Gene description for Ppp2r4
Gene name protein phosphatase 2A activator, regulatory subunit B
Gene symbol Ppp2r4
Other names/aliases 2610042B21Rik
C77440
N28142
PR53
Species Mus musculus
 Database cross references - Ppp2r4
ExoCarta ExoCarta_110854
Vesiclepedia VP_110854
Entrez Gene 110854
UniProt P58389  
 Ppp2r4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ppp2r4
Molecular Function
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IBA
    phosphoprotein phosphatase activity GO:0004721 ISO
    phosphoprotein phosphatase activity GO:0004721 ISS
    signaling receptor binding GO:0005102 IEA
    signaling receptor binding GO:0005102 ISO
    ATP binding GO:0005524 ISO
    ATP binding GO:0005524 ISS
    protein tyrosine phosphatase activator activity GO:0008160 IBA
    protein tyrosine phosphatase activator activity GO:0008160 ISO
    protein tyrosine phosphatase activator activity GO:0008160 ISS
    protein phosphatase regulator activity GO:0019888 ISO
    protein phosphatase regulator activity GO:0019888 ISS
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    metal ion binding GO:0046872 IEA
    protein phosphatase 2A binding GO:0051721 ISO
    protein phosphatase 2A binding GO:0051721 ISS
    RNA polymerase II CTD heptapeptide repeat P3 isomerase activity GO:0140839 IEA
    RNA polymerase II CTD heptapeptide repeat P6 isomerase activity GO:0140840 IEA
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    mitotic spindle organization GO:0007052 IBA
    positive regulation of apoptotic process GO:0043065 IEA
    positive regulation of apoptotic process GO:0043065 ISO
Subcellular Localization
    protein phosphatase type 2A complex GO:0000159 IBA
    protein phosphatase type 2A complex GO:0000159 ISO
    protein phosphatase type 2A complex GO:0000159 ISS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    calcium channel complex GO:0034704 IEA
    calcium channel complex GO:0034704 ISO
    ATPase complex GO:1904949 ISO
    ATPase complex GO:1904949 ISS
 Experiment description of studies that identified Ppp2r4 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ppp2r4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ppp1ca 19045
Affinity Capture-Western Mus musculus
2 Ppp2r5b  
Co-fractionation Mus musculus
3 Ppp2ca 19052
Affinity Capture-Western Mus musculus
4 Strn3  
Co-fractionation Mus musculus
5 Ppp2r5c 26931
Co-fractionation Mus musculus
6 Igbp1  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Ppp2r4 is involved
No pathways found





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