Gene description for Hspa2
Gene name heat shock protein 2
Gene symbol Hspa2
Other names/aliases 70kDa
HSP70.2
HSP70A2
Hsp70-2
Species Mus musculus
 Database cross references - Hspa2
ExoCarta ExoCarta_15512
Vesiclepedia VP_15512
Entrez Gene 15512
UniProt P17156  
 Hspa2 identified in sEVs derived from the following tissue/cell type
Oligodendrocytes 21136642    
 Gene ontology annotations for Hspa2
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IBA
    enzyme binding GO:0019899 IEA
    enzyme binding GO:0019899 ISO
    heat shock protein binding GO:0031072 IBA
    protein folding chaperone GO:0044183 IBA
    tau protein binding GO:0048156 IEA
    tau protein binding GO:0048156 ISO
    unfolded protein binding GO:0051082 IEA
    unfolded protein binding GO:0051082 ISO
    protein-folding chaperone binding GO:0051087 IEA
    protein-folding chaperone binding GO:0051087 ISO
    glycolipid binding GO:0051861 IDA
    disordered domain specific binding GO:0097718 IEA
    disordered domain specific binding GO:0097718 ISO
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    positive regulation of protein phosphorylation GO:0001934 IMP
    male meiotic nuclear division GO:0007140 IMP
    male meiosis I GO:0007141 IMP
    spermatogenesis GO:0007283 IMP
    spermatid development GO:0007286 IMP
    response to heat GO:0009408 ISS
    response to cold GO:0009409 ISS
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 IMP
    positive regulation of ATP-dependent activity GO:0032781 IMP
    protein refolding GO:0042026 IBA
    protein refolding GO:0042026 IEA
    protein refolding GO:0042026 ISO
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
    synaptonemal complex disassembly GO:0070194 IMP
    negative regulation of inclusion body assembly GO:0090084 IEA
    negative regulation of inclusion body assembly GO:0090084 ISO
    positive regulation of ATPase-coupled calcium transmembrane transporter activity GO:1901896 IMP
Subcellular Localization
    synaptonemal complex GO:0000795 IDA
    male germ cell nucleus GO:0001673 IDA
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    cell surface GO:0009986 IDA
    CatSper complex GO:0036128 IDA
    myelin sheath GO:0043209 HDA
    meiotic spindle GO:0072687 IDA
 Experiment description of studies that identified Hspa2 in sEVs
1
Experiment ID 33
MISEV standards
EM|IEM
Biophysical techniques
Alix|TSG101
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Hspa2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Catsper1  
Affinity Capture-MS Mus musculus
3 Nphp4  
Affinity Capture-MS Mus musculus
4 Atxn1  
Proximity Label-MS Mus musculus
5 Cdk1 12534
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
6 Flywch2  
Co-fractionation Mus musculus
7 Traf6  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
8 Mapt  
Affinity Capture-MS Mus musculus
9 Nasp 50927
Reconstituted Complex Mus musculus
Co-purification Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
10 SOD1 6647
Affinity Capture-MS Homo sapiens
11 Ttc1  
Co-fractionation Mus musculus
12 Fancd2  
Affinity Capture-MS Mus musculus
13 H2afx 15270
Affinity Capture-MS Mus musculus
14 Ywhaz 22631
Affinity Capture-MS Mus musculus
15 Kcnma1  
Affinity Capture-MS Mus musculus
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