Gene description for Dnmt1
Gene name DNA methyltransferase (cytosine-5) 1
Gene symbol Dnmt1
Other names/aliases Cxxc9
Dnmt
Dnmt1o
MCMT
MTase
Met-1
Met1
MommeD2
m.MmuI
Species Mus musculus
 Database cross references - Dnmt1
ExoCarta ExoCarta_13433
Vesiclepedia VP_13433
Entrez Gene 13433
UniProt P13864  
 Dnmt1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dnmt1
Molecular Function
    DNA binding GO:0003677 IBA
    DNA binding GO:0003677 IDA
    DNA binding GO:0003677 ISO
    chromatin binding GO:0003682 IDA
    RNA binding GO:0003723 IDA
    DNA (cytosine-5-)-methyltransferase activity GO:0003886 IBA
    DNA (cytosine-5-)-methyltransferase activity GO:0003886 IDA
    DNA (cytosine-5-)-methyltransferase activity GO:0003886 ISO
    protein binding GO:0005515 IPI
    methyltransferase activity GO:0008168 IDA
    zinc ion binding GO:0008270 IDA
    methyl-CpG binding GO:0008327 IDA
    DNA-methyltransferase activity GO:0009008 IMP
    DNA-methyltransferase activity GO:0009008 ISO
    DNA-methyltransferase activity GO:0009008 TAS
    protein domain specific binding GO:0019904 ISO
    nuclear estrogen receptor binding GO:0030331 ISO
    histone deacetylase binding GO:0042826 ISO
    DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates GO:0051718 TAS
    DNA (cytosine-5-)-methyltransferase activity, acting on CpN substrates GO:0051719 IEA
    DNA (cytosine-5-)-methyltransferase activity, acting on CpNpG substrates GO:0051720 IEA
    lncRNA binding GO:0106222 IDA
    promoter-specific chromatin binding GO:1990841 ISO
    promoter-specific chromatin binding GO:1990841 ISS
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    DNA methylation-dependent constitutive heterochromatin formation GO:0006346 IDA
    DNA-templated transcription GO:0006351 IMP
    response to xenobiotic stimulus GO:0009410 ISO
    regulation of gene expression GO:0010468 IMP
    positive regulation of gene expression GO:0010628 IEA
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of gene expression GO:0010629 IMP
    negative regulation of gene expression GO:0010629 ISO
    methylation GO:0032259 IEA
    regulation of cell population proliferation GO:0042127 IGI
    epigenetic programming of gene expression GO:0043045 IGI
    epigenetic programming of gene expression GO:0043045 IMP
    epigenetic programming of gene expression GO:0043045 IMP
    negative regulation of gene expression via chromosomal CpG island methylation GO:0044027 IBA
    negative regulation of gene expression via chromosomal CpG island methylation GO:0044027 IMP
    negative regulation of gene expression via chromosomal CpG island methylation GO:0044027 ISO
    negative regulation of DNA-templated transcription GO:0045892 IMP
    cellular response to amino acid stimulus GO:0071230 IDA
    cellular response to transforming growth factor beta stimulus GO:0071560 ISO
    chromosomal DNA methylation maintenance following DNA replication GO:0141119 IDA
    chromosomal DNA methylation maintenance following DNA replication GO:0141119 IGI
    chromosomal DNA methylation maintenance following DNA replication GO:0141119 IMP
    cellular response to bisphenol A GO:1903926 IDA
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 IEA
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 ISO
    negative regulation of vascular associated smooth muscle cell apoptotic process GO:1905460 IEA
    negative regulation of vascular associated smooth muscle cell apoptotic process GO:1905460 ISO
    negative regulation of vascular associated smooth muscle cell differentiation involved in phenotypic switching GO:1905931 IEA
    negative regulation of vascular associated smooth muscle cell differentiation involved in phenotypic switching GO:1905931 ISO
Subcellular Localization
    heterochromatin GO:0000792 IDA
    female germ cell nucleus GO:0001674 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 TAS
    replication fork GO:0005657 IDA
    pericentric heterochromatin GO:0005721 IDA
    cytoplasm GO:0005737 IEA
    protein-containing complex GO:0032991 ISO
    germ cell nucleus GO:0043073 IDA
 Experiment description of studies that identified Dnmt1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dnmt1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ubc  
Reconstituted Complex Mus musculus
2 Zfp57  
Co-fractionation Mus musculus
3 Trim28 21849
Co-fractionation Mus musculus
4 Cdh1 12550
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
5 Atxn1  
Affinity Capture-MS Mus musculus
6 Cul3  
Affinity Capture-MS Mus musculus
7 HDAC1 3065
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 Ddb1 13194
Affinity Capture-MS Mus musculus
9 Dnmt1 13433
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
10 Mecp2  
Co-fractionation Mus musculus
11 Pla2g2a  
Phenotypic Enhancement Mus musculus
12 Ezh2  
Affinity Capture-Western Mus musculus
13 Kat5  
Affinity Capture-Western Mus musculus
14 Mettl21e  
Affinity Capture-MS Mus musculus
15 Brap  
Affinity Capture-Western Mus musculus
16 Rela  
Two-hybrid Mus musculus
17 Zbtb16  
Affinity Capture-MS Mus musculus
18 Atm  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
19 Parp1 11545
Affinity Capture-Western Mus musculus
20 Kcnq1ot1  
Affinity Capture-RNA Mus musculus
21 Dmap1  
Affinity Capture-Western Mus musculus
22 Setdb1  
Co-fractionation Mus musculus
23 Pcna 18538
Affinity Capture-Western Mus musculus
24 Eed  
Affinity Capture-MS Mus musculus
25 Dnmt3a  
Co-fractionation Mus musculus
26 Fmr1  
Affinity Capture-MS Mus musculus
27 Uhrf1  
Affinity Capture-Western Mus musculus
Co-localization Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Co-localization Mus musculus
28 Tox3  
Two-hybrid Mus musculus
29 Fancd2  
Affinity Capture-MS Mus musculus
30 Apc  
Phenotypic Suppression Mus musculus
31 Tcf3  
Affinity Capture-MS Mus musculus
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