Gene description for Gne
Gene name glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Gene symbol Gne
Other names/aliases Uae1
Species Rattus norvegicus
 Database cross references - Gne
ExoCarta ExoCarta_114711
Vesiclepedia VP_114711
Entrez Gene 114711
UniProt O35826  
 Gne identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Gne
Molecular Function
    hydrolase activity, hydrolyzing O-glycosyl compounds GO:0004553 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    UDP-N-acetylglucosamine 2-epimerase activity GO:0008761 IBA
    UDP-N-acetylglucosamine 2-epimerase activity GO:0008761 IDA
    UDP-N-acetylglucosamine 2-epimerase activity GO:0008761 IEA
    UDP-N-acetylglucosamine 2-epimerase activity GO:0008761 ISO
    N-acylmannosamine kinase activity GO:0009384 IBA
    N-acylmannosamine kinase activity GO:0009384 IDA
    N-acylmannosamine kinase activity GO:0009384 IEA
    N-acylmannosamine kinase activity GO:0009384 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    N-acetylglucosamine biosynthetic process GO:0006045 IEA
    UDP-N-acetylglucosamine metabolic process GO:0006047 IEA
    CMP-N-acetylneuraminate biosynthetic process GO:0006055 IEA
    CMP-N-acetylneuraminate biosynthetic process GO:0006055 ISO
    N-acetylneuraminate biosynthetic process GO:0046380 IC
    N-acetylneuraminate biosynthetic process GO:0046380 IEA
    N-acetylneuraminate biosynthetic process GO:0046380 ISO
    N-acetylneuraminate biosynthetic process GO:0046380 TAS
    carbohydrate phosphorylation GO:0046835 IEA
    glycosylation GO:0070085 IEA
    glycosylation GO:0070085 ISO
Subcellular Localization
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Gne in sEVs
1
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Gne
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZBTB16  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 KIAA1549  
Two-hybrid Homo sapiens
3 CRMP1 1400
Two-hybrid Homo sapiens
4 Crmp1  
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml



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