Gene description for Cpe
Gene name carboxypeptidase E
Gene symbol Cpe
Other names/aliases CPH
Cph-1
Cph1
R74677
fat
Species Mus musculus
 Database cross references - Cpe
ExoCarta ExoCarta_12876
Vesiclepedia VP_12876
Entrez Gene 12876
UniProt Q00493  
 Cpe identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cpe
Molecular Function
    carboxypeptidase activity GO:0004180 IMP
    carboxypeptidase activity GO:0004180 ISO
    metallocarboxypeptidase activity GO:0004181 IBA
    metallocarboxypeptidase activity GO:0004181 IMP
    protein binding GO:0005515 IPI
    peptidase activity GO:0008233 TAS
    zinc ion binding GO:0008270 IEA
    protein domain specific binding GO:0019904 ISO
    neurexin family protein binding GO:0042043 IEA
    neurexin family protein binding GO:0042043 ISO
    cell adhesion molecule binding GO:0050839 IEA
    cell adhesion molecule binding GO:0050839 ISO
    cobalt ion binding GO:0050897 ISO
Biological Process
    cardiac left ventricle morphogenesis GO:0003214 IEA
    cardiac left ventricle morphogenesis GO:0003214 ISO
    peptide metabolic process GO:0006518 IBA
    peptide metabolic process GO:0006518 ISO
    Wnt signaling pathway GO:0016055 IEA
    Wnt signaling pathway GO:0016055 ISO
    protein processing GO:0016485 IBA
    protein processing GO:0016485 ISO
    insulin processing GO:0030070 IMP
    insulin processing GO:0030070 IMP
    insulin processing GO:0030070 ISO
    peptide hormone secretion GO:0030072 IMP
    protein localization to secretory granule GO:0033366 IMP
    enkephalin processing GO:0034230 ISO
    peptide catabolic process GO:0043171 ISO
    protein localization to membrane GO:0072657 IEA
    protein localization to membrane GO:0072657 ISO
    negative regulation of branching morphogenesis of a nerve GO:2000173 ISO
Subcellular Localization
    extracellular region GO:0005576 ISO
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISO
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    secretory granule GO:0030141 IDA
    secretory granule GO:0030141 ISO
    dendrite GO:0030425 ISO
    transport vesicle membrane GO:0030658 IEA
    secretory granule membrane GO:0030667 IDA
    secretory granule membrane GO:0030667 TAS
    dense core granule GO:0031045 ISO
    neuronal cell body GO:0043025 ISO
    perikaryon GO:0043204 ISO
    synaptic membrane GO:0097060 ISO
 Experiment description of studies that identified Cpe in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cpe
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRKAA1 5562
Two-hybrid Homo sapiens
2 App 11820
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Cpe is involved
No pathways found





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