Gene description for Col5a1
Gene name collagen, type V, alpha 1
Gene symbol Col5a1
Other names/aliases AI413331
Species Mus musculus
 Database cross references - Col5a1
ExoCarta ExoCarta_12831
Vesiclepedia VP_12831
Entrez Gene 12831
UniProt O88207  
 Col5a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Col5a1
Molecular Function
    extracellular matrix structural constituent GO:0005201 IDA
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IBA
    heparin binding GO:0008201 IEA
    heparin binding GO:0008201 ISO
    extracellular matrix structural constituent conferring tensile strength GO:0030020 IBA
    extracellular matrix structural constituent conferring tensile strength GO:0030020 RCA
    proteoglycan binding GO:0043394 IEA
    proteoglycan binding GO:0043394 ISO
    platelet-derived growth factor binding GO:0048407 ISO
Biological Process
    blood vessel development GO:0001568 IMP
    heart morphogenesis GO:0003007 IGI
    cell adhesion GO:0007155 IBA
    cell adhesion GO:0007155 IEA
    cell adhesion GO:0007155 ISO
    cell migration GO:0016477 ISO
    collagen fibril organization GO:0030199 IBA
    collagen fibril organization GO:0030199 IGI
    collagen fibril organization GO:0030199 IMP
    collagen fibril organization GO:0030199 ISO
    collagen biosynthetic process GO:0032964 IEA
    collagen biosynthetic process GO:0032964 ISO
    wound healing, spreading of epidermal cells GO:0035313 IEA
    wound healing, spreading of epidermal cells GO:0035313 ISO
    tendon development GO:0035989 IGI
    tendon development GO:0035989 IMP
    skin development GO:0043588 IMP
    skin development GO:0043588 ISO
    integrin biosynthetic process GO:0045112 IEA
    integrin biosynthetic process GO:0045112 ISO
    eye morphogenesis GO:0048592 IEA
    eye morphogenesis GO:0048592 ISO
    regulation of cellular component organization GO:0051128 IMP
    supramolecular fiber organization GO:0097435 ISO
    negative regulation of endodermal cell differentiation GO:1903225 IEA
    negative regulation of endodermal cell differentiation GO:1903225 ISO
Subcellular Localization
    collagen trimer GO:0005581 IDA
    collagen trimer GO:0005581 IMP
    collagen type V trimer GO:0005588 IBA
    collagen type V trimer GO:0005588 IEA
    collagen type V trimer GO:0005588 ISO
    collagen type XI trimer GO:0005592 NAS
    basement membrane GO:0005604 IDA
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular matrix GO:0031012 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
    collagen-containing extracellular matrix GO:0062023 ISO
    collagen-containing extracellular matrix GO:0062023 NAS
 Experiment description of studies that identified Col5a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Col5a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Smad4 17128
Affinity Capture-Western Mus musculus
2 Adgre5 26364
Co-fractionation Mus musculus
3 Col4a2 12827
Co-fractionation Mus musculus
4 Smad3  
Affinity Capture-Western Mus musculus
5 Mmp2  
Co-fractionation Mus musculus
6 Serpinh1 12406
Co-fractionation Mus musculus
7 Smad7  
Affinity Capture-Western Mus musculus
8 Smad2  
Affinity Capture-Western Mus musculus
9 Pxdn 69675
Co-fractionation Mus musculus
10 Fkbp7  
Co-fractionation Mus musculus
11 Smad1  
Affinity Capture-Western Mus musculus
12 Col5a2  
Co-fractionation Mus musculus
13 Col1a2 12843
Co-fractionation Mus musculus
14 Lama5 16776
Co-fractionation Mus musculus
15 Fbn1  
Co-fractionation Mus musculus
16 Plxnb2 140570
Co-fractionation Mus musculus
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