Gene description for Prps2
Gene name phosphoribosyl pyrophosphate synthetase 2
Gene symbol Prps2
Other names/aliases 2610101M19Rik
AA589463
AI464149
Prps-2
Species Mus musculus
 Database cross references - Prps2
ExoCarta ExoCarta_110639
Vesiclepedia VP_110639
Entrez Gene 110639
UniProt Q9CS42  
 Prps2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prps2
Molecular Function
    magnesium ion binding GO:0000287 IEA
    magnesium ion binding GO:0000287 ISO
    ribose phosphate diphosphokinase activity GO:0004749 IBA
    ribose phosphate diphosphokinase activity GO:0004749 IDA
    ribose phosphate diphosphokinase activity GO:0004749 IMP
    ribose phosphate diphosphokinase activity GO:0004749 ISO
    ribose phosphate diphosphokinase activity GO:0004749 ISS
    ATP binding GO:0005524 ISO
    ATP binding GO:0005524 ISS
    AMP binding GO:0016208 ISO
    kinase activity GO:0016301 IEA
    GDP binding GO:0019003 ISO
    carbohydrate binding GO:0030246 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISS
    ADP binding GO:0043531 ISO
Biological Process
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 IBA
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 IDA
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 IEA
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 IMP
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 ISO
    pentose-phosphate shunt GO:0006098 IMP
    purine nucleotide biosynthetic process GO:0006164 IBA
    AMP biosynthetic process GO:0006167 ISO
    ribonucleoside monophosphate biosynthetic process GO:0009156 IEA
    nucleotide biosynthetic process GO:0009165 IEA
Subcellular Localization
    ribose phosphate diphosphokinase complex GO:0002189 ISO
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Prps2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prps2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Elp2  
Co-fractionation Mus musculus
2 Wdr12  
Co-fractionation Mus musculus
3 Zc3h15 69082
Co-fractionation Mus musculus
4 Rbm8a  
Co-fractionation Mus musculus
5 Atxn1  
Affinity Capture-MS Mus musculus
6 Ddx47  
Co-fractionation Mus musculus
7 Usp7  
Affinity Capture-MS Mus musculus
8 Rsl1d1 66409
Co-fractionation Mus musculus
9 Dkc1 245474
Co-fractionation Mus musculus
10 Bccip  
Co-fractionation Mus musculus
11 Trmt11  
Co-fractionation Mus musculus
12 Ada  
Co-fractionation Mus musculus
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