binding GO:1990444 IEA
    F-box domain binding GO:1990444 ISO
    ubiquitin-like ligase-substrate adaptor activity GO:1990756 IEA
    ubiquitin-like ligase-substrate adaptor activity GO:1990756 ISO
Biological Process
    protein polyubiquitination GO:0000209 ISO
    chromatin remodeling GO:0006338 IEA
    chromatin remodeling GO:0006338 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 IC
    protein monoubiquitination GO:0006513 IGI
    protein ubiquitination GO:0016567 ISO
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 IBA
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 IDA
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    maintenance of protein location in nucleus GO:0051457 IEA
    maintenance of protein location in nucleus GO:0051457 ISO
    protein K48-linked ubiquitination GO:0070936 IDA
    protein K48-linked ubiquitination GO:0070936 ISO
    positive regulation of ubiquitin protein ligase activity GO:1904668 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    gap junction GO:0005921 ISO
    SCF ubiquitin ligase complex GO:0019005 IDA
    SCF ubiquitin ligase complex GO:0019005 ISO
    SCF ubiquitin ligase complex GO:0019005 ISS
    Cul7-RING ubiquitin ligase complex GO:0031467 IDA
    Cul7-RING ubiquitin ligase complex GO:0031467 ISO
    PcG protein complex GO:0031519 IEA
    PcG protein complex GO:0031519 ISO
 Experiment description of studies that identified Skp1a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Skp1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cul2 71745
Co-fractionation Mus musculus
2 PSMA6 5687
Affinity Capture-MS Homo sapiens
3 ABCF3 55324
Affinity Capture-MS Homo sapiens
4 Cul1 26965
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
5 FBXO7 25793
Affinity Capture-MS Homo sapiens
6 Tcp1 21454
Affinity Capture-Western Mus musculus
7 KIAA2013 90231
Affinity Capture-MS Homo sapiens
8 TANC1 85461
Affinity Capture-MS Homo sapiens
9 Cul3  
Co-fractionation Mus musculus
10 Rbx1  
Co-fractionation Mus musculus
11 Ppp1ca 19045
Affinity Capture-Western Mus musculus
12 Fbxo2  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
13 Tmem173  
Proximity Label-MS Mus musculus
14 Myog  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
15 Fbxo32  
Affinity Capture-Western Mus musculus
16 FBXO46  
Affinity Capture-MS Homo sapiens
17 KDM2A  
Affinity Capture-MS Homo sapiens
18 PSMB6 5694
Affinity Capture-MS Homo sapiens
19 Cenpe  
Co-fractionation Mus musculus
20 CUL1 8454
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 BTRC 8945
Affinity Capture-MS Homo sapiens
22 FBXW5  
Affinity Capture-MS Homo sapiens
23 GAR1 54433
Affinity Capture-MS Homo sapiens
24 Cdkn1b  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
25 Faf1  
Co-fractionation Mus musculus
26 Fbxo40  
Affinity Capture-Western Mus musculus
27 Kdm2b  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
28 Fbxw4 30838
Affinity Capture-Western Mus musculus
29 Fbxo15  
Affinity Capture-Western Mus musculus
30 ACTL8  
Affinity Capture-MS Homo sapiens
31 Ppp1cc 19047
Affinity Capture-Western Mus musculus
32 SPRYD3 84926
Affinity Capture-MS Homo sapiens
33 Mcl1  
Affinity Capture-Western Mus musculus
34 Fbxl20  
Affinity Capture-Western Mus musculus
35 Cops8 108679
Co-fractionation Mus musculus
36 Nedd8  
Co-fractionation Mus musculus
37 Ccdc6  
Co-fractionation Mus musculus
38 EPHB4 2050
Affinity Capture-MS Homo sapiens
39 Tcf3  
Affinity Capture-MS Mus musculus
40 FBXO45 200933
Affinity Capture-MS Homo sapiens
41 Mycbp2 105689
Affinity Capture-MS Mus musculus
42 PSMA3 5684
Affinity Capture-MS Homo sapiens
43 Hspa8 15481
Co-fractionation Mus musculus
44 FANCD2  
Affinity Capture-MS Homo sapiens
45 MYCBP2 23077
Affinity Capture-MS Homo sapiens
46 Fbxl15  
Co-fractionation Mus musculus
47 XPO1 7514
Affinity Capture-MS Homo sapiens
48 Sugt1 67955
Co-fractionation Mus musculus
49 SKP2  
Affinity Capture-MS Homo sapiens
50 Fbxo4  
Affinity Capture-Western Mus musculus
51 Fbxl16  
Affinity Capture-Western Mus musculus
52 Fbxo44 230903
Co-fractionation Mus musculus
53 CKS1B  
Affinity Capture-MS Homo sapiens
54 Mtss1  
Co-fractionation Mus musculus
55 MTUS1  
Affinity Capture-MS Homo sapiens
56 C16orf95  
Affinity Capture-MS Homo sapiens
57 Cacybp 12301
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
58 Fbxw2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
59 Eed  
Affinity Capture-MS Mus musculus
60 Fbxw7  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
61 Cul4a  
Co-fractionation Mus musculus
62 Cops4 26891
Co-fractionation Mus musculus
63 RAE1 8480
Affinity Capture-MS Homo sapiens
64 Fbxw11  
Reconstituted Complex Mus musculus
65 Fancd2  
Affinity Capture-MS Mus musculus
66 Fbxl21  
PCA Mus musculus
67 PSMB2 5690
Affinity Capture-MS Homo sapiens
68 FBXL15  
Affinity Capture-MS Homo sapiens
69 Btrc  
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
70 FBXW11  
Affinity Capture-MS Homo sapiens
71 Zbtb7b  
Affinity Capture-MS Mus musculus
72 Ccdc22  
Co-fractionation Mus musculus
73 Fbxo3  
Co-fractionation Mus musculus
74 FBXL16  
Affinity Capture-MS Homo sapiens
75 FBXO21  
Affinity Capture-MS Homo sapiens
76 Ttc9c  
Co-fractionation Mus musculus
77 PSMA1 5682
Affinity Capture-MS Homo sapiens
78 Mettl21e  
Affinity Capture-MS Mus musculus
79 Fzr1  
Affinity Capture-MS Mus musculus
80 FBXO30 84085
Affinity Capture-MS Homo sapiens
81 Cpt2  
Co-fractionation Mus musculus
82 FBXO9 26268
Affinity Capture-MS Homo sapiens
83 FBXL18 80028
Affinity Capture-MS Homo sapiens
84 Rnf2  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
85 FBXW9  
Affinity Capture-MS Homo sapiens
86 KDM2B 84678
Affinity Capture-MS Homo sapiens
87 UTP6  
Affinity Capture-MS Homo sapiens
88 Skp2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
89 MRPS9 64965
Affinity Capture-MS Homo sapiens
90 Gps1 209318
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Skp1a is involved
PathwayEvidenceSource
Activation of NF-kappaB in B cells IEA Reactome
Adaptive Immune System IEA Reactome
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome
APC/C-mediated degradation of cell cycle proteins IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to chemical stress IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Class I MHC mediated antigen processing & presentation IEA Reactome
CLEC7A (Dectin-1) signaling IEA Reactome
Cyclin A:Cdk2-associated events at S phase entry IEA Reactome
Cyclin D associated events in G1 IEA Reactome
Cyclin E associated events during G1/S transition IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Dectin-1 mediated noncanonical NF-kB signaling IEA Reactome
Degradation of beta-catenin by the destruction complex IEA Reactome
Degradation of GLI1 by the proteasome IEA Reactome
DNA Replication IEA Reactome
Downstream signaling events of B Cell Receptor (BCR) IEA Reactome
Downstream TCR signaling IEA Reactome
FBXL7 down-regulates AURKA during mitotic entry and in early mitosis IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated NF-kB activation IEA Reactome
G1 Phase IEA Reactome
G1/S Transition IEA Reactome
G2/M Transition IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
GLI3 is processed to GLI3R by the proteasome IEA Reactome
GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 IEA Reactome
Hedgehog 'off' state IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Interleukin-1 family signaling IEA Reactome
Interleukin-1 signaling IEA Reactome
Interleukin-17 signaling IEA Reactome
Iron uptake and transport IEA Reactome
KEAP1-NFE2L2 pathway IEA Reactome
MAP kinase activation IEA Reactome
MAP3K8 (TPL2)-dependent MAPK1/3 activation IEA Reactome
Metabolism of proteins IEA Reactome
Mitotic G1 phase and G1/S transition IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
MyD88 cascade initiated on plasma membrane IEA Reactome
MyD88 dependent cascade initiated on endosome IEA Reactome
MyD88-independent TLR4 cascade IEA Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane IEA Reactome
Neddylation IEA Reactome
NIK-->noncanonical NF-kB signaling IEA Reactome
Nuclear events mediated by NFE2L2 IEA Reactome
Orc1 removal from chromatin IEA Reactome
Post-translational protein modification IEA Reactome
Prolactin receptor signaling IEA Reactome
Regulation of APC/C activators between G1/S and early anaphase IEA Reactome
Regulation of BACH1 activity IEA Reactome
Regulation of mitotic cell cycle IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Regulation of RUNX2 expression and activity IEA Reactome
RNA Polymerase II Transcription IEA Reactome
S Phase IEA Reactome
SCF(Skp2)-mediated degradation of p27/p21 IEA Reactome
SCF-beta-TrCP mediated degradation of Emi1 IEA Reactome
Signal Transduction IEA Reactome
Signaling by Hedgehog IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by the B Cell Receptor (BCR) IEA Reactome
Signaling by WNT IEA Reactome
Switching of origins to a post-replicative state IEA Reactome
Synthesis of DNA IEA Reactome
TCR signaling IEA Reactome
TNFR2 non-canonical NF-kB pathway IEA Reactome
Toll Like Receptor 10 (TLR10) Cascade IEA Reactome
Toll Like Receptor 2 (TLR2) Cascade IEA Reactome
Toll Like Receptor 3 (TLR3) Cascade IEA Reactome
Toll Like Receptor 4 (TLR4) Cascade IEA Reactome
Toll Like Receptor 5 (TLR5) Cascade IEA Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade IEA Reactome
Toll Like Receptor 9 (TLR9) Cascade IEA Reactome
Toll Like Receptor TLR1:TLR2 Cascade IEA Reactome
Toll Like Receptor TLR6:TLR2 Cascade IEA Reactome
Toll-like Receptor Cascades IEA Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation IEA Reactome
Transcriptional regulation by RUNX2 IEA Reactome
Transport of small molecules IEA Reactome
TRIF (TICAM1)-mediated TLR4 signaling IEA Reactome





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