Gene description for Smad4
Gene name	SMAD family member 4
Gene symbol	Smad4
Other names/aliases	AW743858 D18Wsu70e DPC4 Madh4
Species	Mus musculus
Database cross references - Smad4
ExoCarta	ExoCarta_17128
Vesiclepedia	VP_17128
Entrez Gene	17128
UniProt	P97471
Smad4 identified in sEVs derived from the following tissue/cell type
Mast cells	17486113

Gene ontology annotations for Smad4

Molecular Function     transcription cis-regulatory region binding GO:0000976 ISO     RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IDA     RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IGI     RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA     RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA     RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISO     DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA     transcription corepressor binding GO:0001222 IEA     transcription corepressor binding GO:0001222 ISO     transcription coactivator binding GO:0001223 IEA     transcription coactivator binding GO:0001223 ISO     DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA     DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IGI     DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 ISO     DNA binding GO:0003677 IDA     DNA binding GO:0003677 ISO     DNA binding GO:0003677 ISO     chromatin binding GO:0003682 IDA     DNA-binding transcription factor activity GO:0003700 IDA     DNA-binding transcription factor activity GO:0003700 ISO     DNA-binding transcription factor activity GO:0003700 ISO     protein binding GO:0005515 IPI     collagen binding GO:0005518 IPI     filamin binding GO:0031005 IEA     filamin binding GO:0031005 ISO     identical protein binding GO:0042802 IPI     identical protein binding GO:0042802 ISO     protein homodimerization activity GO:0042803 IEA     protein homodimerization activity GO:0042803 ISO     sulfate binding GO:0043199 IEA     sulfate binding GO:0043199 ISO     sequence-specific DNA binding GO:0043565 ISO     protein-containing complex binding GO:0044877 ISO     SMAD binding GO:0046332 ISO     metal ion binding GO:0046872 IEA     RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI     I-SMAD binding GO:0070411 IBA     I-SMAD binding GO:0070411 IEA     I-SMAD binding GO:0070411 ISO     R-SMAD binding GO:0070412 IEA     R-SMAD binding GO:0070412 ISO Biological Process     negative regulation of transcription by RNA polymerase II GO:0000122 IMP     negative regulation of transcription by RNA polymerase II GO:0000122 IMP     ovarian follicle development GO:0001541 IGI     ovarian follicle development GO:0001541 IMP     osteoblast differentiation GO:0001649 IEA     osteoblast differentiation GO:0001649 ISO     branching involved in ureteric bud morphogenesis GO:0001658 IGI     response to hypoxia GO:0001666 IEA     response to hypoxia GO:0001666 ISO     in utero embryonic development GO:0001701 IMP     gastrulation with mouth forming second GO:0001702 IMP     kidney development GO:0001822 IMP     epithelial to mesenchymal transition GO:0001837 ISO     outflow tract septum morphogenesis GO:0003148 IGI     atrioventricular valve formation GO:0003190 IGI     epithelial to mesenchymal transition involved in endocardial cushion formation GO:0003198 IMP     left ventricular cardiac muscle tissue morphogenesis GO:0003220 IMP     positive regulation of cell proliferation involved in heart valve morphogenesis GO:0003251 IMP     cardiac septum development GO:0003279 IGI     brainstem development GO:0003360 IMP     DNA-templated transcription GO:0006351 ISO     regulation of DNA-templated transcription GO:0006355 NAS     regulation of transcription by RNA polymerase II GO:0006357 IBA     regulation of transcription by RNA polymerase II GO:0006357 IDA     transcription by RNA polymerase II GO:0006366 IMP     intracellular iron ion homeostasis GO:0006879 IMP     transforming growth factor beta receptor signaling pathway GO:0007179 IBA     transforming growth factor beta receptor signaling pathway GO:0007179 IGI     transforming growth factor beta receptor signaling pathway GO:0007179 IMP     transforming growth factor beta receptor signaling pathway GO:0007179 ISO     transforming growth factor beta receptor signaling pathway GO:0007179 NAS     spermatogenesis GO:0007283 IMP     single fertilization GO:0007338 IGI     gastrulation GO:0007369 IMP     axon guidance GO:0007411 IMP     endoderm development GO:0007492 IMP     mesoderm development GO:0007498 IMP     cell population proliferation GO:0008283 IGI     cell population proliferation GO:0008283 IMP     negative regulation of cell population proliferation GO:0008285 IGI     male gonad development GO:0008584 IMP     female gonad development GO:0008585 IGI     anatomical structure morphogenesis GO:0009653 IBA     anatomical structure morphogenesis GO:0009653 IMP     anterior/posterior pattern specification GO:0009952 IMP     negative regulation of cardiac muscle hypertrophy GO:0010614 IMP     positive regulation of gene expression GO:0010628 IEA     positive regulation of gene expression GO:0010628 ISO     epithelial cell migration GO:0010631 IEA     epithelial cell migration GO:0010631 ISO     positive regulation of cardiac muscle cell apoptotic process GO:0010666 IEA     positive regulation of cardiac muscle cell apoptotic process GO:0010666 ISO     positive regulation of epithelial to mesenchymal transition GO:0010718 IMP     neural crest cell differentiation GO:0014033 IMP     cardiac muscle hypertrophy in response to stress GO:0014898 IEA     cardiac muscle hypertrophy in response to stress GO:0014898 ISO     cell differentiation GO:0030154 IBA     cell differentiation GO:0030154 IMP     negative regulation of cell growth GO:0030308 IEA     negative regulation of cell growth GO:0030308 ISO     adrenal gland development GO:0030325 IEA     BMP signaling pathway GO:0030509 IBA     BMP signaling pathway GO:0030509 IMP     BMP signaling pathway GO:0030509 ISO     positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 IEA     positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 ISO     somite rostral/caudal axis specification GO:0032525 IMP     regulation of transforming growth factor beta2 production GO:0032909 IEA     regulation of transforming growth factor beta2 production GO:0032909 ISO     activin receptor signaling pathway GO:0032924 NAS     positive regulation of luteinizing hormone secretion GO:0033686 IGI     positive regulation of luteinizing hormone secretion GO:0033686 IMP     intracellular signal transduction GO:0035556 ISO     atrioventricular canal development GO:0036302 IGI     endothelial cell activation GO:0042118 IMP     regulation of cell population proliferation GO:0042127 IGI     regulation of cell population proliferation GO:0042127 IMP     negative regulation of protein catabolic process GO:0042177 IEA     negative regulation of protein catabolic process GO:0042177 ISO     embryonic digit morphogenesis GO:0042733 IMP     negative regulation of DNA-templated transcription GO:0045892 ISO     positive regulation of DNA-templated transcription GO:0045893 IDA     positive regulation of DNA-templated transcription GO:0045893 ISO     positive regulation of transcription by RNA polymerase II GO:0045944 IDA     positive regulation of transcription by RNA polymerase II GO:0045944 IDA     positive regulation of transcription by RNA polymerase II GO:0045944 IGI     positive regulation of transcription by RNA polymerase II GO:0045944 IMP     positive regulation of transcription by RNA polymerase II GO:0045944 IMP     positive regulation of transcription by RNA polymerase II GO:0045944 ISO     positive regulation of follicle-stimulating hormone secretion GO:0046881 IGI     positive regulation of follicle-stimulating hormone secretion GO:0046881 IMP     mesendoderm development GO:0048382 IMP     developmental growth GO:0048589 IMP     neuron fate commitment GO:0048663 IMP     neuron fate specification GO:0048665 IEA     neuron fate specification GO:0048665 ISO     tissue morphogenesis GO:0048729 IMP     sebaceous gland development GO:0048733 IMP     formation of anatomical boundary GO:0048859 IMP     regulation of binding GO:0051098 IDA     regulation of hair follicle development GO:0051797 IMP     uterus development GO:0060065 IGI     positive regulation of SMAD protein signal transduction GO:0060391 IMP     SMAD protein signal transduction GO:0060395 IBA     SMAD protein signal transduction GO:0060395 IMP     SMAD protein signal transduction GO:0060395 IMP     SMAD protein signal transduction GO:0060395 ISO     ventricular septum morphogenesis GO:0060412 IMP     endocardial cell differentiation GO:0060956 IMP     female gonad morphogenesis GO:0061040 IMP     secondary palate development GO:0062009 IMP     interleukin-6-mediated signaling pathway GO:0070102 IDA     ERK1 and ERK2 cascade GO:0070371 IEA     ERK1 and ERK2 cascade GO:0070371 ISO     negative regulation of ERK1 and ERK2 cascade GO:0070373 IMP     cellular response to glucose stimulus GO:0071333 IEA     cellular response to glucose stimulus GO:0071333 ISO     response to transforming growth factor beta GO:0071559 ISO     cellular response to transforming growth factor beta stimulus GO:0071560 ISO     metanephric mesenchyme morphogenesis GO:0072133 IMP     nephrogenic mesenchyme morphogenesis GO:0072134 IMP     seminiferous tubule development GO:0072520 IMP     negative regulation of canonical Wnt signaling pathway GO:0090090 IEA     negative regulation of canonical Wnt signaling pathway GO:0090090 ISO     extrinsic apoptotic signaling pathway GO:0097191 IEA     extrinsic apoptotic signaling pathway GO:0097191 ISO     positive regulation of extracellular matrix assembly GO:1901203 IEA     positive regulation of extracellular matrix assembly GO:1901203 ISO     positive regulation of miRNA transcription GO:1902895 IMP     negative regulation of cardiac myofibril assembly GO:1905305 IMP Subcellular Localization     chromatin GO:0000785 IEA     chromatin GO:0000785 ISO     nucleus GO:0005634 IDA     nucleus GO:0005634 ISO     nucleoplasm GO:0005654 ISO     nucleoplasm GO:0005654 TAS     transcription regulator complex GO:0005667 IPI     transcription regulator complex GO:0005667 ISO     cytoplasm GO:0005737 IDA     cytoplasm GO:0005737 ISO     centrosome GO:0005813 IEA     centrosome GO:0005813 ISO     cytosol GO:0005829 IEA     cytosol GO:0005829 ISO     activin responsive factor complex GO:0032444 IEA     activin responsive factor complex GO:0032444 ISO     protein-containing complex GO:0032991 ISO     SMAD protein complex GO:0071141 ISO     heteromeric SMAD protein complex GO:0071144 IBA     heteromeric SMAD protein complex GO:0071144 ISO     heteromeric SMAD protein complex GO:0071144 NAS     RNA polymerase II transcription regulator complex GO:0090575 IDA