Gene description for DIS3L2
Gene name DIS3 like 3'-5' exoribonuclease 2
Gene symbol DIS3L2
Other names/aliases FAM6A
PRLMNS
hDIS3L2
Species Homo sapiens
 Database cross references - DIS3L2
ExoCarta ExoCarta_129563
Vesiclepedia VP_129563
Entrez Gene 129563
HGNC 28648
MIM 614184
UniProt Q8IYB7  
 DIS3L2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for DIS3L2
Molecular Function
    3'-5'-RNA exonuclease activity GO:0000175 IBA
    3'-5'-RNA exonuclease activity GO:0000175 IDA
    magnesium ion binding GO:0000287 IDA
    RNA nuclease activity GO:0004540 IDA
    protein binding GO:0005515 IPI
    poly(U) RNA binding GO:0008266 IDA
Biological Process
    mitotic cell cycle GO:0000278 IMP
    nuclear-transcribed mRNA catabolic process GO:0000956 IMP
    mRNA catabolic process GO:0006402 IBA
    negative regulation of cell population proliferation GO:0008285 IMP
    miRNA catabolic process GO:0010587 IBA
    miRNA catabolic process GO:0010587 IDA
    miRNA catabolic process GO:0010587 ISS
    stem cell population maintenance GO:0019827 ISS
    cell division GO:0051301 IEA
    mitotic sister chromatid separation GO:0051306 IMP
    polyuridylation-dependent mRNA catabolic process GO:1990074 ISS
Subcellular Localization
    P-body GO:0000932 IBA
    P-body GO:0000932 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified DIS3L2 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for DIS3L2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPSA 3921
Co-fractionation Homo sapiens
2 MYCN  
Affinity Capture-MS Homo sapiens
3 COPS6 10980
Two-hybrid Homo sapiens
4 RELA 5970
Affinity Capture-MS Homo sapiens
5 PSMD5 5711
Co-fractionation Homo sapiens
6 FEZ1  
Two-hybrid Homo sapiens
7 BMI1  
Affinity Capture-MS Homo sapiens
8 FASN 2194
Negative Genetic Homo sapiens
9 RPLP0 6175
Affinity Capture-MS Homo sapiens
10 Mad2l1 56150
Affinity Capture-MS Mus musculus
11 HTT 3064
Co-fractionation Homo sapiens
12 SRSF6 6431
Affinity Capture-MS Homo sapiens
13 CACNG8  
Affinity Capture-MS Homo sapiens
14 Wipi2 74781
Affinity Capture-MS Mus musculus
15 HIST1H2AM 8336
Affinity Capture-MS Homo sapiens
16 NTRK1 4914
Affinity Capture-MS Homo sapiens
17 TP53 7157
Affinity Capture-MS Homo sapiens
18 VIM 7431
Two-hybrid Homo sapiens
19 TICAM1  
Affinity Capture-MS Homo sapiens
20 LIN28A  
Affinity Capture-MS Homo sapiens
21 RTP3  
Affinity Capture-MS Homo sapiens
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