Gene description for Apaf1
Gene name apoptotic peptidase activating factor 1
Gene symbol Apaf1
Other names/aliases 6230400I06Rik
Apaf-1
Apaf1l
fog
mKIAA0413
Species Mus musculus
 Database cross references - Apaf1
ExoCarta ExoCarta_11783
Vesiclepedia VP_11783
Entrez Gene 11783
UniProt O88879  
 Apaf1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Apaf1
Molecular Function
    ATP binding GO:0005524 IEA
    cysteine-type endopeptidase activator activity involved in apoptotic process GO:0008656 IDA
    heat shock protein binding GO:0031072 IEA
    heat shock protein binding GO:0031072 ISO
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    ADP binding GO:0043531 IEA
Biological Process
    response to hypoxia GO:0001666 IEA
    kidney development GO:0001822 IEA
    neural tube closure GO:0001843 IMP
    multicellular organism development GO:0007275 IMP
    brain development GO:0007420 IMP
    response to nutrient GO:0007584 IEA
    cardiac muscle cell apoptotic process GO:0010659 IEA
    cardiac muscle cell apoptotic process GO:0010659 ISO
    cell differentiation GO:0030154 IEA
    forebrain development GO:0030900 IMP
    neuron apoptotic process GO:0051402 IGI
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 IMP
    cellular response to transforming growth factor beta stimulus GO:0071560 IEA
    cellular response to transforming growth factor beta stimulus GO:0071560 ISO
    intrinsic apoptotic signaling pathway GO:0097193 NAS
    regulation of apoptotic DNA fragmentation GO:1902510 IGI
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IGI
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IMP
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
    positive regulation of apoptotic signaling pathway GO:2001235 IGI
Subcellular Localization
    nucleus GO:0005634 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    protein-containing complex GO:0032991 ISO
    apoptosome GO:0043293 ISO
 Experiment description of studies that identified Apaf1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Apaf1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ppp1ca 19045
Affinity Capture-Western Mus musculus
2 Casp8 12370
Co-fractionation Mus musculus
3 Tnfrsf23  
Co-fractionation Mus musculus
View the network image/svg+xml



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