Gene description for Gnao1
Gene name guanine nucleotide binding protein, alpha O
Gene symbol Gnao1
Other names/aliases AW050213
Galphao
Gnao
alphaO
Species Mus musculus
 Database cross references - Gnao1
ExoCarta ExoCarta_14681
Vesiclepedia VP_14681
Entrez Gene 14681
UniProt P18872  
 Gnao1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gnao1
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 TAS
    G protein activity GO:0003925 IDA
    signaling receptor binding GO:0005102 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IMP
    GTP binding GO:0005525 ISO
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    G-protein beta/gamma-subunit complex binding GO:0031683 IEA
    G protein-coupled serotonin receptor binding GO:0031821 IBA
    G protein-coupled serotonin receptor binding GO:0031821 ISO
    mu-type opioid receptor binding GO:0031852 IBA
    mu-type opioid receptor binding GO:0031852 ISO
    GTPase activating protein binding GO:0032794 ISO
    metal ion binding GO:0046872 IEA
    corticotropin-releasing hormone receptor 1 binding GO:0051430 IBA
    corticotropin-releasing hormone receptor 1 binding GO:0051430 ISO
Biological Process
    vesicle docking involved in exocytosis GO:0006904 IMP
    G protein-coupled receptor signaling pathway GO:0007186 IMP
    G protein-coupled receptor signaling pathway GO:0007186 ISO
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 IBA
    adenylate cyclase-inhibiting serotonin receptor signaling pathway GO:0007198 IEA
    G protein-coupled dopamine receptor signaling pathway GO:0007212 IBA
    G protein-coupled dopamine receptor signaling pathway GO:0007212 IMP
    locomotory behavior GO:0007626 IMP
    regulation of heart contraction GO:0008016 IMP
    negative regulation of insulin secretion GO:0046676 IMP
    negative regulation of calcium ion transport GO:0051926 ISO
    postsynaptic modulation of chemical synaptic transmission GO:0099170 IDA
    postsynaptic modulation of chemical synaptic transmission GO:0099170 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    heterotrimeric G-protein complex GO:0005834 IBA
    heterotrimeric G-protein complex GO:0005834 TAS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
    dendrite GO:0030425 IDA
    presynaptic membrane GO:0042734 IDA
    myelin sheath GO:0043209 HDA
    myelin sheath GO:0043209 ISO
    cell body GO:0044297 IDA
    postsynaptic membrane GO:0045211 IDA
    parallel fiber to Purkinje cell synapse GO:0098688 IDA
    glutamatergic synapse GO:0098978 IDA
    GABA-ergic synapse GO:0098982 IDA
 Experiment description of studies that identified Gnao1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gnao1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Uqcrh  
Co-fractionation Mus musculus
2 Vamp2  
Affinity Capture-MS Mus musculus
3 Syngap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
4 Ndufs5  
Co-fractionation Mus musculus
5 SNCA 6622
Affinity Capture-MS Homo sapiens
6 Pcp2  
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
7 Cnksr2  
Affinity Capture-MS Mus musculus
8 Kctd13  
Affinity Capture-MS Mus musculus
9 Syp  
Co-fractionation Mus musculus
10 Prnp 19122
Co-fractionation Mus musculus
11 Rgs10  
Co-fractionation Mus musculus
12 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
13 Gpsm2  
Two-hybrid Mus musculus
14 Agap2  
Affinity Capture-MS Mus musculus
15 Htt  
Affinity Capture-MS Mus musculus
16 Ldha 16828
Two-hybrid Mus musculus
17 Snap25 20614
Co-fractionation Mus musculus
18 Cntn1 12805
Co-fractionation Mus musculus
19 Fancd2  
Affinity Capture-MS Mus musculus
20 Ric8  
Co-fractionation Mus musculus
21 Slc6a1  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Gnao1 is involved
PathwayEvidenceSource
Beta-catenin independent WNT signaling IEA Reactome
Ca2+ pathway IEA Reactome
Signal Transduction IEA Reactome
Signaling by WNT IEA Reactome





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