Gene description for Casp8
Gene name caspase 8
Gene symbol Casp8
Other names/aliases CASP-8
FLICE
MACH
Mch5
Species Mus musculus
 Database cross references - Casp8
ExoCarta ExoCarta_12370
Vesiclepedia VP_12370
Entrez Gene 12370
UniProt O89110  
 Casp8 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Casp8
Molecular Function
    endopeptidase activity GO:0004175 IDA
    endopeptidase activity GO:0004175 IMP
    cysteine-type endopeptidase activity GO:0004197 IBA
    cysteine-type endopeptidase activity GO:0004197 IDA
    cysteine-type endopeptidase activity GO:0004197 IGI
    cysteine-type endopeptidase activity GO:0004197 IMP
    cysteine-type endopeptidase activity GO:0004197 ISO
    death receptor binding GO:0005123 ISO
    tumor necrosis factor receptor binding GO:0005164 ISO
    protein binding GO:0005515 IPI
    peptidase activity GO:0008233 IDA
    peptidase activity GO:0008233 IMP
    peptidase activity GO:0008233 ISO
    cysteine-type endopeptidase activator activity involved in apoptotic process GO:0008656 IDA
    ubiquitin protein ligase binding GO:0031625 ISO
    death effector domain binding GO:0035877 ISO
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 ISO
    scaffold protein binding GO:0097110 ISO
Biological Process
    angiogenesis GO:0001525 IMP
    angiogenesis GO:0001525 IMP
    regulation of cytokine production GO:0001817 IDA
    neural tube formation GO:0001841 IMP
    proteolysis GO:0006508 ISO
    apoptotic process GO:0006915 IBA
    apoptotic process GO:0006915 IDA
    apoptotic process GO:0006915 ISO
    apoptotic process GO:0006915 ISO
    heart development GO:0007507 IMP
    heart development GO:0007507 IMP
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IBA
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IMP
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 TAS
    protein processing GO:0016485 IDA
    protein processing GO:0016485 ISO
    macrophage differentiation GO:0030225 IBA
    macrophage differentiation GO:0030225 IMP
    macrophage differentiation GO:0030225 TAS
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of interleukin-1 beta production GO:0032731 ISO
    response to tumor necrosis factor GO:0034612 ISO
    TRAIL-activated apoptotic signaling pathway GO:0036462 ISO
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of apoptotic process GO:0043065 IMP
    positive regulation of apoptotic process GO:0043065 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISO
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 ISO
    positive regulation of neuron apoptotic process GO:0043525 IBA
    positive regulation of neuron apoptotic process GO:0043525 ISO
    regulation of innate immune response GO:0045088 IDA
    response to ethanol GO:0045471 ISO
    positive regulation of macrophage differentiation GO:0045651 ISO
    positive regulation of proteolysis GO:0045862 ISO
    cardiac muscle tissue development GO:0048738 TAS
    proteolysis involved in protein catabolic process GO:0051603 IDA
    proteolysis involved in protein catabolic process GO:0051603 ISO
    protein maturation GO:0051604 ISO
    negative regulation of necroptotic process GO:0060546 IGI
    negative regulation of necroptotic process GO:0060546 IMP
    negative regulation of necroptotic process GO:0060546 ISO
    regulation of thymocyte apoptotic process GO:0070243 IGI
    necroptotic process GO:0070266 IGI
    pyroptotic inflammatory response GO:0070269 IDA
    pyroptotic inflammatory response GO:0070269 IMP
    apoptotic signaling pathway GO:0097190 IDA
    apoptotic signaling pathway GO:0097190 ISO
    extrinsic apoptotic signaling pathway GO:0097191 IDA
    extrinsic apoptotic signaling pathway GO:0097191 IGI
    extrinsic apoptotic signaling pathway GO:0097191 IMP
    extrinsic apoptotic signaling pathway GO:0097191 ISO
    execution phase of apoptosis GO:0097194 IMP
    execution phase of apoptosis GO:0097194 ISO
    execution phase of apoptosis GO:0097194 ISS
    activation of cysteine-type endopeptidase activity GO:0097202 ISO
    self proteolysis GO:0097264 IMP
    hepatocyte apoptotic process GO:0097284 IMP
    positive regulation of execution phase of apoptosis GO:1900119 IDA
    positive regulation of execution phase of apoptosis GO:1900119 IMP
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IDA
    regulation of apoptotic signaling pathway GO:2001233 IGI
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IGI
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IDA
    lamellipodium GO:0030027 ISO
    Noc1p-Noc2p complex GO:0030690 IMP
    death-inducing signaling complex GO:0031264 ISO
    CD95 death-inducing signaling complex GO:0031265 IBA
    CD95 death-inducing signaling complex GO:0031265 ISO
    protein-containing complex GO:0032991 ISO
    cell body GO:0044297 ISO
    ripoptosome GO:0097342 ISO
    ripoptosome GO:0097342 ISS
 Experiment description of studies that identified Casp8 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Casp8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fas 14102
Affinity Capture-Western Mus musculus
2 Bnip3l  
Co-fractionation Mus musculus
3 Il1b  
Affinity Capture-Western Mus musculus
4 Fadd  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
5 Sqstm1  
Affinity Capture-Western Mus musculus
Co-localization Mus musculus
Affinity Capture-Western Mus musculus
6 Bfar  
Co-fractionation Mus musculus
7 Nod1  
Co-fractionation Mus musculus
8 Cradd  
Co-fractionation Mus musculus
9 Usp7  
Affinity Capture-MS Mus musculus
10 Tnfrsf23  
Co-fractionation Mus musculus
11 Fitm2  
Positive Genetic Mus musculus
12 Cyld  
Biochemical Activity Mus musculus
13 Atg5  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
14 Casp8ap2  
Co-fractionation Mus musculus
15 Pcyt1a  
Co-fractionation Mus musculus
16 Apaf1 11783
Co-fractionation Mus musculus
17 Atg12  
Positive Genetic Mus musculus
View the network image/svg+xml
 Pathways in which Casp8 is involved
PathwayEvidenceSource
Activation, myristolyation of BID and translocation to mitochondria IEA Reactome
Apoptosis IEA Reactome
Apoptotic cleavage of cellular proteins IEA Reactome
Apoptotic execution phase IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
CASP8 activity is inhibited IEA Reactome
Caspase activation via Death Receptors in the presence of ligand IEA Reactome
Caspase activation via extrinsic apoptotic signalling pathway IEA Reactome
Caspase-mediated cleavage of cytoskeletal proteins IEA Reactome
CLEC7A (Dectin-1) signaling IEA Reactome
CLEC7A/inflammasome pathway IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Death Receptor Signaling IEA Reactome
Dimerization of procaspase-8 IEA Reactome
FasL/ CD95L signaling IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Interleukin-1 family signaling IEA Reactome
Interleukin-1 signaling IEA Reactome
Intrinsic Pathway for Apoptosis IEA Reactome
MyD88 cascade initiated on plasma membrane IEA Reactome
MyD88 dependent cascade initiated on endosome IEA Reactome
MyD88-independent TLR4 cascade IEA Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane IEA Reactome
NOD1/2 Signaling Pathway IEA Reactome
Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways IEA Reactome
Programmed Cell Death IEA Reactome
Regulated Necrosis IEA Reactome
Regulation by c-FLIP IEA Reactome
Regulation of necroptotic cell death IEA Reactome
Regulation of NF-kappa B signaling IEA Reactome
Regulation of TNFR1 signaling IEA Reactome
RIPK1-mediated regulated necrosis IEA Reactome
Signal Transduction IEA Reactome
Signaling by Interleukins IEA Reactome
TAK1-dependent IKK and NF-kappa-B activation IEA Reactome
TNF signaling IEA Reactome
Toll Like Receptor 10 (TLR10) Cascade IEA Reactome
Toll Like Receptor 2 (TLR2) Cascade IEA Reactome
Toll Like Receptor 3 (TLR3) Cascade IEA Reactome
Toll Like Receptor 4 (TLR4) Cascade IEA Reactome
Toll Like Receptor 5 (TLR5) Cascade IEA Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade IEA Reactome
Toll Like Receptor 9 (TLR9) Cascade IEA Reactome
Toll Like Receptor TLR1:TLR2 Cascade IEA Reactome
Toll Like Receptor TLR6:TLR2 Cascade IEA Reactome
Toll-like Receptor Cascades IEA Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation IEA Reactome
TRAIL signaling IEA Reactome
TRIF (TICAM1)-mediated TLR4 signaling IEA Reactome
TRIF-mediated programmed cell death IEA Reactome





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