Gene description for CSF1
Gene name colony stimulating factor 1 (macrophage)
Gene symbol CSF1
Other names/aliases CSF-1
MCSF
Species Homo sapiens
 Database cross references - CSF1
ExoCarta ExoCarta_1435
Vesiclepedia VP_1435
Entrez Gene 1435
HGNC 2432
MIM 120420
UniProt P09603  
 CSF1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for CSF1
Molecular Function
    cytokine activity GO:0005125 IBA
    cytokine activity GO:0005125 IDA
    macrophage colony-stimulating factor receptor binding GO:0005157 ISS
    macrophage colony-stimulating factor receptor binding GO:0005157 TAS
    protein binding GO:0005515 IPI
    growth factor activity GO:0008083 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
Biological Process
    neutrophil homeostasis GO:0001780 IEA
    positive regulation of cell-matrix adhesion GO:0001954 ISS
    osteoclast proliferation GO:0002158 IEA
    response to ischemia GO:0002931 IDA
    developmental process involved in reproduction GO:0003006 ISS
    inflammatory response GO:0006954 IEA
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IDA
    Ras protein signal transduction GO:0007265 IEA
    positive regulation of cell population proliferation GO:0008284 IDA
    positive regulation of cell population proliferation GO:0008284 TAS
    positive regulation of gene expression GO:0010628 IDA
    regulation of macrophage derived foam cell differentiation GO:0010743 NAS
    positive regulation of macrophage derived foam cell differentiation GO:0010744 IDA
    positive regulation of macrophage chemotaxis GO:0010759 IDA
    monocyte differentiation GO:0030224 IEA
    macrophage differentiation GO:0030225 TAS
    regulation of ossification GO:0030278 IEA
    osteoclast differentiation GO:0030316 IBA
    osteoclast differentiation GO:0030316 IDA
    positive regulation of cell migration GO:0030335 ISS
    positive regulation of mononuclear cell proliferation GO:0032946 IDA
    monocyte homeostasis GO:0035702 IEA
    macrophage colony-stimulating factor signaling pathway GO:0038145 IEA
    positive regulation of multicellular organism growth GO:0040018 IEA
    monocyte activation GO:0042117 NAS
    positive regulation of odontogenesis of dentin-containing tooth GO:0042488 IEA
    innate immune response GO:0045087 IEA
    positive regulation of macrophage differentiation GO:0045651 IBA
    positive regulation of macrophage differentiation GO:0045651 IDA
    positive regulation of monocyte differentiation GO:0045657 ISS
    positive regulation of osteoclast differentiation GO:0045672 IDA
    positive regulation of Ras protein signal transduction GO:0046579 IEA
    homeostasis of number of cells within a tissue GO:0048873 IEA
    positive regulation of protein metabolic process GO:0051247 IDA
    branching involved in mammary gland duct morphogenesis GO:0060444 IEA
    mammary gland fat development GO:0060611 IEA
    mammary duct terminal end bud growth GO:0060763 IEA
    microglial cell proliferation GO:0061518 IEA
    macrophage homeostasis GO:0061519 IEA
    myeloid leukocyte migration GO:0097529 IEA
    positive regulation of macrophage colony-stimulating factor signaling pathway GO:1902228 IDA
    positive regulation of microglial cell migration GO:1904141 IDA
    positive regulation of macrophage migration GO:1905523 IMP
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 NAS
    nuclear body GO:0016604 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    CSF1-CSF1R complex GO:1990682 ISS
 Experiment description of studies that identified CSF1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for CSF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBL 867
Affinity Capture-Western Homo sapiens
2 NOTCH2NL 388677
Two-hybrid Homo sapiens
3 MTUS2 23281
Two-hybrid Homo sapiens
4 SGTA 6449
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 SLA2 84174
Reconstituted Complex Homo sapiens
6 FHL3 2275
Two-hybrid Homo sapiens
7 KRTAP1-1  
Two-hybrid Homo sapiens
8 CSF1R  
Reconstituted Complex Homo sapiens
9 CYSRT1 375791
Two-hybrid Homo sapiens
10 KRTAP10-8  
Two-hybrid Homo sapiens
11 AMIGO1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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