Gene description for MAP4K5
Gene name mitogen-activated protein kinase kinase kinase kinase 5
Gene symbol MAP4K5
Other names/aliases GCKR
KHS
KHS1
MAPKKKK5
Species Homo sapiens
 Database cross references - MAP4K5
ExoCarta ExoCarta_11183
Vesiclepedia VP_11183
Entrez Gene 11183
HGNC 6867
MIM 604923
UniProt Q9Y4K4  
 MAP4K5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MAP4K5
Molecular Function
    protein kinase activity GO:0004672 TAS
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    MAP kinase kinase kinase kinase activity GO:0008349 IBA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    MAPK cascade GO:0000165 IEA
    chromatin remodeling GO:0006338 IEA
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified MAP4K5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAP4K5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF1AD 84285
Affinity Capture-MS Homo sapiens
2 RAB35 11021
Proximity Label-MS Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 TRAF2 7186
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 EBAG9 9166
Proximity Label-MS Homo sapiens
6 NEDD4 4734
Reconstituted Complex Homo sapiens
7 GRB2 2885
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 LAMTOR1 55004
Proximity Label-MS Homo sapiens
9 GJA1 2697
Proximity Label-MS Homo sapiens
10 PPM1B 5495
Co-fractionation Homo sapiens
11 CRK 1398
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 SMURF1 57154
Biochemical Activity Homo sapiens
13 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
14 ARF6 382
Proximity Label-MS Homo sapiens
15 HCK 3055
Two-hybrid Homo sapiens
16 CXADR 1525
Proximity Label-MS Homo sapiens
17 MAP4K3 8491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 C11orf52 91894
Proximity Label-MS Homo sapiens
19 SETDB1  
Two-hybrid Homo sapiens
20 CDRT15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 LYN 4067
Proximity Label-MS Homo sapiens
22 POU2F1 5451
Co-fractionation Homo sapiens
23 MCAM 4162
Proximity Label-MS Homo sapiens
24 PRKACA 5566
Proximity Label-MS Homo sapiens
25 DNAJC5 80331
Proximity Label-MS Homo sapiens
26 GTF2H1 2965
Co-fractionation Homo sapiens
27 RPA3 6119
Proximity Label-MS Homo sapiens
28 DIRAS3  
Proximity Label-MS Homo sapiens
29 NTRK1 4914
Affinity Capture-MS Homo sapiens
30 CRKL 1399
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
31 RAB11A 8766
Proximity Label-MS Homo sapiens
32 NAGA 4668
Co-fractionation Homo sapiens
33 COPS5 10987
Two-hybrid Homo sapiens
34 EPHA2 1969
Proximity Label-MS Homo sapiens
35 KRAS 3845
Proximity Label-MS Homo sapiens
36 RHOB 388
Proximity Label-MS Homo sapiens
37 ZNF440  
Two-hybrid Homo sapiens
38 MAD2L1 4085
Affinity Capture-MS Homo sapiens
39 CAV1 857
Proximity Label-MS Homo sapiens
40 AKR1A1 10327
Affinity Capture-MS Homo sapiens
41 OCLN 100506658
Proximity Label-MS Homo sapiens
42 PPM1A 5494
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which MAP4K5 is involved
No pathways found





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