Gene description for CTSS
Gene name cathepsin S
Gene symbol CTSS
Other names/aliases -
Species Homo sapiens
 Database cross references - CTSS
ExoCarta ExoCarta_1520
Vesiclepedia VP_1520
Entrez Gene 1520
HGNC 2545
MIM 116845
UniProt P25774  
 CTSS identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
 Gene ontology annotations for CTSS
Molecular Function
    fibronectin binding GO:0001968 IPI
    cysteine-type endopeptidase activity GO:0004197 IBA
    cysteine-type endopeptidase activity GO:0004197 IDA
    cysteine-type endopeptidase activity GO:0004197 TAS
    serine-type endopeptidase activity GO:0004252 TAS
    collagen binding GO:0005518 IDA
    laminin binding GO:0043236 IDA
    proteoglycan binding GO:0043394 IPI
Biological Process
    toll-like receptor signaling pathway GO:0002224 TAS
    adaptive immune response GO:0002250 IEP
    proteolysis GO:0006508 IDA
    apoptotic process GO:0006915 IEA
    response to acidic pH GO:0010447 IDA
    protein processing GO:0016485 IDA
    antigen processing and presentation GO:0019882 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 IDA
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
    extracellular matrix disassembly GO:0022617 TAS
    collagen catabolic process GO:0030574 IDA
    basement membrane disassembly GO:0034769 IDA
    antigen processing and presentation of peptide antigen GO:0048002 IDA
    proteolysis involved in protein catabolic process GO:0051603 IBA
    proteolysis involved in protein catabolic process GO:0051603 IDA
    cellular response to thyroid hormone stimulus GO:0097067 IEP
    positive regulation of cation channel activity GO:2001259 IDA
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    lysosome GO:0005764 IBA
    lysosome GO:0005764 IDA
    late endosome GO:0005770 IDA
    endolysosome lumen GO:0036021 TAS
    lysosomal lumen GO:0043202 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    phagocytic vesicle GO:0045335 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    tertiary granule lumen GO:1904724 TAS
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified CTSS in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for CTSS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NIT2 56954
Co-fractionation Homo sapiens
2 AKR1C2  
Co-fractionation Homo sapiens
3 MDH1 4190
Co-fractionation Homo sapiens
4 MT1X 4501
Co-fractionation Homo sapiens
5 ALDH7A1 501
Co-fractionation Homo sapiens
6 GOT2 2806
Co-fractionation Homo sapiens
7 SERPINB3 6317
Reconstituted Complex Homo sapiens
8 SOD2 6648
Co-fractionation Homo sapiens
9 CST5 1473
Reconstituted Complex Homo sapiens
10 IRF2 3660
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 FKBP1A 2280
Co-fractionation Homo sapiens
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