Gene description for DNAH2
Gene name dynein, axonemal, heavy chain 2
Gene symbol DNAH2
Other names/aliases DNAHC2
DNHD3
Species Homo sapiens
 Database cross references - DNAH2
ExoCarta ExoCarta_146754
Vesiclepedia VP_146754
Entrez Gene 146754
HGNC 2948
MIM 603333
UniProt Q9P225  
 DNAH2 identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for DNAH2
Molecular Function
    microtubule motor activity GO:0003777 NAS
    ATP binding GO:0005524 IEA
    minus-end-directed microtubule motor activity GO:0008569 IBA
    ATP hydrolysis activity GO:0016887 IEA
    dynein intermediate chain binding GO:0045505 IBA
    dynein light intermediate chain binding GO:0051959 IBA
Biological Process
    inner dynein arm assembly GO:0036159 IMP
    cilium-dependent cell motility GO:0060285 IMP
    cilium-dependent cell motility GO:0060285 NAS
    cilium movement involved in cell motility GO:0060294 IBA
Subcellular Localization
    axonemal dynein complex GO:0005858 NAS
    microtubule GO:0005874 IEA
    axoneme GO:0005930 IBA
    axoneme GO:0005930 IDA
    dynein complex GO:0030286 IBA
    motile cilium GO:0031514 IDA
    sperm flagellum GO:0036126 IDA
    sperm flagellum GO:0036126 IMP
    inner dynein arm GO:0036156 IMP
    9+2 motile cilium GO:0097729 IBA
 Experiment description of studies that identified DNAH2 in sEVs
1
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for DNAH2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COPS5 10987
Affinity Capture-MS Homo sapiens
2 EPX 8288
Cross-Linking-MS (XL-MS) Homo sapiens
3 ESR1  
Affinity Capture-MS Homo sapiens
4 DCX  
Cross-Linking-MS (XL-MS) Homo sapiens
5 NUMA1 4926
Cross-Linking-MS (XL-MS) Homo sapiens
6 VRK1 7443
Affinity Capture-MS Homo sapiens
7 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
8 NR3C1 2908
Proximity Label-MS Homo sapiens
9 HIST1H2BN 8341
Cross-Linking-MS (XL-MS) Homo sapiens
10 PSIP1 11168
Cross-Linking-MS (XL-MS) Homo sapiens
11 FAIM3  
Cross-Linking-MS (XL-MS) Homo sapiens
12 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
13 HNRNPA3 220988
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
14 NAA20  
Cross-Linking-MS (XL-MS) Homo sapiens
15 HNRNPA1 3178
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
16 MATR3 9782
Cross-Linking-MS (XL-MS) Homo sapiens
17 TOP2B 7155
Cross-Linking-MS (XL-MS) Homo sapiens
18 HNRNPA2B1 3181
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
19 BAG2 9532
Affinity Capture-MS Homo sapiens
20 HNRNPA1L2 144983
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml
 Pathways in which DNAH2 is involved
No pathways found





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