Gene description for CRMP1
Gene name collapsin response mediator protein 1
Gene symbol CRMP1
Other names/aliases CRMP-1
DPYSL1
DRP-1
DRP1
ULIP-3
Species Homo sapiens
 Database cross references - CRMP1
ExoCarta ExoCarta_1400
Vesiclepedia VP_1400
Entrez Gene 1400
HGNC 2365
MIM 602462
UniProt Q14194  
 CRMP1 identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for CRMP1
Molecular Function
    dihydropyrimidinase activity GO:0004157 IBA
    protein binding GO:0005515 IPI
    hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides GO:0016812 IBA
    filamin binding GO:0031005 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    nucleobase-containing compound metabolic process GO:0006139 TAS
    pyrimidine nucleobase catabolic process GO:0006208 IBA
    nervous system development GO:0007399 TAS
    negative regulation of neuron projection development GO:0010977 IGI
Subcellular Localization
    centrosome GO:0005813 IDA
    spindle GO:0005819 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    growth cone GO:0030426 IEA
    midbody GO:0030496 IDA
    perikaryon GO:0043204 IEA
 Experiment description of studies that identified CRMP1 in sEVs
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for CRMP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Two-hybrid Homo sapiens
2 ATP6V1B2 526
Co-fractionation Homo sapiens
3 RPS6KA5 9252
Two-hybrid Homo sapiens
4 ZNF24  
Two-hybrid Homo sapiens
5 SNRPG 6637
Two-hybrid Homo sapiens
6 UBE2A  
Two-hybrid Homo sapiens
7 TK1 7083
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 GPM6A 2823
Affinity Capture-MS Homo sapiens
9 PSMD11 5717
Two-hybrid Homo sapiens
10 RPA2 6118
Two-hybrid Homo sapiens
11 ARL15 54622
Two-hybrid Homo sapiens
12 ALDH2 217
Two-hybrid Homo sapiens
13 PAGE2B  
Affinity Capture-MS Homo sapiens
14 RTN4 57142
Two-hybrid Homo sapiens
15 PMF1  
Two-hybrid Homo sapiens
16 CCL18  
Two-hybrid Homo sapiens
17 CORO1B 57175
Co-fractionation Homo sapiens
18 LRRC1 55227
Two-hybrid Homo sapiens
19 DPYSL2 1808
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
20 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SEC24C 9632
Co-fractionation Homo sapiens
22 ZAK 51776
Two-hybrid Homo sapiens
23 HGS 9146
Reconstituted Complex Homo sapiens
24 NAT9  
Two-hybrid Homo sapiens
25 PARVA 55742
Co-fractionation Homo sapiens
26 SRC 6714
Two-hybrid Homo sapiens
27 RHOA 387
Phenotypic Suppression Homo sapiens
28 TOM1 10043
Affinity Capture-MS Homo sapiens
29 CRMP1 1400
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 KLHL20  
Two-hybrid Homo sapiens
31 MECP2 4204
Affinity Capture-MS Homo sapiens
32 CUL1 8454
Co-fractionation Homo sapiens
33 RRP7A 27341
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 MAPK8IP2  
Two-hybrid Homo sapiens
35 FAS 355
Two-hybrid Homo sapiens
36 TUBB 615087
Affinity Capture-Western Bos taurus
37 LSM2 57819
Two-hybrid Homo sapiens
38 SERPINB9 5272
Two-hybrid Homo sapiens
39 PFN1 5216
Two-hybrid Homo sapiens
40 AP3M1 26985
Two-hybrid Homo sapiens
41 ANXA7 310
Two-hybrid Homo sapiens
42 SEPHS1 22929
Two-hybrid Homo sapiens
43 Gne 114711
Two-hybrid Rattus norvegicus
44 DPYSL4 10570
Affinity Capture-MS Homo sapiens
45 RGS2  
Two-hybrid Homo sapiens
46 MOB4 25843
Two-hybrid Homo sapiens
47 AMFR 267
Two-hybrid Homo sapiens
48 TUBA1A 404053
Affinity Capture-Western Bos taurus
49 GOLGA2 2801
Two-hybrid Homo sapiens
50 HTT 3064
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
51 SREBF2 6721
Negative Genetic Homo sapiens
52 FUBP1 8880
Two-hybrid Homo sapiens
53 SWAP70 23075
Co-fractionation Homo sapiens
54 VCP 7415
Two-hybrid Homo sapiens
55 NTRK1 4914
Affinity Capture-MS Homo sapiens
56 ARMC1 55156
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 AGR2 10551
Two-hybrid Homo sapiens
58 EIF4EBP1  
Affinity Capture-MS Homo sapiens
59 SAT1  
Two-hybrid Homo sapiens
60 DNAJB11 51726
Two-hybrid Homo sapiens
61 ARFGAP1 55738
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 EEF1D 1936
Two-hybrid Homo sapiens
63 TRIP13 9319
Two-hybrid Homo sapiens
64 DISC1 27185
Reconstituted Complex Homo sapiens
65 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 CNN3 1266
Affinity Capture-MS Homo sapiens
67 CACNA1A  
Two-hybrid Homo sapiens
68 YAE1D1  
Two-hybrid Homo sapiens
69 MRPS12  
Two-hybrid Homo sapiens
70 CDCA8 55143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 PITHD1 57095
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 FTH1 2495
Two-hybrid Homo sapiens
73 HNRNPUL1 11100
Two-hybrid Homo sapiens
74 POLR2A 5430
Affinity Capture-MS Homo sapiens
75 TFG 10342
Two-hybrid Homo sapiens
76 CDK5RAP2 55755
Two-hybrid Homo sapiens
77 EPN1 29924
Two-hybrid Homo sapiens
78 HSPA4 3308
Co-fractionation Homo sapiens
79 EIF2S2 8894
Two-hybrid Homo sapiens
80 HAX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 NDUFV2 4729
Two-hybrid Homo sapiens
82 DPYSL3 1809
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
83 HSPE1 3336
Two-hybrid Homo sapiens
84 PPT1 5538
Affinity Capture-MS Homo sapiens
85 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
86 SPRY2 10253
Two-hybrid Homo sapiens
87 RGL2 5863
Two-hybrid Homo sapiens
88 PAFAH1B3 5050
Two-hybrid Homo sapiens
89 HMGB1 3146
Two-hybrid Homo sapiens
90 UBA5 79876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 FXR1 8087
Two-hybrid Homo sapiens
92 TTLL6  
Affinity Capture-MS Homo sapiens
93 BID  
Two-hybrid Homo sapiens
94 PPP1R8 5511
Two-hybrid Homo sapiens
95 CCDC106  
Two-hybrid Homo sapiens
96 AXIN1  
Two-hybrid Homo sapiens
97 OTUD6B 51633
Co-fractionation Homo sapiens
98 CCT7 10574
Two-hybrid Homo sapiens
99 XAGE3  
Affinity Capture-MS Homo sapiens
100 BTBD2  
Two-hybrid Homo sapiens
101 TSC22D1 8848
Two-hybrid Homo sapiens
102 UBE2B 7320
Two-hybrid Homo sapiens
103 HNRNPH3 3189
Two-hybrid Homo sapiens
104 DNAJB4 11080
Co-fractionation Homo sapiens
105 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 CDK5RAP3 80279
Two-hybrid Homo sapiens
107 DUSP4 1846
Two-hybrid Homo sapiens
108 SERBP1 26135
Affinity Capture-MS Homo sapiens
109 VIM 7431
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
110 NVL  
Two-hybrid Homo sapiens
111 JMJD6 23210
Co-fractionation Homo sapiens
112 EXOSC8  
Two-hybrid Homo sapiens
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