Gene description for Col1a1
Gene name collagen, type I, alpha 1
Gene symbol Col1a1
Other names/aliases Col1a-1
Cola-1
Cola1
Mov-13
Mov13
Species Mus musculus
 Database cross references - Col1a1
ExoCarta ExoCarta_12842
Vesiclepedia VP_12842
Entrez Gene 12842
UniProt P11087  
 Col1a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Col1a1
Molecular Function
    protease binding GO:0002020 IEA
    protease binding GO:0002020 ISO
    extracellular matrix structural constituent GO:0005201 IDA
    protein binding GO:0005515 IPI
    extracellular matrix structural constituent conferring tensile strength GO:0030020 IBA
    extracellular matrix structural constituent conferring tensile strength GO:0030020 RCA
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
    platelet-derived growth factor binding GO:0048407 ISO
Biological Process
    skeletal system development GO:0001501 IBA
    skeletal system development GO:0001501 IMP
    skeletal system development GO:0001501 ISO
    skeletal system development GO:0001501 ISO
    ossification GO:0001503 IBA
    blood vessel development GO:0001568 IBA
    blood vessel development GO:0001568 IMP
    blood vessel development GO:0001568 ISO
    osteoblast differentiation GO:0001649 IEP
    intramembranous ossification GO:0001957 IGI
    endochondral ossification GO:0001958 IMP
    visual perception GO:0007601 IEA
    visual perception GO:0007601 ISO
    sensory perception of sound GO:0007605 IEA
    sensory perception of sound GO:0007605 ISO
    response to xenobiotic stimulus GO:0009410 IEA
    response to mechanical stimulus GO:0009612 IBA
    positive regulation of epithelial to mesenchymal transition GO:0010718 IEA
    positive regulation of epithelial to mesenchymal transition GO:0010718 ISO
    negative regulation of cell-substrate adhesion GO:0010812 IDA
    protein transport GO:0015031 IMP
    extracellular matrix organization GO:0030198 IBA
    collagen fibril organization GO:0030199 ISO
    collagen fibril organization GO:0030199 NAS
    positive regulation of cell migration GO:0030335 IEA
    positive regulation of cell migration GO:0030335 ISO
    response to estradiol GO:0032355 IEA
    response to insulin GO:0032868 IEA
    collagen biosynthetic process GO:0032964 IEA
    collagen biosynthetic process GO:0032964 ISO
    protein localization to nucleus GO:0034504 IEA
    protein localization to nucleus GO:0034504 ISO
    tooth mineralization GO:0034505 IEA
    tooth mineralization GO:0034505 ISO
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 ISO
    response to hydrogen peroxide GO:0042542 IEA
    skin development GO:0043588 IBA
    skin development GO:0043588 IMP
    skin morphogenesis GO:0043589 IEA
    skin morphogenesis GO:0043589 ISO
    cellular response to fibroblast growth factor stimulus GO:0044344 IEA
    positive regulation of DNA-templated transcription GO:0045893 IEA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    response to steroid hormone GO:0048545 IEA
    skeletal system morphogenesis GO:0048705 IGI
    embryonic skeletal system development GO:0048706 IEA
    embryonic skeletal system development GO:0048706 ISO
    response to cAMP GO:0051591 IEA
    response to hyperoxia GO:0055093 IEA
    face morphogenesis GO:0060325 IGI
    bone trabecula formation GO:0060346 IGI
    cartilage development involved in endochondral bone morphogenesis GO:0060351 IMP
    cellular response to amino acid stimulus GO:0071230 IDA
    cellular response to mechanical stimulus GO:0071260 IEP
    cellular response to retinoic acid GO:0071300 IEA
    cellular response to vitamin E GO:0071306 IEA
    cellular response to glucose stimulus GO:0071333 IEA
    cellular response to tumor necrosis factor GO:0071356 IEA
    cellular response to epidermal growth factor stimulus GO:0071364 IEA
    cellular response to transforming growth factor beta stimulus GO:0071560 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISO
    cellular response to fluoride GO:1902618 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    collagen trimer GO:0005581 IDA
    collagen trimer GO:0005581 IMP
    collagen type I trimer GO:0005584 IBA
    collagen type I trimer GO:0005584 IDA
    collagen type I trimer GO:0005584 IMP
    collagen type I trimer GO:0005584 ISO
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    secretory granule GO:0030141 IEA
    secretory granule GO:0030141 ISO
    extracellular matrix GO:0031012 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
    collagen-containing extracellular matrix GO:0062023 IDA
 Experiment description of studies that identified Col1a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Col1a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Smad4 17128
Affinity Capture-Western Mus musculus
2 Serpinf1 20317
Reconstituted Complex Mus musculus
3 Smad3  
Affinity Capture-Western Mus musculus
4 Smad7  
Affinity Capture-Western Mus musculus
5 Smad2  
Affinity Capture-Western Mus musculus
6 Tgfb1 21803
Affinity Capture-Western Mus musculus
7 Bmp1 12153
Affinity Capture-Western Mus musculus
8 Lum  
Co-fractionation Mus musculus
9 Kcnma1  
Affinity Capture-MS Mus musculus
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